LOCUS BBa_K177044 1506 bp DNA linear 13-Jan-2025 DEFINITION CI repressor from E. coli phage lambda + Tetracycline repressor (both under RBS.3) ACCESSION BBa_K177044 VERSION BBa_K177044.1 FEATURES Location/Qualifiers RBS 1..13 /label=RBS-3\Weak RBS 7..10 /label=RBS misc_feature 1..13 /label=BBa_B0032 RBS 1..13 /label=BBa_B0032 CDS 23..730 /label=cI lambda misc_feature 731..763 /label=LVA misc_feature 770..794 /label=Help:Barcodes CDS 20..794 /label=BBa_C0051 RBS 803..815 /label=RBS-3\Weak RBS 809..812 /label=RBS misc_feature 803..815 /label=BBa_B0032 RBS 803..815 /label=BBa_B0032 CDS 825..1441 /label=tetR misc_feature 1442..1475 /label=SsrA misc_feature 1482..1506 /label=Help:Barcodes CDS 822..1506 /label=BBa_C0040 ORIGIN 1 tcacacagga aagtactaga tgagcacaaa aaagaaacca ttaacacaag agcagcttga 61 ggacgcacgt cgccttaaag caatttatga aaaaaagaaa aatgaacttg gcttatccca 121 ggaatctgtc gcagacaaga tggggatggg gcagtcaggc gttggtgctt tatttaatgg 181 catcaatgca ttaaatgctt ataacgccgc attgcttgca aaaattctca aagttagcgt 241 tgaagaattt agcccttcaa tcgccagaga aatctacgag atgtatgaag cggttagtat 301 gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc aggcagggat 361 gttctcacct gagcttagaa cctttaccaa aggtgatgcg gagagatggg taagcacaac 421 caaaaaagcc agtgattctg cattctggct tgaggttgaa ggtaattcca tgaccgcacc 481 aacaggctcc aagccaagct ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc 541 tgttgagcca ggtgatttct gcatagccag acttgggggt gatgagttta ccttcaagaa 601 actgatcagg gatagcggtc aggtgttttt acaaccacta aacccacagt acccaatgat 661 cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga 721 gacgtttggc gctgcaaacg acgaaaacta cgctttagta gcttaataac gctgatagtg 781 ctagtgtaga tcgctactag agtcacacag gaaagtacta gatgtccaga ttagataaaa 841 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa 901 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa 961 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact 1021 tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta 1081 gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag 1141 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac 1201 tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg 1261 aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc 1321 cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct 1381 tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt 1441 ccgctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag tgctagtgta 1501 gatcac //