LOCUS BBa_K177031 3707 bp DNA linear 22-Dec-2024 DEFINITION mgtc testing device with phoQP from salmonella ACCESSION BBa_K177031 VERSION BBa_K177031.1 FEATURES Location/Qualifiers misc_feature 339..357 /label=Non-canonical PhoP binding site promoter 1..510 /label=BBa_K177021 RBS 519..531 /label=RBS-3\Weak RBS 525..528 /label=RBS misc_feature 519..531 /label=BBa_B0032 RBS 519..531 /label=BBa_B0032 CDS 538..1257 /label=GFP protein CDS 538..1257 /label=BBa_E0040 stem_loop 1277..1320 /label=stem_loop misc_feature 1266..1345 /label=BBa_B0010 terminator 1266..1345 /label=BBa_B0010 stem_loop 1361..1380 /label=T7 TE misc_feature 1387..1387 /label=stop polyA_site 1381..1394 /label=polya misc_feature 1354..1394 /label=BBa_B0012 terminator 1354..1394 /label=BBa_B0012 misc_feature 519..1394 /label=BBa_E0240 misc_feature 1..1394 /label=BBa_K177030 promoter 1403..1437 /label=BBa_J23106 RBS 1446..1458 /label=RBS-3\Weak RBS 1452..1455 /label=RBS misc_feature 1446..1458 /label=BBa_B0032 RBS 1446..1458 /label=BBa_B0032 CDS 1467..2141 /label=PhoP misc_feature 2141..3604 /label=PhoQ CDS 1467..3604 /label=BBa_K177007 stem_loop 3620..3639 /label=T7 TE misc_feature 3646..3646 /label=stop polyA_site 3640..3653 /label=polya misc_feature 3613..3653 /label=BBa_B0012 terminator 3613..3653 /label=BBa_B0012 stem_loop 3674..3696 /label=stem_loop misc_feature 3662..3707 /label=BBa_B0011 terminator 3662..3707 /label=BBa_B0011 terminator 3613..3707 /label=BBa_B0014 ORIGIN 1 agtccatttt ttctggaaga ataagtacgt gctatattta gcacacgaca aatttctgcc 61 ctgaggcaat tagcgtcgtg ttaacggcgg aggaaataaa gcgtgtttaa acatgaacat 121 gacgattacc tcgcgacaat gcgcgtgttg taacattatt gggatgacaa tcgtcacaag 181 gggagattgc tgcccaccgg ttcggcaagc agcgcaggaa tagcgtgcgt taacttgcca 241 gatgatttac gtaaaagtga ctgtccaaca tattactcct gcatgaattt gcgcttatta 301 tagtcagcca atatgttccg taaagtgcaa aggcgggttt agtgacgttc tgtttaagtt 361 tgtttgatat tttttcttat gacaaaaaaa tgacaatatt atgtcgataa caagctcgtc 421 tgaaatcatt aaacaataaa ttcctgctta ataccggcga tagctctgtt atgatgcctg 481 cacgtaaaaa aataaaacaa catgacgagg tactagagtc acacaggaaa gtactagatg 541 cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat 601 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa 661 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc 721 actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat 781 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa 841 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat 901 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg 961 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc 1021 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat 1081 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg 1141 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt 1201 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac 1261 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 1321 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 1381 tttctgcgtt tatatactag agtttacggc tagctcagtc ctaggtatag tgctagctac 1441 tagagtcaca caggaaagta ctagagctcc aggattcagg tcaccaggtc gatgccgcag 1501 aagatgccag ggaagctgat tactacctta atgaacacct tccggatatc gctattgtcg 1561 atttaggtct gccggatgaa gacggccttt ccttaatacg ccgctggcgc agcagtgatg 1621 tttcactgcc ggttctggtg ttaaccgcgc gcgaaggctg gcaggataaa gtcgaggttc 1681 tcagctccgg ggccgatgac tacgtgacga agccattcca catcgaagag gtaatggcgc 1741 gtatgcaggc gttaatgcgc cgtaatagcg gtctggcctc ccaggtgatc aacatcccgc 1801 cgttccaggt ggatctctca cgccgggaat tatccgtcaa tgaagaggtc atcaaactca 1861 cggcgttcga atacaccatt atggaaacgc ttatccgtaa caacggtaaa gtggtcagca 1921 aagattcgct gatgcttcag ctgtatccgg atgcggaact gcgggaaagt cataccattg 1981 atgttctcat ggggcgtctg cggaaaaaaa tacaggccca gtatccgcac gatgtcatta 2041 ccaccgtacg cggacaagga tatctttttg aattgcgcta atgaataaat ttgctcgcca 2101 ttttctgccg ctgtcgctgc gggttcgttt tttgctggcg acagccggcg tcgtgctggt 2161 gctttctttg gcatatggca tagtggcgct ggtcggctat agcgtaagtt ttgataaaac 2221 cacctttcgt ttgctgcgcg gcgaaagcaa cctgttttat accctcgcca aatgggaaaa 2281 taataaaatc agcgttgagc tgcctgaaaa tctggacatg caaagcccga ccatgacgct 2341 gatttacgat gaaacgggca aattattatg gacgcagcgc aacattccct ggctgattaa 2401 aagcattcaa ccggaatggt taaaaacgaa cggcttccat gaaattgaaa ccaacgtaga 2461 cgccaccagc acgctgttga gcgaagacca ttccgcgcag gaaaaactca aagaagtacg 2521 tgaagatgac gatgatgccg agatgaccca ctcggtagcg gtaaatattt atcctgccac 2581 ggcgcggatg ccgcagttaa ccatcgtggt ggtcgatacc attccgatag aactaaaacg 2641 ctcctatatg gtgtggagct ggttcgtata cgtgctggcc gccaatttac tgttagtcat 2701 tcctttactg tggatcgccg cctggtggag cttacgccct atcgaggcgc tggcgcggga 2761 agtccgcgag cttgaagatc atcaccgcga aatgctcaat ccggagacga cgcgtgagct 2821 gaccagcctt gtgcgcaacc ttaatcaact gctcaaaagc gagcgtgaac gttataacaa 2881 ataccgcacg accctgaccg acctgacgca cagtttaaaa acgccgctcg cggttttgca 2941 gagtacgtta cgctctttac gcaacgaaaa gatgagcgtc agcaaagctg aaccggtgat 3001 gctggaacag atcagccgga tttcccagca gatcggctat tatctgcatc gcgccagtat 3061 gcgcggtagc ggcgtgttgt taagccgcga actgcatccc gtcgcgccgt tgttagataa 3121 cctgatttct gcgctaaata aagtttatca gcgtaaaggg gtgaatatca gtatggatat 3181 ttcaccagaa atcagttttg tcggcgagca aaacgacttt gtcgaagtga tgggcaacgt 3241 actggacaac gcttgtaaat attgtctgga gtttgtcgag atttcggctc gccagaccga 3301 cgatcatttg catattttcg tcgaagatga cggcccaggc attccccaca gcaaacgttc 3361 cctggtgttt gatcgcggtc agcgcgccga taccctacga ccaggacaag gcgtggggct 3421 ggctgtcgcg cgcgagatta cggaacaata cgccgggcag atcattgcca gcgacagtct 3481 gctcggtggc gcccgtatgg aggtcgtttt tggccgacag catcccacac agaaagagga 3541 ataaacattt ctgtgcgttc ttccacgcat ctcgttaagc atccgttata atcggtgtca 3601 gatatactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga 3661 gagagaatat aaaaagccag attattaatc cggctttttt attattt //