BBa_K1772001 1 BBa_K1772001 Manganese Peroxidase 2015-09-16T11:00:00Z 2015-09-19T10:25:06Z Heterobasidion irregulare TC 32-1 Protein coding sequence for Manganese Peroxidase enzyme, sequence optimized for yeast. Mechanism: oxidizing of Mn2+ ions into Mn3+ ions using an irreversible redox reaction with the aid of fungal chelators such as oxalic acid. This enzyme is specifically used by Heterobasidion irregulare TC 32-1, a variety of white rot fungus, to break down any wooden material in it???s path. Although the enzyme itself is not directly linked to lignin breakdown, the Mn3+ ions that it creates are then able to attack and oxidize organic molecules such as phenolic substrates or several lignin compounds. These lignin compounds are broken down into free radicals which quickly degrade due to their molecular instability. Note: Calcium ions and heme-B are involved with the enzyme???s mechanism. false false _2194_ 21316 22695 9 false This gene sequence excludes introns (noncoding segments) present in the original gene from Heterobasidion irregulare TC 32-1. Sequence optimization was done using IDT's codon optimizer. Illegal restriction enzyme sites (under RFC 10 definition) were detected using NEBcutter2 and removed with silent mutations. false Tianyu Tan BBa_K1772001_sequence 1 atggcattcggaacattgatagcttttgctgccctaataggtgcaagctctgcagcgattacaagaagagttgcctgccctgatggtgtcaatacggctacaaacgcagcttgttgtgctctgtttgccgtcagggatgatatccaggagaatctatttgatggaggcgaatgcggtgaagatgttcacgaatctttaagactaacatttcatgatgctataggtttctctctatccgctaatgcagccggtacatttggaggtggtggagccgatgggtctattatagttttctcctcaatcgaaaccgcattccatgcgaataatggaatcgatgaaatagtggaggaacagaaaccatttattgccagacataatattacaccaggcgatttcatacagttcgctggcgccattggtgtgtccaattgtcccggtgctccccaattggattttttgcttgggaggcctgcacccgttgctcctgcaccagatctgacggtgccggaacctttcgactccgtggactcaattttggcaagatttaacgatactggttttaacgccgcagaggtcgttgctttactggcttctcacacaatcgccgccgccgataaggtggacgtcactatccctggtacaccatttgattctacacctgaaatgttcgatacccagttctttatagaaacgcaattaagaggtactttgttccctggcactgcaggaaatcaaggtgaagttatgagtccgcttgcgggtgagttgaggctacaatccgattctgaattggctagagacaatagaacggcttgtctatggcaggcaaacgtaaatcagttagcacatatgaccagtaccttcaaagctgccatggcgaaacttgctgttcttggccaagacacaagccaaatggtagactgtagcgagttgatacctacccctcctccttttacaggtgcagcccatctgccagctggtctgacctcagctgatatagaacaagcttgcagcctgacaccttttcctaccctgccaaccgatccaggaccaattacgagtgtcgcacccgtgccacctagt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z