##gff-version 3
##sequence-region BBa_K177038 1 3933
BBa_K177038	.	engineered_region	1	1827	.	+	0	ID=BBa_K177034;Name=BBa_K177034
BBa_K177038	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K177034
BBa_K177038	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K177038	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K177038	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K177038	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K177038	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K177038	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K177038	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K177038	.	ribosome_entry_site	209	221	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K177034
BBa_K177038	.	ribosome_entry_site	209	221	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K177038	.	engineered_region	209	221	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K177038	.	ribosome_entry_site	215	218	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K177038	.	terminator	1699	1778	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K177034
BBa_K177038	.	engineered_region	1699	1778	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K177038	.	stem_loop	1710	1753	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K177038	.	terminator	1787	1827	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K177034
BBa_K177038	.	engineered_region	1787	1827	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K177038	.	stem_loop	1794	1813	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K177038	.	polyA_site	1814	1827	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K177038	.	stop_codon	1820	1820	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K177038	.	ribosome_entry_site	950	964	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K177034
BBa_K177038	.	ribosome_entry_site	957	961	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K177038	.	engineered_region	950	964	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K177038	.	ribosome_entry_site	950	964	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K177038	.	CDS	971	1690	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K177034
BBa_K177038	.	CDS	971	1690	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K177038	.	CDS	228	941	.	+	0	ID=BBa_K177050;Name=BBa_K177050;Parent=BBa_K177034
BBa_K177038	.	CDS	228	941	.	+	0	ID=annotation2053383;Name=cIts;Parent=BBa_K177050
BBa_K177038	.	engineered_region	3073	3933	.	+	0	ID=BBa_K094130;Name=BBa_K094130
BBa_K177038	.	CDS	3091	3796	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K094130
BBa_K177038	.	CDS	3091	3765	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K177038	.	engineered_tag	3772	3796	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K177038	.	terminator	3893	3933	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K094130
BBa_K177038	.	engineered_region	3893	3933	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K177038	.	stem_loop	3900	3919	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K177038	.	polyA_site	3920	3933	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K177038	.	stop_codon	3926	3926	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K177038	.	terminator	3805	3884	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K094130
BBa_K177038	.	engineered_region	3805	3884	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K177038	.	stem_loop	3816	3859	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K177038	.	ribosome_entry_site	3073	3084	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K094130
BBa_K177038	.	conserved_region	3077	3080	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K177038	.	sequence_feature	1836	3064	.	+	0	ID=BBa_K177012;Name=BBa_K177012
BBa_K177038	.	ribosome_entry_site	1893	1905	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K177012
BBa_K177038	.	ribosome_entry_site	1893	1905	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K177038	.	engineered_region	1893	1905	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K177038	.	ribosome_entry_site	1899	1902	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K177038	.	promoter	1836	1884	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_K177012
BBa_K177038	.	promoter	1873	1878	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K177038	.	non_covalent_binding_site	1860	1876	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K177038	.	engineered_region	1836	1884	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K177038	.	non_covalent_binding_site	1836	1852	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K177038	.	promoter	1850	1855	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K177038	.	CDS	1912	3039	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_K177012
BBa_K177038	.	engineered_region	1912	3039	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K177038	.	engineered_tag	3040	3064	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K177038	.	tag	3001	3039	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K177038	.	CDS	1912	3039	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
>BBa_K177038
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaagtactagatgagcacaa
aaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaact
tggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaat
gcattaaatgcttataacgccgcattgcttacaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgcca
gagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctca
tgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaa
aaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttc
ctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtga
tgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatc
ccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggctgatactaga
gattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagat
ggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaat
ttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgc
gagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactata
tttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagt
taaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtata
catcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaa
ctagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacac
aatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattac
acatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagtcac
acaggaaagtactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcaga
ccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagct
gaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctg
gccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcga
tggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcat
taactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtc
tctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcat
tgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataa
atatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaacc
atgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcg
ccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgtta
tatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctct
cagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgc
aaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggc
tgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggag
aaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacg
gtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaa
aggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccg
gctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtg
gtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaactt
cccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggt
gctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctaca
tggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagacta
caccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtaga
tcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata