##gff-version 3
##sequence-region BBa_K177031 1 3707
BBa_K177031	.	CDS	1467	3604	.	+	0	ID=BBa_K177007;Name=BBa_K177007
BBa_K177031	.	CDS	1467	2141	.	+	0	ID=annotation2000913;Name=PhoP;Parent=BBa_K177007
BBa_K177031	.	sequence_feature	2141	3604	.	+	0	ID=annotation2000914;Name=PhoQ;Parent=BBa_K177007
BBa_K177031	.	terminator	3613	3707	.	+	0	ID=BBa_B0014;Name=BBa_B0014
BBa_K177031	.	terminator	3662	3707	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_B0014
BBa_K177031	.	engineered_region	3662	3707	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K177031	.	stem_loop	3674	3696	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K177031	.	terminator	3613	3653	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0014
BBa_K177031	.	polyA_site	3640	3653	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K177031	.	stem_loop	3620	3639	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K177031	.	stop_codon	3646	3646	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K177031	.	engineered_region	3613	3653	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K177031	.	ribosome_entry_site	1446	1458	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K177031	.	engineered_region	1446	1458	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K177031	.	ribosome_entry_site	1452	1455	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K177031	.	ribosome_entry_site	1446	1458	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K177031	.	promoter	1403	1437	.	+	0	ID=BBa_J23106;Name=BBa_J23106
BBa_K177031	.	engineered_region	1	1394	.	+	0	ID=BBa_K177030;Name=BBa_K177030
BBa_K177031	.	promoter	1	510	.	+	0	ID=BBa_K177021;Name=BBa_K177021;Parent=BBa_K177030
BBa_K177031	.	conserved_region	339	357	.	+	0	ID=annotation2027404;Name=Non-canonical PhoP binding site;Parent=BBa_K177021
BBa_K177031	.	engineered_region	519	1394	.	+	0	ID=BBa_E0240;Name=BBa_E0240;Parent=BBa_K177030
BBa_K177031	.	terminator	1266	1345	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K177031	.	stem_loop	1277	1320	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K177031	.	engineered_region	1266	1345	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K177031	.	CDS	538	1257	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K177031	.	CDS	538	1257	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K177031	.	ribosome_entry_site	519	531	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K177031	.	engineered_region	519	531	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K177031	.	ribosome_entry_site	525	528	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K177031	.	ribosome_entry_site	519	531	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K177031	.	terminator	1354	1394	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K177031	.	polyA_site	1381	1394	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K177031	.	stem_loop	1361	1380	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K177031	.	stop_codon	1387	1387	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K177031	.	engineered_region	1354	1394	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K177031
agtccattttttctggaagaataagtacgtgctatatttagcacacgacaaatttctgccctgaggcaattagcgtcgt
gttaacggcggaggaaataaagcgtgtttaaacatgaacatgacgattacctcgcgacaatgcgcgtgttgtaacatta
ttgggatgacaatcgtcacaaggggagattgctgcccaccggttcggcaagcagcgcaggaatagcgtgcgttaacttg
ccagatgatttacgtaaaagtgactgtccaacatattactcctgcatgaatttgcgcttattatagtcagccaatatgt
tccgtaaagtgcaaaggcgggtttagtgacgttctgtttaagtttgtttgatattttttcttatgacaaaaaaatgaca
atattatgtcgataacaagctcgtctgaaatcattaaacaataaattcctgcttaataccggcgatagctctgttatga
tgcctgcacgtaaaaaaataaaacaacatgacgaggtactagagtcacacaggaaagtactagatgcgtaaaggagaag
aacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggaga
gggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggcca
acacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttca
agagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctga
agtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattctt
ggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagtta
acttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcga
tggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagac
cacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacggctagctcagtcct
aggtatagtgctagctactagagtcacacaggaaagtactagagctccaggattcaggtcaccaggtcgatgccgcaga
agatgccagggaagctgattactaccttaatgaacaccttccggatatcgctattgtcgatttaggtctgccggatgaa
gacggcctttccttaatacgccgctggcgcagcagtgatgtttcactgccggttctggtgttaaccgcgcgcgaaggct
ggcaggataaagtcgaggttctcagctccggggccgatgactacgtgacgaagccattccacatcgaagaggtaatggc
gcgtatgcaggcgttaatgcgccgtaatagcggtctggcctcccaggtgatcaacatcccgccgttccaggtggatctc
tcacgccgggaattatccgtcaatgaagaggtcatcaaactcacggcgttcgaatacaccattatggaaacgcttatcc
gtaacaacggtaaagtggtcagcaaagattcgctgatgcttcagctgtatccggatgcggaactgcgggaaagtcatac
cattgatgttctcatggggcgtctgcggaaaaaaatacaggcccagtatccgcacgatgtcattaccaccgtacgcgga
caaggatatctttttgaattgcgctaatgaataaatttgctcgccattttctgccgctgtcgctgcgggttcgtttttt
gctggcgacagccggcgtcgtgctggtgctttctttggcatatggcatagtggcgctggtcggctatagcgtaagtttt
gataaaaccacctttcgtttgctgcgcggcgaaagcaacctgttttataccctcgccaaatgggaaaataataaaatca
gcgttgagctgcctgaaaatctggacatgcaaagcccgaccatgacgctgatttacgatgaaacgggcaaattattatg
gacgcagcgcaacattccctggctgattaaaagcattcaaccggaatggttaaaaacgaacggcttccatgaaattgaa
accaacgtagacgccaccagcacgctgttgagcgaagaccattccgcgcaggaaaaactcaaagaagtacgtgaagatg
acgatgatgccgagatgacccactcggtagcggtaaatatttatcctgccacggcgcggatgccgcagttaaccatcgt
ggtggtcgataccattccgatagaactaaaacgctcctatatggtgtggagctggttcgtatacgtgctggccgccaat
ttactgttagtcattcctttactgtggatcgccgcctggtggagcttacgccctatcgaggcgctggcgcgggaagtcc
gcgagcttgaagatcatcaccgcgaaatgctcaatccggagacgacgcgtgagctgaccagccttgtgcgcaaccttaa
tcaactgctcaaaagcgagcgtgaacgttataacaaataccgcacgaccctgaccgacctgacgcacagtttaaaaacg
ccgctcgcggttttgcagagtacgttacgctctttacgcaacgaaaagatgagcgtcagcaaagctgaaccggtgatgc
tggaacagatcagccggatttcccagcagatcggctattatctgcatcgcgccagtatgcgcggtagcggcgtgttgtt
aagccgcgaactgcatcccgtcgcgccgttgttagataacctgatttctgcgctaaataaagtttatcagcgtaaaggg
gtgaatatcagtatggatatttcaccagaaatcagttttgtcggcgagcaaaacgactttgtcgaagtgatgggcaacg
tactggacaacgcttgtaaatattgtctggagtttgtcgagatttcggctcgccagaccgacgatcatttgcatatttt
cgtcgaagatgacggcccaggcattccccacagcaaacgttccctggtgtttgatcgcggtcagcgcgccgatacccta
cgaccaggacaaggcgtggggctggctgtcgcgcgcgagattacggaacaatacgccgggcagatcattgccagcgaca
gtctgctcggtggcgcccgtatggaggtcgtttttggccgacagcatcccacacagaaagaggaataaacatttctgtg
cgttcttccacgcatctcgttaagcatccgttataatcggtgtcagatatactagagtcacactggctcaccttcgggt
gggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt