BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 annotation7027 1 BBa_B0032 range7027 1 1 13 BBa_K177029 1 BBa_K177029 cell Invasion device (testing) 2009-07-21T11:00:00Z 2015-05-08T01:11:05Z makes E. coli a good bioweapon by allowing bacteria to enter and live inside human cells false false _284_ 0 2582 9 Not in stock false false Michael Lower component2025499 1 BBa_B0032 component2025504 1 BBa_B0032 component2025502 1 BBa_K177026 component2025508 1 BBa_K177032 component2025491 1 BBa_R0010 annotation2025502 1 BBa_K177026 range2025502 1 228 1817 annotation2025508 1 BBa_K177032 range2025508 1 1847 4260 annotation2025504 1 BBa_B0032 range2025504 1 1826 1838 annotation2025499 1 BBa_B0032 range2025499 1 209 221 annotation2025491 1 BBa_R0010 range2025491 1 1 200 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961227 1 start range1961227 1 173 173 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961224 1 -35 range1961224 1 137 142 BBa_K177026 1 BBa_K177026 listeriolysin O 399G->C - locus from Listeria monocytogenes 2009-07-13T11:00:00Z 2015-05-08T01:11:05Z Listeria monocytogenes Hemolytic protein - enables escape from endosome of a mammalian cell, following endocytosis. false false _284_ 0 4696 9 It's complicated true sequence after mutagenesis: 399G->C false Kamila Ornoch annotation2011266 1 protein range2011266 1 1 1590 BBa_K177032 1 BBa_K177032 Internalin A 2009-08-03T11:00:00Z 2015-05-08T01:11:05Z Internalin A protein from Listeria monocytogenes 10403S false false _284_ 0 2582 9 Not in stock false Biobrick restriction sites need to be removed from the CDs by site directed mutagenesis false Michael Lower annotation2014759 1 rbs medium range2014759 1 1 11 annotation2014758 1 internalin A range2014758 1 12 2414 BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K177026_sequence 1 atgaaaaaaataatgctagtttttattacacttatattagttagtctaccaattgcgcaacaaactgaagcaaaggatgcatctgcattcaataaagaaaatttaatttcatccatagcaccaccagcatctccgcctgcaagtcctaagacgccaatcgaaaagaaacacgcggatgaaatcgataagtatatacaaggattggattacaataaaaacaatgtattagtataccacggagatgcagtgacaaatgtgccgccaagaaaaggttataaagatggaaatgaatatatcgttgtggagaaaaagaagaaatccatcaatcaaaataatgcagatatccaagttgtgaatgcaatttcgagcctaacatatccaggtgctctcgtgaaagccaattcggaattagtagaaaatcaacccgatgttcttcctgtcaaacgtgattcattaacacttagcattgatttgccaggaatgactaatcaagacaataaaattgttgtaaaaaatgctactaaatcgaacgttaacaacgcagtaaatacattagtggaaagatggaatgaaaaatatgctcaagcttatccaaatgtaagtgcaaaaattgattatgatgacgaaatggcttacagtgaatcacaattaattgcaaaatttggtacggcatttaaagctgtaaataatagcttgaatgtaaacttcggcgcaatcagtgaagggaaaatgcaagaagaagtcattagttttaaacaaatttactataacgtgaatgttaatgaacctacaagaccttccagatttttcggcaaagctgttactaaagagcagttgcaagcgcttggagtgaatgcagaaaatcctcctgcatatatctcaagtgtggcatatggccgtcaagtttatttgaaattatcaactaattcccatagtaccaaagtaaaagctgcttttgacgctgccgtaagtgggaaatctgtctcaggtgatgtagaactgacaaatatcatcaaaaattcttccttcaaagccgtaatttacggtggctccgcaaaagatgaagttcaaatcatcgacggtaacctcggagacttacgagatattttgaaaaaaggtgctacttttaaccgggaaacaccaggagttcccattgcctatacaacaaacttcttaaaagacaatgaattagctgttattaaaaacaactcagaatatattgaaacaacttcaaaagcttatacagatggaaaaatcaacatcgatcactctggaggatacgttgctcaattcaacatctcttgggatgaaataaattatgatcctgaaggtaacgaaattgttcaacataaaaactggagcgaaaacaataaaagtaagctagctcatttcacatcgtccatctatttgccaggtaacgcaagaaatattaatgtttacgctaaagaatgcactggtttagcttgggaatggtggagaacggtaattgatgaccggaacctaccgcttgtgaaaaatagaaatatctccatctggggcactacactttatccgaaatatagtaatagtgtagataatccaatcgaataa BBa_B0032_sequence 1 tcacacaggaaag BBa_K177032_sequence 1 cacacaggaaaggtgagaaaaaaacgatatgtatggttgaaaagtatactagtagcaatattagtatttggcagtggagtatggattaacacgagcaacgggacaaatgctcaggcagctacaattacacaagatactcctattaatcagatttttacagacgcagctctagcggaaaaaatgaagacggtcttaggaaaaacgaatgtaacagacacggtctcgcaaacagatctagaccaagttacaacgcttcaggcggatagattagggataaaatctatcgatggattggaatacttgaacaatttaacacaaataaatttcagcaataatcaacttacggatataacgccacttaaagatttaactaagttagttgatattttgatgaataataatcaaatagcagatataactccgctagctaatttgacgaatctaactggtttgactttgttcaacaatcagataacagatatagacccgcttaaaaatctaacaaatttaaatcggctagaactatctagtaacacgattagtgatattagtgcgctttcaggtttaactaatctacagcaattatcttttggtaatcaagtgacagatttaaaaccattagctaatttaacaacactagaacgactagatatttcaagtaataaggtgtcagatattagtgttctggctaaattaaccaatttagaaagtcttatcgctactaacaaccaaataagtgatataactccacttgggattttaacaaatttggacgaattatccttaaatggtaaccagttaaaagatataggcacattggcgagtttaacaaaccttacagatttagatttagcaaataaccaaattagtaatctagcaccactgtcgggtctaacaaaactaactgagttaaaacttggagctaaccaaataagtaacatcagtcccctagcaggtttaaccgcactcactaacttagagcttaatgaaaatcagctggaagatattagcccaatttctaacctgaaaaatctcacatatttaacgttgtactttaataatataagtgatataagcccagtttctagtttaacaaagcttcaaagattatttttctataataacaaggtaagtgacgtaagctcacttgcgaacttaaccaatattaattggctttcagctgggcataaccaaattagcgatcttacaccattggctaatttaacaagaatcacccaactagggttgaatgatcaagcatggacaaatgcaccagtaaactacaaagcaaatgtatccattccaaacacggtgaaaaatgtgactggcgctttgattgcacctgctactattagcgatggcggtagttacgcagaaccggatataacatggaacttacctagttatacaaatgaagtaagctatacctttagccaacctgtcactattggaaaaggaacgacaacatttagtggaaccgtgacgcagccacttaaggcaatttttaatgctaagtttcatgtggacggcaaagaaacaaccaaagaagtggaagctgggaatttattgactgaaccagctaagcccgtaaaagaaggtcacacatttgttggttggtttgatgcccaaacaggcggaactaaatggaatttcagtacggataaaatgccgacaaatgacatcaatttatatgcacaatttagtattaacagctacacagcaacctttgagaatgacggtgtaacaacatctcaaacagtagattatcaaggcttgttacaagaacctacaccaccaacaaaagaaggttatactttcaaaggctggtatgacgcaaaaactggtggtgacaagtgggatttcgcaactagcaaaatgcctgctaaaaacatcaccttatatgcccaatatagcgccaatagctatacagcaacgtttgatgttgatggaaaatcaacgactcaagcagtagactatcaaggacttctaaaagaaccaaaggcaccaacgaaagccggatatactttcaaaggctggtatgacgaaaaaacagatgggaaaaaatgggattttgcgacggataaaatgccagcaaatgacattacgctgtacgctcaatttacgaaaaatcctgtggcaccaccaacaactggagggaacacaccgcctacaacaaataacggcgggaatactacaccaccttccgcaaatatacctggaagcgacacatctaacacatcaactgggaattcagccagcacaacaagtacaatgaacgcttatgacccttataattcaaaagaagcttcactccctacaactggcgatagcgataatgcgctctaccttttgttagggttattagcagtaggaactgcaatggctcttactaaaaaagcacgtgctagtaaata BBa_K177029_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgaaaaaaataatgctagtttttattacacttatattagttagtctaccaattgcgcaacaaactgaagcaaaggatgcatctgcattcaataaagaaaatttaatttcatccatagcaccaccagcatctccgcctgcaagtcctaagacgccaatcgaaaagaaacacgcggatgaaatcgataagtatatacaaggattggattacaataaaaacaatgtattagtataccacggagatgcagtgacaaatgtgccgccaagaaaaggttataaagatggaaatgaatatatcgttgtggagaaaaagaagaaatccatcaatcaaaataatgcagatatccaagttgtgaatgcaatttcgagcctaacatatccaggtgctctcgtgaaagccaattcggaattagtagaaaatcaacccgatgttcttcctgtcaaacgtgattcattaacacttagcattgatttgccaggaatgactaatcaagacaataaaattgttgtaaaaaatgctactaaatcgaacgttaacaacgcagtaaatacattagtggaaagatggaatgaaaaatatgctcaagcttatccaaatgtaagtgcaaaaattgattatgatgacgaaatggcttacagtgaatcacaattaattgcaaaatttggtacggcatttaaagctgtaaataatagcttgaatgtaaacttcggcgcaatcagtgaagggaaaatgcaagaagaagtcattagttttaaacaaatttactataacgtgaatgttaatgaacctacaagaccttccagatttttcggcaaagctgttactaaagagcagttgcaagcgcttggagtgaatgcagaaaatcctcctgcatatatctcaagtgtggcatatggccgtcaagtttatttgaaattatcaactaattcccatagtaccaaagtaaaagctgcttttgacgctgccgtaagtgggaaatctgtctcaggtgatgtagaactgacaaatatcatcaaaaattcttccttcaaagccgtaatttacggtggctccgcaaaagatgaagttcaaatcatcgacggtaacctcggagacttacgagatattttgaaaaaaggtgctacttttaaccgggaaacaccaggagttcccattgcctatacaacaaacttcttaaaagacaatgaattagctgttattaaaaacaactcagaatatattgaaacaacttcaaaagcttatacagatggaaaaatcaacatcgatcactctggaggatacgttgctcaattcaacatctcttgggatgaaataaattatgatcctgaaggtaacgaaattgttcaacataaaaactggagcgaaaacaataaaagtaagctagctcatttcacatcgtccatctatttgccaggtaacgcaagaaatattaatgtttacgctaaagaatgcactggtttagcttgggaatggtggagaacggtaattgatgaccggaacctaccgcttgtgaaaaatagaaatatctccatctggggcactacactttatccgaaatatagtaatagtgtagataatccaatcgaataatactagagtcacacaggaaagtactagagcacacaggaaaggtgagaaaaaaacgatatgtatggttgaaaagtatactagtagcaatattagtatttggcagtggagtatggattaacacgagcaacgggacaaatgctcaggcagctacaattacacaagatactcctattaatcagatttttacagacgcagctctagcggaaaaaatgaagacggtcttaggaaaaacgaatgtaacagacacggtctcgcaaacagatctagaccaagttacaacgcttcaggcggatagattagggataaaatctatcgatggattggaatacttgaacaatttaacacaaataaatttcagcaataatcaacttacggatataacgccacttaaagatttaactaagttagttgatattttgatgaataataatcaaatagcagatataactccgctagctaatttgacgaatctaactggtttgactttgttcaacaatcagataacagatatagacccgcttaaaaatctaacaaatttaaatcggctagaactatctagtaacacgattagtgatattagtgcgctttcaggtttaactaatctacagcaattatcttttggtaatcaagtgacagatttaaaaccattagctaatttaacaacactagaacgactagatatttcaagtaataaggtgtcagatattagtgttctggctaaattaaccaatttagaaagtcttatcgctactaacaaccaaataagtgatataactccacttgggattttaacaaatttggacgaattatccttaaatggtaaccagttaaaagatataggcacattggcgagtttaacaaaccttacagatttagatttagcaaataaccaaattagtaatctagcaccactgtcgggtctaacaaaactaactgagttaaaacttggagctaaccaaataagtaacatcagtcccctagcaggtttaaccgcactcactaacttagagcttaatgaaaatcagctggaagatattagcccaatttctaacctgaaaaatctcacatatttaacgttgtactttaataatataagtgatataagcccagtttctagtttaacaaagcttcaaagattatttttctataataacaaggtaagtgacgtaagctcacttgcgaacttaaccaatattaattggctttcagctgggcataaccaaattagcgatcttacaccattggctaatttaacaagaatcacccaactagggttgaatgatcaagcatggacaaatgcaccagtaaactacaaagcaaatgtatccattccaaacacggtgaaaaatgtgactggcgctttgattgcacctgctactattagcgatggcggtagttacgcagaaccggatataacatggaacttacctagttatacaaatgaagtaagctatacctttagccaacctgtcactattggaaaaggaacgacaacatttagtggaaccgtgacgcagccacttaaggcaatttttaatgctaagtttcatgtggacggcaaagaaacaaccaaagaagtggaagctgggaatttattgactgaaccagctaagcccgtaaaagaaggtcacacatttgttggttggtttgatgcccaaacaggcggaactaaatggaatttcagtacggataaaatgccgacaaatgacatcaatttatatgcacaatttagtattaacagctacacagcaacctttgagaatgacggtgtaacaacatctcaaacagtagattatcaaggcttgttacaagaacctacaccaccaacaaaagaaggttatactttcaaaggctggtatgacgcaaaaactggtggtgacaagtgggatttcgcaactagcaaaatgcctgctaaaaacatcaccttatatgcccaatatagcgccaatagctatacagcaacgtttgatgttgatggaaaatcaacgactcaagcagtagactatcaaggacttctaaaagaaccaaaggcaccaacgaaagccggatatactttcaaaggctggtatgacgaaaaaacagatgggaaaaaatgggattttgcgacggataaaatgccagcaaatgacattacgctgtacgctcaatttacgaaaaatcctgtggcaccaccaacaactggagggaacacaccgcctacaacaaataacggcgggaatactacaccaccttccgcaaatatacctggaagcgacacatctaacacatcaactgggaattcagccagcacaacaagtacaatgaacgcttatgacccttataattcaaaagaagcttcactccctacaactggcgatagcgataatgcgctctaccttttgttagggttattagcagtaggaactgcaatggctcttactaaaaaagcacgtgctagtaaata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z