LOCUS       BBa_K177033             2155 bp    DNA     linear       28-Jun-2024
DEFINITION  pcI lam RBS.3 LacI pcl lam RBS mRFP1 terminator
ACCESSION   BBa_K177033
VERSION     BBa_K177033.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     RBS             58..70
                     /label=RBS-3\Weak
     RBS             64..67
                     /label=RBS
     misc_feature    58..70
                     /label=BBa_B0032
     RBS             58..70
                     /label=BBa_B0032
     misc_feature    1166..1204
                     /label=LVA
     CDS             77..1204
                     /label=lacI-LVA
     misc_feature    77..1204
                     /label=BBa_C0012
     misc_feature    1205..1229
                     /label=Help:Barcodes
     CDS             77..1204
                     /label=BBa_C0012
     misc_feature    1..1229
                     /label=BBa_K177012
     promoter        1275..1280
                     /label=-10
     promoter        1252..1257
                     /label=-35
     misc_feature    1262..1278
                     /label=OR1
     misc_feature    1238..1254
                     /label=OR2
     misc_feature    1238..1286
                     /label=BBa_R0051
     promoter        1238..1286
                     /label=BBa_R0051
     misc_feature    1299..1302
                     /label=conserved
     RBS             1295..1306
                     /label=BBa_B0034
     CDS             1313..1987
                     /label=mrfp1
     misc_feature    1994..2018
                     /label=Help:Barcodes
     CDS             1313..1993
                     /label=BBa_E1010
     stem_loop       2038..2081
                     /label=stem_loop
     misc_feature    2027..2106
                     /label=BBa_B0010
     terminator      2027..2106
                     /label=BBa_B0010
     stem_loop       2122..2141
                     /label=T7 TE
     misc_feature    2148..2148
                     /label=stop
     polyA_site      2142..2155
                     /label=polya
     misc_feature    2115..2155
                     /label=BBa_B0012
     terminator      2115..2155
                     /label=BBa_B0012
     misc_feature    1295..2155
                     /label=BBa_I13507
     misc_feature    1238..2155
                     /label=BBa_I763007
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca
       61 cacaggaaag tactagatgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta
      121 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc
      181 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt
      241 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc
      301 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc
      361 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca
      421 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga
      481 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga
      541 ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga
      601 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt
      661 ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc
      721 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat
      781 gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg
      841 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg
      901 atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga
      961 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc
     1021 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc
     1081 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     1141 ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa aactacgctt tagtagctta
     1201 ataactctga tagtgctagt gtagatctct actagagtaa caccgtgcgt gttgactatt
     1261 ttacctctgg cggtgataat ggttgctact agagaaagag gagaaatact agatggcttc
     1321 ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa
     1381 cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac
     1441 cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc
     1501 gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct
     1561 gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg
     1621 tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa
     1681 actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg
     1741 ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat
     1801 gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc
     1861 taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc
     1921 ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac
     1981 cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata
     2041 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     2101 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//