LOCUS BBa_K1773019 3385 bp DNA linear 12-Dec-2024 DEFINITION Cas3 gene with pLux/cI right promoter and strong RBS ACCESSION BBa_K1773019 VERSION BBa_K1773019.1 FEATURES Location/Qualifiers promoter 1..78 /label=BBa_K1773015 RBS 79..93 /label=RBS-1\Strong RBS 86..90 /label=RBS misc_feature 79..93 /label=BBa_B0030 RBS 79..93 /label=BBa_B0030 promoter 1..93 /label=BBa_K1773016 CDS 100..3385 /label=BBa_K1773003 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atacctctgg 61 cggtgataca catgcataat taaagaggag aaatactaga tgaatatctt acttgttagc 121 caatgccaaa aaaatgcgtt aacggaaacc cgccgcattc tcgatcaatt tgccgaacgg 181 tgcggtgatc gcgtatggca aacacccatt acacaagctg ggttagacac cctgcataaa 241 atgttacgac aaacggcgcg taaaaataca gcagtcgctt gttattggac acatgggaaa 301 aatttaacgg aattgctttg gattgtgggg gataaatcac aatttaacga agaagggcgt 361 gtgccgacaa accgaaccaa gcgcaatatt ttgcgcgccg aagatgaagg cggttggtta 421 caagcctatt cgatccaaat tgtgaccgca cttgccgcgt tattacacga tttaggcaag 481 gcgaacaaag cctttcagtt aaaattgaaa cccaatgcgc cttttcacgc ggattatttt 541 cgtcacgagt ggatttcagc ccgattgttt gaagccatga ttgctggttg taaaaccgat 601 caggagtggc tgtcacgttt ggctaattgg gctgaatata aagaaaattg gttagcttcg 661 cttaatattg atgataatga agctgatttt agtcatttgc cgcctttggc aagaacgttg 721 atttggctaa ttacttctca ccatcgcctg ccattttcca atcgtctaaa ttatggtgag 781 aacttcaaag aaaagaccca aaaggatatt tatctgaatc aagccaagtg ggatgaattt 841 tatcaatctc aattatatcc gaccgagcaa tgggtaaaaa acgcccaaag tacgcatcct 901 aataaaaagc aattttggca atttgagcat ctggcgacag acagcctgtc ttggcaaaaa 961 gcaatgcgcc gttgggcaaa gaaagcgcta aatcatccga cattacttgc catggatttt 1021 tctgatccct ttttcctgtt agtgagtcgt ttatgtttaa tggttggcga tcatcattat 1081 tcttctctgg atgctgatgt taatttgggg gataacgatt ttcgtgaaaa attaattgcc 1141 aatacggata aaaaccatcg tgcaaaacaa gcactggacg aacatttgct cggtgtttgt 1201 aaaaccgctg tgtatttttc ccgtttattg cctaaattga agactgaact gcctgcggtt 1261 aaacaacata aaccctttgt caaacgaacg tctattccgc gttttcaatg gcagaatccg 1321 gcatttgatt tggcaaaatc ccttcgggct gaaacggaaa agtgcggtct gttttcagtg 1381 aatttagcca gtacggggtg tggcaaaact ttgggtaatg cccgcattat gtacgcctta 1441 gcggatccgc aaaaaggggc gcgatttact gctgcattag gcttgcgtgt tttaaccttg 1501 caaacgggag aagcgttaca gaaaaagcta aatttaacgg aggatttact ggcgattttg 1561 gtcggcggaa gtgcggtcaa aaaattatat gaattgcagc aatcgaaaga aaataatggt 1621 agcgaatcta ttcagccatg gtttggggaa aatgaagtgt atggtgcgga ttttatcgac 1681 tccgccattg aagatgaaaa atttgcggcg attttacagg agcccaaagc ccgaaaatta 1741 ttttatgctc cgatcgtgac ttgtacggta gatcatttaa ttagtgcctc tgaaacggcg 1801 cgtggcggca aatatattgt gccgatgttg cgtctgttaa catcagattt aatcttagat 1861 gaaccggatg attttaacca agaagattta ccggcattat cccgtttagt tcaccttgcc 1921 ggtttatttg gttcccgcgt attgctttcg tcggcaacac tgacaccgga ttttgttgca 1981 ggactattca acgcttatca agccggtcgg gcgatttata atcgtcaaca aggaataaac 2041 caagccgcga tttgttgtgc ttggtttgat gagtttaagc aaacatcaac gaattgtgcg 2101 aatcctgagg atttcatgtt tgcacatcaa agatttgtgg agaaacgggc tgaaaaatta 2161 tgtaaagcat cggtacgccg aaaagcacaa attatgccgc ttcctccatt gaaaaaaatc 2221 gagaatcaat tatttgatgt cgtaaccttt gccgaatttt tagtacaaca aactattgat 2281 ttacataatg ttcatagtga atatgatcct gcgacacaaa aacaagtcag tttcggttta 2341 attcggtttg ctaatattaa gccgatgatt ccccttgcca aacaactttt ccaaatggaa 2401 atgaagccgg catggcaaga ttatcaaatt catttatgtt gttatcattc caagcaatta 2461 ttgattttgc gctcaagatt agaagaaaag cttgatcgca ttttgaatcg tgcggacagt 2521 aaaaacgcct ttacgcagcc tgaagtgatg gctgcaatgg caaactcaca aaccaaacat 2581 cagttattta tcgttttagg cagtccggta accgaggtgg gacgggatca tgattacgat 2641 tgggcgatcg tcgatccttc ctcaatgcgt tcaattattc agcttgcagg acgtgtttgg 2701 cgacatcgac cggataaagt tgctagccag ccgaatatca ctttattacc gaccaattgg 2761 cgcggtttag ccggacaaac cggtgtcatt tttactaagc cgggatttga agatgcgaat 2821 catccgttag aaacacatcg tacagaagag ttaataccga cagctcaatt agagcgtatt 2881 gattccattc cccgtattac aaaacgtttg gattttcgac cgcacttttg cttagctgat 2941 ttagagcatc aagcgacagc ggatttactt aacagtcaga aagtgaattg cgtaaatgcc 3001 tattggaata atgatttgag caatcgattt acagcaaact tacctttgat gacacgtttt 3061 agaaaatcac gcacacagcg caatattgtc tgcttgttgg atgaagacgg aaagccttat 3121 ttcagagaac aggaaagtgc atgggaaacg ccggataatc cacaaaatgc actgccggaa 3181 tttcgttatc ggtcgttgga cggaaaggga ggactggtta aaccgtggtt ggattcctcg 3241 ctacgtttgg cattaatgga actggcggaa cggttagcgg aagaaagttt attatttgtt 3301 gcgttgaaat atgcgacggt aagcttggct gaggatgaca cgcagtggtg ttttaatgaa 3361 acgctgggtt tttggcagga agatc //