BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
annotation7027
1
BBa_B0032
range7027
1
1
13
BBa_K177029
1
BBa_K177029
cell Invasion device (testing)
2009-07-21T11:00:00Z
2015-05-08T01:11:05Z
makes E. coli a good bioweapon by allowing bacteria to enter and live inside human cells
false
false
_284_
0
2582
9
Not in stock
false
false
Michael Lower
component2025499
1
BBa_B0032
component2025504
1
BBa_B0032
component2025502
1
BBa_K177026
component2025508
1
BBa_K177032
component2025491
1
BBa_R0010
annotation2025502
1
BBa_K177026
range2025502
1
228
1817
annotation2025508
1
BBa_K177032
range2025508
1
1847
4260
annotation2025504
1
BBa_B0032
range2025504
1
1826
1838
annotation2025499
1
BBa_B0032
range2025499
1
209
221
annotation2025491
1
BBa_R0010
range2025491
1
1
200
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961227
1
start
range1961227
1
173
173
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961224
1
-35
range1961224
1
137
142
BBa_K177026
1
BBa_K177026
listeriolysin O 399G->C - locus from Listeria monocytogenes
2009-07-13T11:00:00Z
2015-05-08T01:11:05Z
Listeria monocytogenes
Hemolytic protein - enables escape from endosome of a mammalian cell, following endocytosis.
false
false
_284_
0
4696
9
It's complicated
true
sequence after mutagenesis: 399G->C
false
Kamila Ornoch
annotation2011266
1
protein
range2011266
1
1
1590
BBa_K177032
1
BBa_K177032
Internalin A
2009-08-03T11:00:00Z
2015-05-08T01:11:05Z
Internalin A protein from Listeria monocytogenes 10403S
false
false
_284_
0
2582
9
Not in stock
false
Biobrick restriction sites need to be removed from the CDs by site directed mutagenesis
false
Michael Lower
annotation2014759
1
rbs medium
range2014759
1
1
11
annotation2014758
1
internalin A
range2014758
1
12
2414
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_K177026_sequence
1
atgaaaaaaataatgctagtttttattacacttatattagttagtctaccaattgcgcaacaaactgaagcaaaggatgcatctgcattcaataaagaaaatttaatttcatccatagcaccaccagcatctccgcctgcaagtcctaagacgccaatcgaaaagaaacacgcggatgaaatcgataagtatatacaaggattggattacaataaaaacaatgtattagtataccacggagatgcagtgacaaatgtgccgccaagaaaaggttataaagatggaaatgaatatatcgttgtggagaaaaagaagaaatccatcaatcaaaataatgcagatatccaagttgtgaatgcaatttcgagcctaacatatccaggtgctctcgtgaaagccaattcggaattagtagaaaatcaacccgatgttcttcctgtcaaacgtgattcattaacacttagcattgatttgccaggaatgactaatcaagacaataaaattgttgtaaaaaatgctactaaatcgaacgttaacaacgcagtaaatacattagtggaaagatggaatgaaaaatatgctcaagcttatccaaatgtaagtgcaaaaattgattatgatgacgaaatggcttacagtgaatcacaattaattgcaaaatttggtacggcatttaaagctgtaaataatagcttgaatgtaaacttcggcgcaatcagtgaagggaaaatgcaagaagaagtcattagttttaaacaaatttactataacgtgaatgttaatgaacctacaagaccttccagatttttcggcaaagctgttactaaagagcagttgcaagcgcttggagtgaatgcagaaaatcctcctgcatatatctcaagtgtggcatatggccgtcaagtttatttgaaattatcaactaattcccatagtaccaaagtaaaagctgcttttgacgctgccgtaagtgggaaatctgtctcaggtgatgtagaactgacaaatatcatcaaaaattcttccttcaaagccgtaatttacggtggctccgcaaaagatgaagttcaaatcatcgacggtaacctcggagacttacgagatattttgaaaaaaggtgctacttttaaccgggaaacaccaggagttcccattgcctatacaacaaacttcttaaaagacaatgaattagctgttattaaaaacaactcagaatatattgaaacaacttcaaaagcttatacagatggaaaaatcaacatcgatcactctggaggatacgttgctcaattcaacatctcttgggatgaaataaattatgatcctgaaggtaacgaaattgttcaacataaaaactggagcgaaaacaataaaagtaagctagctcatttcacatcgtccatctatttgccaggtaacgcaagaaatattaatgtttacgctaaagaatgcactggtttagcttgggaatggtggagaacggtaattgatgaccggaacctaccgcttgtgaaaaatagaaatatctccatctggggcactacactttatccgaaatatagtaatagtgtagataatccaatcgaataa
