BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_K1773016
1
BBa_K1773016
pLux/cI right promoter with strong RBS
2015-08-24T11:00:00Z
2015-09-10T12:16:17Z
BBa_B0030
pLux/cI promoter with strong RBS.
false
false
_2195_
27269
27269
9
false
Assembled from oligo primers
false
Sarunas Tumas
component2437986
1
BBa_B0030
component2437984
1
BBa_K1773015
annotation2437984
1
BBa_K1773015
range2437984
1
1
78
annotation2437986
1
BBa_B0030
range2437986
1
79
93
BBa_K1773015
1
BBa_K1773015
pLux/ci lambda promoter with 10bp at 3' end for RBS attachment
2015-08-24T11:00:00Z
2015-08-25T05:56:07Z
no
pLux/ci lambda promoter with 10bp at 3' end for RBS attachment. It is a hipothetical part for assembling pLux/cI+RBS biobricks
false
false
_2195_
27269
27269
9
false
no
false
Sarunas Tumas
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K1773015_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatacctctggcggtgatacacatgcata
BBa_K1773016_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatacctctggcggtgatacacatgcataattaaagaggagaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z