BBa_B0033
1
BBa_B0033
RBS.4 (weaker) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1713
1
RBS-4\Weaker
range1713
1
1
11
annotation1714
1
RBS
range1714
1
7
10
annotation7028
1
BBa_B0033
range7028
1
1
11
BBa_K1773018
1
BBa_K1773018
pLux/cI right promoter with Weak RBS
2015-08-25T11:00:00Z
2015-08-26T02:14:10Z
iGEM parts registry
pLux/cI right promoter with Weak RBS.
false
false
_2195_
27269
27269
9
false
Assembled from oligonucleotide primers
false
Sarunas Tumas
component2438005
1
BBa_K1773015
component2438007
1
BBa_B0033
annotation2438007
1
BBa_B0033
range2438007
1
79
89
annotation2438005
1
BBa_K1773015
range2438005
1
1
78
BBa_K1773015
1
BBa_K1773015
pLux/ci lambda promoter with 10bp at 3' end for RBS attachment
2015-08-24T11:00:00Z
2015-08-25T05:56:07Z
no
pLux/ci lambda promoter with 10bp at 3' end for RBS attachment. It is a hipothetical part for assembling pLux/cI+RBS biobricks
false
false
_2195_
27269
27269
9
false
no
false
Sarunas Tumas
BBa_B0033_sequence
1
tcacacaggac
BBa_K1773018_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatacctctggcggtgatacacatgcatatcacacaggac
BBa_K1773015_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatacctctggcggtgatacacatgcata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z