BBa_B0032_sequence
1
tcacacaggaaag
BBa_K177032_sequence
1
cacacaggaaaggtgagaaaaaaacgatatgtatggttgaaaagtatactagtagcaatattagtatttggcagtggagtatggattaacacgagcaacgggacaaatgctcaggcagctacaattacacaagatactcctattaatcagatttttacagacgcagctctagcggaaaaaatgaagacggtcttaggaaaaacgaatgtaacagacacggtctcgcaaacagatctagaccaagttacaacgcttcaggcggatagattagggataaaatctatcgatggattggaatacttgaacaatttaacacaaataaatttcagcaataatcaacttacggatataacgccacttaaagatttaactaagttagttgatattttgatgaataataatcaaatagcagatataactccgctagctaatttgacgaatctaactggtttgactttgttcaacaatcagataacagatatagacccgcttaaaaatctaacaaatttaaatcggctagaactatctagtaacacgattagtgatattagtgcgctttcaggtttaactaatctacagcaattatcttttggtaatcaagtgacagatttaaaaccattagctaatttaacaacactagaacgactagatatttcaagtaataaggtgtcagatattagtgttctggctaaattaaccaatttagaaagtcttatcgctactaacaaccaaataagtgatataactccacttgggattttaacaaatttggacgaattatccttaaatggtaaccagttaaaagatataggcacattggcgagtttaacaaaccttacagatttagatttagcaaataaccaaattagtaatctagcaccactgtcgggtctaacaaaactaactgagttaaaacttggagctaaccaaataagtaacatcagtcccctagcaggtttaaccgcactcactaacttagagcttaatgaaaatcagctggaagatattagcccaatttctaacctgaaaaatctcacatatttaacgttgtactttaataatataagtgatataagcccagtttctagtttaacaaagcttcaaagattatttttctataataacaaggtaagtgacgtaagctcacttgcgaacttaaccaatattaattggctttcagctgggcataaccaaattagcgatcttacaccattggctaatttaacaagaatcacccaactagggttgaatgatcaagcatggacaaatgcaccagtaaactacaaagcaaatgtatccattccaaacacggtgaaaaatgtgactggcgctttgattgcacctgctactattagcgatggcggtagttacgcagaaccggatataacatggaacttacctagttatacaaatgaagtaagctatacctttagccaacctgtcactattggaaaaggaacgacaacatttagtggaaccgtgacgcagccacttaaggcaatttttaatgctaagtttcatgtggacggcaaagaaacaaccaaagaagtggaagctgggaatttattgactgaaccagctaagcccgtaaaagaaggtcacacatttgttggttggtttgatgcccaaacaggcggaactaaatggaatttcagtacggataaaatgccgacaaatgacatcaatttatatgcacaatttagtattaacagctacacagcaacctttgagaatgacggtgtaacaacatctcaaacagtagattatcaaggcttgttacaagaacctacaccaccaacaaaagaaggttatactttcaaaggctggtatgacgcaaaaactggtggtgacaagtgggatttcgcaactagcaaaatgcctgctaaaaacatcaccttatatgcccaatatagcgccaatagctatacagcaacgtttgatgttgatggaaaatcaacgactcaagcagtagactatcaaggacttctaaaagaaccaaaggcaccaacgaaagccggatatactttcaaaggctggtatgacgaaaaaacagatgggaaaaaatgggattttgcgacggataaaatgccagcaaatgacattacgctgtacgctcaatttacgaaaaatcctgtggcaccaccaacaactggagggaacacaccgcctacaacaaataacggcgggaatactacaccaccttccgcaaatatacctggaagcgacacatctaacacatcaactgggaattcagccagcacaacaagtacaatgaacgcttatgacccttataattcaaaagaagcttcactccctacaactggcgatagcgataatgcgctctaccttttgttagggttattagcagtaggaactgcaatggctcttactaaaaaagcacgtgctagtaaata
BBa_K177029_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgaaaaaaataatgctagtttttattacacttatattagttagtctaccaattgcgcaacaaactgaagcaaaggatgcatctgcattcaataaagaaaatttaatttcatccatagcaccaccagcatctccgcctgcaagtcctaagacgccaatcgaaaagaaacacgcggatgaaatcgataagtatatacaaggattggattacaataaaaacaatgtattagtataccacggagatgcagtgacaaatgtgccgccaagaaaaggttataaagatggaaatgaatatatcgttgtggagaaaaagaagaaatccatcaatcaaaataatgcagatatccaagttgtgaatgcaatttcgagcctaacatatccaggtgctctcgtgaaagccaattcggaattagtagaaaatcaacccgatgttcttcctgtcaaacgtgattcattaacacttagcattgatttgccaggaatgactaatcaagacaataaaattgttgtaaaaaatgctactaaatcgaacgttaacaacgcagtaaatacattagtggaaagatggaatgaaaaatatgctcaagcttatccaaatgtaagtgcaaaaattgattatgatgacgaaatggcttacagtgaatcacaattaattgcaaaatttggtacggcatttaaagctgtaaataatagcttgaatgtaaacttcggcgcaatcagtgaagggaaaatgcaagaagaagtcattagttttaaacaaatttactataacgtgaatgttaatgaacctacaagaccttccagatttttcggcaaagctgttactaaagagcagttgcaagcgcttggagtgaatgcagaaaatcctcctgcatatatctcaagtgtggcatatggccgtcaagtttatttgaaattatcaactaattcccatagtaccaaagtaaaagctgcttttgacgctgccgtaagtgggaaatctgtctcaggtgatgtagaactgacaaatatcatcaaaaattcttccttcaaagccgtaatttacggtggctccgcaaaagatgaagttcaaatcatcgacggtaacctcggagacttacgagatattttgaaaaaaggtgctacttttaaccgggaaacaccaggagttcccattgcctatacaacaaacttcttaaaagacaatgaattagctgttattaaaaacaactcagaatatattgaaacaacttcaaaagcttatacagatggaaaaatcaacatcgatcactctggaggatacgttgctcaattcaacatctcttgggatgaaataaattatgatcctgaaggtaacgaaattgttcaacataaaaactggagcgaaaacaataaaagtaagctagctcatttcacatcgtccatctatttgccaggtaacgcaagaaatattaatgtttacgctaaagaatgcactggtttagcttgggaatggtggagaacggtaattgatgaccggaacctaccgcttgtgaaaaatagaaatatctccatctggggcactacactttatccgaaatatagtaatagtgtagataatccaatcgaataatactagagtcacacaggaaagtactagagcacacaggaaaggtgagaaaaaaacgatatgtatggttgaaaagtatactagtagcaatattagtatttggcagtggagtatggattaacacgagcaacgggacaaatgctcaggcagctacaattacacaagatactcctattaatcagatttttacagacgcagctctagcggaaaaaatgaagacggtcttaggaaaaacgaatgtaacagacacggtctcgcaaacagatctagaccaagttacaacgcttcaggcggatagattagggataaaatctatcgatggattggaatacttgaacaatttaacacaaataaatttcagcaataatcaacttacggatataacgccacttaaagatttaactaagttagttgatattttgatgaataataatcaaatagcagatataactccgctagctaatttgacgaatctaactggtttgactttgttcaacaatcagataacagatatagacccgcttaaaaatctaacaaatttaaatcggctagaactatctagtaacacgattagtgatattagtgcgctttcaggtttaactaatctacagcaattatcttttggtaatcaagtgacagatttaaaaccattagctaatttaacaacactagaacgactagatatttcaagtaataaggtgtcagatattagtgttctggctaaattaaccaatttagaaagtcttatcgctactaacaaccaaataagtgatataactccacttgggattttaacaaatttggacgaattatccttaaatggtaaccagttaaaagatataggcacattggcgagtttaacaaaccttacagatttagatttagcaaataaccaaattagtaatctagcaccactgtcgggtctaacaaaactaactgagttaaaacttggagctaaccaaataagtaacatcagtcccctagcaggtttaaccgcactcactaacttagagcttaatgaaaatcagctggaagatattagcccaatttctaacctgaaaaatctcacatatttaacgttgtactttaataatataagtgatataagcccagtttctagtttaacaaagcttcaaagattatttttctataataacaaggtaagtgacgtaagctcacttgcgaacttaaccaatattaattggctttcagctgggcataaccaaattagcgatcttacaccattggctaatttaacaagaatcacccaactagggttgaatgatcaagcatggacaaatgcaccagtaaactacaaagcaaatgtatccattccaaacacggtgaaaaatgtgactggcgctttgattgcacctgctactattagcgatggcggtagttacgcagaaccggatataacatggaacttacctagttatacaaatgaagtaagctatacctttagccaacctgtcactattggaaaaggaacgacaacatttagtggaaccgtgacgcagccacttaaggcaatttttaatgctaagtttcatgtggacggcaaagaaacaaccaaagaagtggaagctgggaatttattgactgaaccagctaagcccgtaaaagaaggtcacacatttgttggttggtttgatgcccaaacaggcggaactaaatggaatttcagtacggataaaatgccgacaaatgacatcaatttatatgcacaatttagtattaacagctacacagcaacctttgagaatgacggtgtaacaacatctcaaacagtagattatcaaggcttgttacaagaacctacaccaccaacaaaagaaggttatactttcaaaggctggtatgacgcaaaaactggtggtgacaagtgggatttcgcaactagcaaaatgcctgctaaaaacatcaccttatatgcccaatatagcgccaatagctatacagcaacgtttgatgttgatggaaaatcaacgactcaagcagtagactatcaaggacttctaaaagaaccaaaggcaccaacgaaagccggatatactttcaaaggctggtatgacgaaaaaacagatgggaaaaaatgggattttgcgacggataaaatgccagcaaatgacattacgctgtacgctcaatttacgaaaaatcctgtggcaccaccaacaactggagggaacacaccgcctacaacaaataacggcgggaatactacaccaccttccgcaaatatacctggaagcgacacatctaacacatcaactgggaattcagccagcacaacaagtacaatgaacgcttatgacccttataattcaaaagaagcttcactccctacaactggcgatagcgataatgcgctctaccttttgttagggttattagcagtaggaactgcaatggctcttactaaaaaagcacgtgctagtaaata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z