##gff-version 3
##sequence-region BBa_K177042 1 781
BBa_K177042	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K177042	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K177042	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K177042	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K177042	.	engineered_region	20	781	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_K177042	.	sequence_alteration	261	261	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_K177042	.	engineered_region	20	781	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_K177042	.	tag	737	775	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_K177042	.	stop_codon	776	781	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_K177042	.	CDS	20	781	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
>BBa_K177042
tcacacaggaaagtactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggac
ggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagt
tcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgc
ccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatc
ttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagc
tgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtcta
tatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcag
ctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctacc
agtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcac
tctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa