##gff-version 3
##sequence-region BBa_K177033 1 2155
BBa_K177033	.	engineered_region	1238	2155	.	+	0	ID=BBa_I763007;Name=BBa_I763007
BBa_K177033	.	promoter	1238	1286	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_I763007
BBa_K177033	.	non_covalent_binding_site	1262	1278	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K177033	.	engineered_region	1238	1286	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K177033	.	promoter	1275	1280	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K177033	.	promoter	1252	1257	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K177033	.	non_covalent_binding_site	1238	1254	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K177033	.	engineered_region	1295	2155	.	+	0	ID=BBa_I13507;Name=BBa_I13507;Parent=BBa_I763007
BBa_K177033	.	CDS	1313	1993	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K177033	.	CDS	1313	1987	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K177033	.	engineered_tag	1994	2018	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K177033	.	terminator	2115	2155	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K177033	.	engineered_region	2115	2155	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K177033	.	stem_loop	2122	2141	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K177033	.	stop_codon	2148	2148	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K177033	.	polyA_site	2142	2155	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K177033	.	ribosome_entry_site	1295	1306	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K177033	.	conserved_region	1299	1302	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K177033	.	terminator	2027	2106	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K177033	.	engineered_region	2027	2106	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K177033	.	stem_loop	2038	2081	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K177033	.	sequence_feature	1	1229	.	+	0	ID=BBa_K177012;Name=BBa_K177012
BBa_K177033	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_K177012
BBa_K177033	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K177033	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K177033	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K177033	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K177033	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K177033	.	ribosome_entry_site	58	70	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K177012
BBa_K177033	.	ribosome_entry_site	58	70	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K177033	.	ribosome_entry_site	64	67	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K177033	.	engineered_region	58	70	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K177033	.	CDS	77	1204	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_K177012
BBa_K177033	.	engineered_tag	1205	1229	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K177033	.	CDS	77	1204	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K177033	.	tag	1166	1204	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K177033	.	engineered_region	77	1204	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
>BBa_K177033
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaagtactagatg
gtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtga
accaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccg
cgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcg
caaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcg
tcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatga
ccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatc
aacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcg
cgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatca
aattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgag
ggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggc
tgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccac
catcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaag
ggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcg
cgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaacta
cgctttagtagcttaataactctgatagtgctagtgtagatctctactagagtaacaccgtgcgtgttgactattttac
ctctggcggtgataatggttgctactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagt
tcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtcc
gtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccg
cagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaag
gtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacgg
tgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggt
tgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaag
acggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaac
cgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcac
tccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttata