LOCUS BBa_K177029 4260 bp DNA linear 25-Nov-2024 DEFINITION cell Invasion device (testing) ACCESSION BBa_K177029 VERSION BBa_K177029.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..221 /label=RBS-3\Weak RBS 215..218 /label=RBS misc_feature 209..221 /label=BBa_B0032 RBS 209..221 /label=BBa_B0032 CDS 228..1817 /label=protein CDS 228..1817 /label=BBa_K177026 RBS 1826..1838 /label=RBS-3\Weak RBS 1832..1835 /label=RBS misc_feature 1826..1838 /label=BBa_B0032 RBS 1826..1838 /label=BBa_B0032 CDS 1858..4260 /label=internalin A RBS 1847..1857 /label=rbs medium CDS 1847..4260 /label=BBa_K177032 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg aaaaaaataa 241 tgctagtttt tattacactt atattagtta gtctaccaat tgcgcaacaa actgaagcaa 301 aggatgcatc tgcattcaat aaagaaaatt taatttcatc catagcacca ccagcatctc 361 cgcctgcaag tcctaagacg ccaatcgaaa agaaacacgc ggatgaaatc gataagtata 421 tacaaggatt ggattacaat aaaaacaatg tattagtata ccacggagat gcagtgacaa 481 atgtgccgcc aagaaaaggt tataaagatg gaaatgaata tatcgttgtg gagaaaaaga 541 agaaatccat caatcaaaat aatgcagata tccaagttgt gaatgcaatt tcgagcctaa 601 catatccagg tgctctcgtg aaagccaatt cggaattagt agaaaatcaa cccgatgttc 661 ttcctgtcaa acgtgattca ttaacactta gcattgattt gccaggaatg actaatcaag 721 acaataaaat tgttgtaaaa aatgctacta aatcgaacgt taacaacgca gtaaatacat 781 tagtggaaag atggaatgaa aaatatgctc aagcttatcc aaatgtaagt gcaaaaattg 841 attatgatga cgaaatggct tacagtgaat cacaattaat tgcaaaattt ggtacggcat 901 ttaaagctgt aaataatagc ttgaatgtaa acttcggcgc aatcagtgaa gggaaaatgc 961 aagaagaagt cattagtttt aaacaaattt actataacgt gaatgttaat gaacctacaa 1021 gaccttccag atttttcggc aaagctgtta ctaaagagca gttgcaagcg cttggagtga 1081 atgcagaaaa tcctcctgca tatatctcaa gtgtggcata tggccgtcaa gtttatttga 1141 aattatcaac taattcccat agtaccaaag taaaagctgc ttttgacgct gccgtaagtg 1201 ggaaatctgt ctcaggtgat gtagaactga caaatatcat caaaaattct tccttcaaag 1261 ccgtaattta cggtggctcc gcaaaagatg aagttcaaat catcgacggt aacctcggag 1321 acttacgaga tattttgaaa aaaggtgcta cttttaaccg ggaaacacca ggagttccca 1381 ttgcctatac aacaaacttc ttaaaagaca atgaattagc tgttattaaa aacaactcag 1441 aatatattga aacaacttca aaagcttata cagatggaaa aatcaacatc gatcactctg 1501 gaggatacgt tgctcaattc aacatctctt gggatgaaat aaattatgat cctgaaggta 1561 acgaaattgt tcaacataaa aactggagcg aaaacaataa aagtaagcta gctcatttca 1621 catcgtccat ctatttgcca ggtaacgcaa gaaatattaa tgtttacgct aaagaatgca 1681 ctggtttagc ttgggaatgg tggagaacgg taattgatga ccggaaccta ccgcttgtga 1741 aaaatagaaa tatctccatc tggggcacta cactttatcc gaaatatagt aatagtgtag 1801 ataatccaat cgaataatac tagagtcaca caggaaagta ctagagcaca caggaaaggt 1861 gagaaaaaaa cgatatgtat ggttgaaaag tatactagta gcaatattag tatttggcag 1921 tggagtatgg attaacacga gcaacgggac aaatgctcag gcagctacaa ttacacaaga 1981 tactcctatt aatcagattt ttacagacgc agctctagcg gaaaaaatga agacggtctt 2041 aggaaaaacg aatgtaacag acacggtctc gcaaacagat ctagaccaag ttacaacgct 2101 tcaggcggat agattaggga taaaatctat cgatggattg gaatacttga acaatttaac 2161 acaaataaat ttcagcaata atcaacttac ggatataacg ccacttaaag atttaactaa 2221 gttagttgat attttgatga ataataatca aatagcagat ataactccgc tagctaattt 2281 gacgaatcta actggtttga ctttgttcaa caatcagata acagatatag acccgcttaa 2341 aaatctaaca aatttaaatc ggctagaact atctagtaac acgattagtg atattagtgc 2401 gctttcaggt ttaactaatc tacagcaatt atcttttggt aatcaagtga cagatttaaa 2461 accattagct aatttaacaa cactagaacg actagatatt tcaagtaata aggtgtcaga 2521 tattagtgtt ctggctaaat taaccaattt agaaagtctt atcgctacta acaaccaaat 2581 aagtgatata actccacttg ggattttaac aaatttggac gaattatcct taaatggtaa 2641 ccagttaaaa gatataggca cattggcgag tttaacaaac cttacagatt tagatttagc 2701 aaataaccaa attagtaatc tagcaccact gtcgggtcta acaaaactaa ctgagttaaa 2761 acttggagct aaccaaataa gtaacatcag tcccctagca ggtttaaccg cactcactaa 2821 cttagagctt aatgaaaatc agctggaaga tattagccca atttctaacc tgaaaaatct 2881 cacatattta acgttgtact ttaataatat aagtgatata agcccagttt ctagtttaac 2941 aaagcttcaa agattatttt tctataataa caaggtaagt gacgtaagct cacttgcgaa 3001 cttaaccaat attaattggc tttcagctgg gcataaccaa attagcgatc ttacaccatt 3061 ggctaattta acaagaatca cccaactagg gttgaatgat caagcatgga caaatgcacc 3121 agtaaactac aaagcaaatg tatccattcc aaacacggtg aaaaatgtga ctggcgcttt 3181 gattgcacct gctactatta gcgatggcgg tagttacgca gaaccggata taacatggaa 3241 cttacctagt tatacaaatg aagtaagcta tacctttagc caacctgtca ctattggaaa 3301 aggaacgaca acatttagtg gaaccgtgac gcagccactt aaggcaattt ttaatgctaa 3361 gtttcatgtg gacggcaaag aaacaaccaa agaagtggaa gctgggaatt tattgactga 3421 accagctaag cccgtaaaag aaggtcacac atttgttggt tggtttgatg cccaaacagg 3481 cggaactaaa tggaatttca gtacggataa aatgccgaca aatgacatca atttatatgc 3541 acaatttagt attaacagct acacagcaac ctttgagaat gacggtgtaa caacatctca 3601 aacagtagat tatcaaggct tgttacaaga acctacacca ccaacaaaag aaggttatac 3661 tttcaaaggc tggtatgacg caaaaactgg tggtgacaag tgggatttcg caactagcaa 3721 aatgcctgct aaaaacatca ccttatatgc ccaatatagc gccaatagct atacagcaac 3781 gtttgatgtt gatggaaaat caacgactca agcagtagac tatcaaggac ttctaaaaga 3841 accaaaggca ccaacgaaag ccggatatac tttcaaaggc tggtatgacg aaaaaacaga 3901 tgggaaaaaa tgggattttg cgacggataa aatgccagca aatgacatta cgctgtacgc 3961 tcaatttacg aaaaatcctg tggcaccacc aacaactgga gggaacacac cgcctacaac 4021 aaataacggc gggaatacta caccaccttc cgcaaatata cctggaagcg acacatctaa 4081 cacatcaact gggaattcag ccagcacaac aagtacaatg aacgcttatg acccttataa 4141 ttcaaaagaa gcttcactcc ctacaactgg cgatagcgat aatgcgctct accttttgtt 4201 agggttatta gcagtaggaa ctgcaatggc tcttactaaa aaagcacgtg ctagtaaata //