##gff-version 3
##sequence-region BBa_K177034 1 1827
BBa_K177034	.	CDS	228	941	.	+	0	ID=BBa_K177050;Name=BBa_K177050
BBa_K177034	.	CDS	228	941	.	+	0	ID=annotation2053383;Name=cIts;Parent=BBa_K177050
BBa_K177034	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K177034	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K177034	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K177034	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K177034	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K177034	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K177034	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K177034	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K177034	.	ribosome_entry_site	950	964	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K177034	.	ribosome_entry_site	950	964	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K177034	.	ribosome_entry_site	957	961	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K177034	.	engineered_region	950	964	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K177034	.	terminator	1699	1778	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K177034	.	stem_loop	1710	1753	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K177034	.	engineered_region	1699	1778	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K177034	.	terminator	1787	1827	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K177034	.	polyA_site	1814	1827	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K177034	.	stem_loop	1794	1813	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K177034	.	stop_codon	1820	1820	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K177034	.	engineered_region	1787	1827	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K177034	.	CDS	971	1690	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K177034	.	CDS	971	1690	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K177034	.	ribosome_entry_site	209	221	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K177034	.	ribosome_entry_site	209	221	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K177034	.	ribosome_entry_site	215	218	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K177034	.	engineered_region	209	221	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
>BBa_K177034
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgagcacaa
aaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaact
tggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaat
gcattaaatgcttataacgccgcattgcttacaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgcca
gagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctca
tgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaa
aaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttc
ctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtga
tgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatc
ccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctgatactaga
gattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagat
ggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaat
ttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgc
gagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactata
tttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagt
taaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtata
catcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaa
ctagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacac
aatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattac
acatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttata