BBa_K1773013 1 BBa_K1773013 Lux Right promoter with strong RBS sequence and Cas3 protein 2015-08-22T11:00:00Z 2015-08-25T06:00:52Z Cas3 protein was cloned from Aggregatibacter actinomycetemcomitans D-7S strand bacterium. This part contains the Lux right promoter and strong RBS site attached to the I-F type CRISPR-Cas system Cas3 protein. Cas3 is a helicase-nuclease protein, which degrades single stranded DNA in a 3'-5' direction. true false _2195_ 27269 27269 9 false none false Sarunas Tumas component2436883 1 BBa_K1773003 component2436882 1 BBa_K1773008 annotation2436883 1 BBa_K1773003 range2436883 1 102 3388 annotation2436882 1 BBa_K1773008 range2436882 1 1 93 BBa_K1773008 1 BBa_K1773008 pLux/cI Right promoter with strong RBS 2015-08-05T11:00:00Z 2015-08-25T05:48:44Z no pLux/cI promoter with strong RBS true false _2195_ 27269 27269 9 Not in stock false Promoter and RBS were constructed with primers. false Sarunas Tumas BBa_K1773003 1 BBa_K1773003 I-F type CRISPR-Cas Cas3 gene 2015-06-08T11:00:00Z 2015-09-08T11:03:52Z A.actinomycetemcomitans D7-S I-F type CRISPR-Cas Cas3 protein Mutated EcoRI, XbaI, PstI sites false false _2195_ 27269 27269 9 false no false Sarunas Tumas BBa_K1773003_sequence 1 atgaatatcttacttgttagccaatgccaaaaaaatgcgttaacggaaacccgccgcattctcgatcaatttgccgaacggtgcggtgatcgcgtatggcaaacacccattacacaagctgggttagacaccctgcataaaatgttacgacaaacggcgcgtaaaaatacagcagtcgcttgttattggacacatgggaaaaatttaacggaattgctttggattgtgggggataaatcacaatttaacgaagaagggcgtgtgccgacaaaccgaaccaagcgcaatattttgcgcgccgaagatgaaggcggttggttacaagcctattcgatccaaattgtgaccgcacttgccgcgttattacacgatttaggcaaggcgaacaaagcctttcagttaaaattgaaacccaatgcgccttttcacgcggattattttcgtcacgagtggatttcagcccgattgtttgaagccatgattgctggttgtaaaaccgatcaggagtggctgtcacgtttggctaattgggctgaatataaagaaaattggttagcttcgcttaatattgatgataatgaagctgattttagtcatttgccgcctttggcaagaacgttgatttggctaattacttctcaccatcgcctgccattttccaatcgtctaaattatggtgagaacttcaaagaaaagacccaaaaggatatttatctgaatcaagccaagtgggatgaattttatcaatctcaattatatccgaccgagcaatgggtaaaaaacgcccaaagtacgcatcctaataaaaagcaattttggcaatttgagcatctggcgacagacagcctgtcttggcaaaaagcaatgcgccgttgggcaaagaaagcgctaaatcatccgacattacttgccatggatttttctgatccctttttcctgttagtgagtcgtttatgtttaatggttggcgatcatcattattcttctctggatgctgatgttaatttgggggataacgattttcgtgaaaaattaattgccaatacggataaaaaccatcgtgcaaaacaagcactggacgaacatttgctcggtgtttgtaaaaccgctgtgtatttttcccgtttattgcctaaattgaagactgaactgcctgcggttaaacaacataaaccctttgtcaaacgaacgtctattccgcgttttcaatggcagaatccggcatttgatttggcaaaatcccttcgggctgaaacggaaaagtgcggtctgttttcagtgaatttagccagtacggggtgtggcaaaactttgggtaatgcccgcattatgtacgccttagcggatccgcaaaaaggggcgcgatttactgctgcattaggcttgcgtgttttaaccttgcaaacgggagaagcgttacagaaaaagctaaatttaacggaggatttactggcgattttggtcggcggaagtgcggtcaaaaaattatatgaattgcagcaatcgaaagaaaataatggtagcgaatctattcagccatggtttggggaaaatgaagtgtatggtgcggattttatcgactccgccattgaagatgaaaaatttgcggcgattttacaggagcccaaagcccgaaaattattttatgctccgatcgtgacttgtacggtagatcatttaattagtgcctctgaaacggcgcgtggcggcaaatatattgtgccgatgttgcgtctgttaacatcagatttaatcttagatgaaccggatgattttaaccaagaagatttaccggcattatcccgtttagttcaccttgccggtttatttggttcccgcgtattgctttcgtcggcaacactgacaccggattttgttgcaggactattcaacgcttatcaagccggtcgggcgatttataatcgtcaacaaggaataaaccaagccgcgatttgttgtgcttggtttgatgagtttaagcaaacatcaacgaattgtgcgaatcctgaggatttcatgtttgcacatcaaagatttgtggagaaacgggctgaaaaattatgtaaagcatcggtacgccgaaaagcacaaattatgccgcttcctccattgaaaaaaatcgagaatcaattatttgatgtcgtaacctttgccgaatttttagtacaacaaactattgatttacataatgttcatagtgaatatgatcctgcgacacaaaaacaagtcagtttcggtttaattcggtttgctaatattaagccgatgattccccttgccaaacaacttttccaaatggaaatgaagccggcatggcaagattatcaaattcatttatgttgttatcattccaagcaattattgattttgcgctcaagattagaagaaaagcttgatcgcattttgaatcgtgcggacagtaaaaacgcctttacgcagcctgaagtgatggctgcaatggcaaactcacaaaccaaacatcagttatttatcgttttaggcagtccggtaaccgaggtgggacgggatcatgattacgattgggcgatcgtcgatccttcctcaatgcgttcaattattcagcttgcaggacgtgtttggcgacatcgaccggataaagttgctagccagccgaatatcactttattaccgaccaattggcgcggtttagccggacaaaccggtgtcatttttactaagccgggatttgaagatgcgaatcatccgttagaaacacatcgtacagaagagttaataccgacagctcaattagagcgtattgattccattccccgtattacaaaacgtttggattttcgaccgcacttttgcttagctgatttagagcatcaagcgacagcggatttacttaacagtcagaaagtgaattgcgtaaatgcctattggaataatgatttgagcaatcgatttacagcaaacttacctttgatgacacgttttagaaaatcacgcacacagcgcaatattgtctgcttgttggatgaagacggaaagccttatttcagagaacaggaaagtgcatgggaaacgccggataatccacaaaatgcactgccggaatttcgttatcggtcgttggacggaaagggaggactggttaaaccgtggttggattcctcgctacgtttggcattaatggaactggcggaacggttagcggaagaaagtttattatttgttgcgttgaaatatgcgacggtaagcttggctgaggatgacacgcagtggtgttttaatgaaacgctgggtttttggcaggaagatc BBa_K1773013_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatacctctggcggtgatacacatgcataattaaagaggagaaatactagagtatgaatatcttacttgttagccaatgccaaaaaaatgcgttaacggaaacccgccgcattctcgatcaatttgccgaacggtgcggtgatcgcgtatggcaaacacccattacacaagctgggttagacaccctgcataaaatgttacgacaaacggcgcgtaaaaatacagcagtcgcttgttattggacacatgggaaaaatttaacggaattgctttggattgtgggggataaatcacaatttaacgaagaagggcgtgtgccgacaaaccgaaccaagcgcaatattttgcgcgccgaagatgaaggcggttggttacaagcctattcgatccaaattgtgaccgcacttgccgcgttattacacgatttaggcaaggcgaacaaagcctttcagttaaaattgaaacccaatgcgccttttcacgcggattattttcgtcacgagtggatttcagcccgattgtttgaagccatgattgctggttgtaaaaccgatcaggagtggctgtcacgtttggctaattgggctgaatataaagaaaattggttagcttcgcttaatattgatgataatgaagctgattttagtcatttgccgcctttggcaagaacgttgatttggctaattacttctcaccatcgcctgccattttccaatcgtctaaattatggtgagaacttcaaagaaaagacccaaaaggatatttatctgaatcaagccaagtgggatgaattttatcaatctcaattatatccgaccgagcaatgggtaaaaaacgcccaaagtacgcatcctaataaaaagcaattttggcaatttgagcatctggcgacagacagcctgtcttggcaaaaagcaatgcgccgttgggcaaagaaagcgctaaatcatccgacattacttgccatggatttttctgatccctttttcctgttagtgagtcgtttatgtttaatggttggcgatcatcattattcttctctggatgctgatgttaatttgggggataacgattttcgtgaaaaattaattgccaatacggataaaaaccatcgtgcaaaacaagcactggacgaacatttgctcggtgtttgtaaaaccgctgtgtatttttcccgtttattgcctaaattgaagactgaactgcctgcggttaaacaacataaaccctttgtcaaacgaacgtctattccgcgttttcaatggcagaatccggcatttgatttggcaaaatcccttcgggctgaaacggaaaagtgcggtctgttttcagtgaatttagccagtacggggtgtggcaaaactttgggtaatgcccgcattatgtacgccttagcggatccgcaaaaaggggcgcgatttactgctgcattaggcttgcgtgttttaaccttgcaaacgggagaagcgttacagaaaaagctaaatttaacggaggatttactggcgattttggtcggcggaagtgcggtcaaaaaattatatgaattgcagcaatcgaaagaaaataatggtagcgaatctattcagccatggtttggggaaaatgaagtgtatggtgcggattttatcgactccgccattgaagatgaaaaatttgcggcgattttacaggagcccaaagcccgaaaattattttatgctccgatcgtgacttgtacggtagatcatttaattagtgcctctgaaacggcgcgtggcggcaaatatattgtgccgatgttgcgtctgttaacatcagatttaatcttagatgaaccggatgattttaaccaagaagatttaccggcattatcccgtttagttcaccttgccggtttatttggttcccgcgtattgctttcgtcggcaacactgacaccggattttgttgcaggactattcaacgcttatcaagccggtcgggcgatttataatcgtcaacaaggaataaaccaagccgcgatttgttgtgcttggtttgatgagtttaagcaaacatcaacgaattgtgcgaatcctgaggatttcatgtttgcacatcaaagatttgtggagaaacgggctgaaaaattatgtaaagcatcggtacgccgaaaagcacaaattatgccgcttcctccattgaaaaaaatcgagaatcaattatttgatgtcgtaacctttgccgaatttttagtacaacaaactattgatttacataatgttcatagtgaatatgatcctgcgacacaaaaacaagtcagtttcggtttaattcggtttgctaatattaagccgatgattccccttgccaaacaacttttccaaatggaaatgaagccggcatggcaagattatcaaattcatttatgttgttatcattccaagcaattattgattttgcgctcaagattagaagaaaagcttgatcgcattttgaatcgtgcggacagtaaaaacgcctttacgcagcctgaagtgatggctgcaatggcaaactcacaaaccaaacatcagttatttatcgttttaggcagtccggtaaccgaggtgggacgggatcatgattacgattgggcgatcgtcgatccttcctcaatgcgttcaattattcagcttgcaggacgtgtttggcgacatcgaccggataaagttgctagccagccgaatatcactttattaccgaccaattggcgcggtttagccggacaaaccggtgtcatttttactaagccgggatttgaagatgcgaatcatccgttagaaacacatcgtacagaagagttaataccgacagctcaattagagcgtattgattccattccccgtattacaaaacgtttggattttcgaccgcacttttgcttagctgatttagagcatcaagcgacagcggatttacttaacagtcagaaagtgaattgcgtaaatgcctattggaataatgatttgagcaatcgatttacagcaaacttacctttgatgacacgttttagaaaatcacgcacacagcgcaatattgtctgcttgttggatgaagacggaaagccttatttcagagaacaggaaagtgcatgggaaacgccggataatccacaaaatgcactgccggaatttcgttatcggtcgttggacggaaagggaggactggttaaaccgtggttggattcctcgctacgtttggcattaatggaactggcggaacggttagcggaagaaagtttattatttgttgcgttgaaatatgcgacggtaagcttggctgaggatgacacgcagtggtgttttaatgaaacgctgggtttttggcaggaagatc BBa_K1773008_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatacctctggcggtgatacacatgcataattaaagaggagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z