LOCUS       BBa_K177046              861 bp    DNA     linear       05-Nov-2024
DEFINITION  Tetracycline repressor under AraC regulated promoter
ACCESSION   BBa_K177046
VERSION     BBa_K177046.1  
FEATURES             Location/Qualifiers
     misc_feature    4..29
                     /label=c-amp2
     misc_feature    6..44
                     /label=araO1
     misc_feature    43..72
                     /label=c-amp1
     misc_feature    73..101
                     /label=ara1 and ara2
     promoter        113..118
                     /label=-35
     promoter        136..141
                     /label=-10
     promoter        1..149
                     /label=BBa_R0080
     RBS             158..170
                     /label=RBS-3\Weak
     RBS             164..167
                     /label=RBS
     misc_feature    158..170
                     /label=BBa_B0032
     RBS             158..170
                     /label=BBa_B0032
     CDS             180..796
                     /label=tetR
     misc_feature    797..830
                     /label=SsrA
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             177..861
                     /label=BBa_C0040
ORIGIN
        1 gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt
       61 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt
      121 tttatcgcaa ctctctactg tttctccatt actagagtca cacaggaaag tactagatgt
      181 ccagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa
      241 tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt
      301 attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata
      361 ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata
      421 acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag
      481 gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc
      541 aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt
      601 taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta
      661 ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg
      721 cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta
      781 aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc tttagtagct taataacact
      841 gatagtgcta gtgtagatca c
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