BBa_K1773020 1 BBa_K1773020 Cas3 gene with pLux/cI right promoter and Medium RBS 2015-08-25T11:00:00Z 2015-08-26T03:52:20Z I-F type CRISPR-Cas system was cloned from Aggregatibacter actinomycetemcomitans D7-S strand. Gene encoding I-F type CRISPR_Cas system Cas3 protein, which is an helicase and ssDNA nuclease. Attached there is a pLux/cI right promoter and Medium RBS site. In the I-F type CRISPR-Cas system Cascade complex distinguishes target DNA, and rectruits Cas3 protein, which acts as a nuclease and helicase, which hidrolises ssDNA in a 3'-5' direction. false false _2195_ 27269 27269 9 false no false Sarunas Tumas component2438040 1 BBa_K1773017 component2438041 1 BBa_K1773003 annotation2438040 1 BBa_K1773017 range2438040 1 1 91 annotation2438041 1 BBa_K1773003 range2438041 1 98 3383 BBa_K1773003 1 BBa_K1773003 I-F type CRISPR-Cas Cas3 gene 2015-06-08T11:00:00Z 2015-09-08T11:03:52Z A.actinomycetemcomitans D7-S I-F type CRISPR-Cas Cas3 protein Mutated EcoRI, XbaI, PstI sites false false _2195_ 27269 27269 9 false no false Sarunas Tumas BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1710 1 RBS range1710 1 7 10 annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation7027 1 BBa_B0032 range7027 1 1 13 BBa_K1773017 1 BBa_K1773017 pLux/cI right promoter with medium RBS 2015-08-25T11:00:00Z 2015-08-26T02:05:21Z From parts registry pLux/cI right promoter with medium RBS. false false _2195_ 27269 27269 9 false assembled with oligonucleotide primers false Sarunas Tumas component2437997 1 BBa_K1773015 component2437999 1 BBa_B0032 annotation2437997 1 BBa_K1773015 range2437997 1 1 78 annotation2437999 1 BBa_B0032 range2437999 1 79 91 BBa_K1773015 1 BBa_K1773015 pLux/ci lambda promoter with 10bp at 3' end for RBS attachment 2015-08-24T11:00:00Z 2015-08-25T05:56:07Z no pLux/ci lambda promoter with 10bp at 3' end for RBS attachment. It is a hipothetical part for assembling pLux/cI+RBS biobricks false false _2195_ 27269 27269 9 false no false Sarunas Tumas BBa_K1773017_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatacctctggcggtgatacacatgcatatcacacaggaaag BBa_K1773003_sequence 1 atgaatatcttacttgttagccaatgccaaaaaaatgcgttaacggaaacccgccgcattctcgatcaatttgccgaacggtgcggtgatcgcgtatggcaaacacccattacacaagctgggttagacaccctgcataaaatgttacgacaaacggcgcgtaaaaatacagcagtcgcttgttattggacacatgggaaaaatttaacggaattgctttggattgtgggggataaatcacaatttaacgaagaagggcgtgtgccgacaaaccgaaccaagcgcaatattttgcgcgccgaagatgaaggcggttggttacaagcctattcgatccaaattgtgaccgcacttgccgcgttattacacgatttaggcaaggcgaacaaagcctttcagttaaaattgaaacccaatgcgccttttcacgcggattattttcgtcacgagtggatttcagcccgattgtttgaagccatgattgctggttgtaaaaccgatcaggagtggctgtcacgtttggctaattgggctgaatataaagaaaattggttagcttcgcttaatattgatgataatgaagctgattttagtcatttgccgcctttggcaagaacgttgatttggctaattacttctcaccatcgcctgccattttccaatcgtctaaattatggtgagaacttcaaagaaaagacccaaaaggatatttatctgaatcaagccaagtgggatgaattttatcaatctcaattatatccgaccgagcaatgggtaaaaaacgcccaaagtacgcatcctaataaaaagcaattttggcaatttgagcatctggcgacagacagcctgtcttggcaaaaagcaatgcgccgttgggcaaagaaagcgctaaatcatccgacattacttgccatggatttttctgatccctttttcctgttagtgagtcgtttatgtttaatggttggcgatcatcattattcttctctggatgctgatgttaatttgggggataacgattttcgtgaaaaattaattgccaatacggataaaaaccatcgtgcaaaacaagcactggacgaacatttgctcggtgtttgtaaaaccgctgtgtatttttcccgtttattgcctaaattgaagactgaactgcctgcggttaaacaacataaaccctttgtcaaacgaacgtctattccgcgttttcaatggcagaatccggcatttgatttggcaaaatcccttcgggctgaaacggaaaagtgcggtctgttttcagtgaatttagccagtacggggtgtggcaaaactttgggtaatgcccgcattatgtacgccttagcggatccgcaaaaaggggcgcgatttactgctgcattaggcttgcgtgttttaaccttgcaaacgggagaagcgttacagaaaaagctaaatttaacggaggatttactggcgattttggtcggcggaagtgcggtcaaaaaattatatgaattgcagcaatcgaaagaaaataatggtagcgaatctattcagccatggtttggggaaaatgaagtgtatggtgcggattttatcgactccgccattgaagatgaaaaatttgcggcgattttacaggagcccaaagcccgaaaattattttatgctccgatcgtgacttgtacggtagatcatttaattagtgcctctgaaacggcgcgtggcggcaaatatattgtgccgatgttgcgtctgttaacatcagatttaatcttagatgaaccggatgattttaaccaagaagatttaccggcattatcccgtttagttcaccttgccggtttatttggttcccgcgtattgctttcgtcggcaacactgacaccggattttgttgcaggactattcaacgcttatcaagccggtcgggcgatttataatcgtcaacaaggaataaaccaagccgcgatttgttgtgcttggtttgatgagtttaagcaaacatcaacgaattgtgcgaatcctgaggatttcatgtttgcacatcaaagatttgtggagaaacgggctgaaaaattatgtaaagcatcggtacgccgaaaagcacaaattatgccgcttcctccattgaaaaaaatcgagaatcaattatttgatgtcgtaacctttgccgaatttttagtacaacaaactattgatttacataatgttcatagtgaatatgatcctgcgacacaaaaacaagtcagtttcggtttaattcggtttgctaatattaagccgatgattccccttgccaaacaacttttccaaatggaaatgaagccggcatggcaagattatcaaattcatttatgttgttatcattccaagcaattattgattttgcgctcaagattagaagaaaagcttgatcgcattttgaatcgtgcggacagtaaaaacgcctttacgcagcctgaagtgatggctgcaatggcaaactcacaaaccaaacatcagttatttatcgttttaggcagtccggtaaccgaggtgggacgggatcatgattacgattgggcgatcgtcgatccttcctcaatgcgttcaattattcagcttgcaggacgtgtttggcgacatcgaccggataaagttgctagccagccgaatatcactttattaccgaccaattggcgcggtttagccggacaaaccggtgtcatttttactaagccgggatttgaagatgcgaatcatccgttagaaacacatcgtacagaagagttaataccgacagctcaattagagcgtattgattccattccccgtattacaaaacgtttggattttcgaccgcacttttgcttagctgatttagagcatcaagcgacagcggatttacttaacagtcagaaagtgaattgcgtaaatgcctattggaataatgatttgagcaatcgatttacagcaaacttacctttgatgacacgttttagaaaatcacgcacacagcgcaatattgtctgcttgttggatgaagacggaaagccttatttcagagaacaggaaagtgcatgggaaacgccggataatccacaaaatgcactgccggaatttcgttatcggtcgttggacggaaagggaggactggttaaaccgtggttggattcctcgctacgtttggcattaatggaactggcggaacggttagcggaagaaagtttattatttgttgcgttgaaatatgcgacggtaagcttggctgaggatgacacgcagtggtgttttaatgaaacgctgggtttttggcaggaagatc BBa_K1773020_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatacctctggcggtgatacacatgcatatcacacaggaaagtactagatgaatatcttacttgttagccaatgccaaaaaaatgcgttaacggaaacccgccgcattctcgatcaatttgccgaacggtgcggtgatcgcgtatggcaaacacccattacacaagctgggttagacaccctgcataaaatgttacgacaaacggcgcgtaaaaatacagcagtcgcttgttattggacacatgggaaaaatttaacggaattgctttggattgtgggggataaatcacaatttaacgaagaagggcgtgtgccgacaaaccgaaccaagcgcaatattttgcgcgccgaagatgaaggcggttggttacaagcctattcgatccaaattgtgaccgcacttgccgcgttattacacgatttaggcaaggcgaacaaagcctttcagttaaaattgaaacccaatgcgccttttcacgcggattattttcgtcacgagtggatttcagcccgattgtttgaagccatgattgctggttgtaaaaccgatcaggagtggctgtcacgtttggctaattgggctgaatataaagaaaattggttagcttcgcttaatattgatgataatgaagctgattttagtcatttgccgcctttggcaagaacgttgatttggctaattacttctcaccatcgcctgccattttccaatcgtctaaattatggtgagaacttcaaagaaaagacccaaaaggatatttatctgaatcaagccaagtgggatgaattttatcaatctcaattatatccgaccgagcaatgggtaaaaaacgcccaaagtacgcatcctaataaaaagcaattttggcaatttgagcatctggcgacagacagcctgtcttggcaaaaagcaatgcgccgttgggcaaagaaagcgctaaatcatccgacattacttgccatggatttttctgatccctttttcctgttagtgagtcgtttatgtttaatggttggcgatcatcattattcttctctggatgctgatgttaatttgggggataacgattttcgtgaaaaattaattgccaatacggataaaaaccatcgtgcaaaacaagcactggacgaacatttgctcggtgtttgtaaaaccgctgtgtatttttcccgtttattgcctaaattgaagactgaactgcctgcggttaaacaacataaaccctttgtcaaacgaacgtctattccgcgttttcaatggcagaatccggcatttgatttggcaaaatcccttcgggctgaaacggaaaagtgcggtctgttttcagtgaatttagccagtacggggtgtggcaaaactttgggtaatgcccgcattatgtacgccttagcggatccgcaaaaaggggcgcgatttactgctgcattaggcttgcgtgttttaaccttgcaaacgggagaagcgttacagaaaaagctaaatttaacggaggatttactggcgattttggtcggcggaagtgcggtcaaaaaattatatgaattgcagcaatcgaaagaaaataatggtagcgaatctattcagccatggtttggggaaaatgaagtgtatggtgcggattttatcgactccgccattgaagatgaaaaatttgcggcgattttacaggagcccaaagcccgaaaattattttatgctccgatcgtgacttgtacggtagatcatttaattagtgcctctgaaacggcgcgtggcggcaaatatattgtgccgatgttgcgtctgttaacatcagatttaatcttagatgaaccggatgattttaaccaagaagatttaccggcattatcccgtttagttcaccttgccggtttatttggttcccgcgtattgctttcgtcggcaacactgacaccggattttgttgcaggactattcaacgcttatcaagccggtcgggcgatttataatcgtcaacaaggaataaaccaagccgcgatttgttgtgcttggtttgatgagtttaagcaaacatcaacgaattgtgcgaatcctgaggatttcatgtttgcacatcaaagatttgtggagaaacgggctgaaaaattatgtaaagcatcggtacgccgaaaagcacaaattatgccgcttcctccattgaaaaaaatcgagaatcaattatttgatgtcgtaacctttgccgaatttttagtacaacaaactattgatttacataatgttcatagtgaatatgatcctgcgacacaaaaacaagtcagtttcggtttaattcggtttgctaatattaagccgatgattccccttgccaaacaacttttccaaatggaaatgaagccggcatggcaagattatcaaattcatttatgttgttatcattccaagcaattattgattttgcgctcaagattagaagaaaagcttgatcgcattttgaatcgtgcggacagtaaaaacgcctttacgcagcctgaagtgatggctgcaatggcaaactcacaaaccaaacatcagttatttatcgttttaggcagtccggtaaccgaggtgggacgggatcatgattacgattgggcgatcgtcgatccttcctcaatgcgttcaattattcagcttgcaggacgtgtttggcgacatcgaccggataaagttgctagccagccgaatatcactttattaccgaccaattggcgcggtttagccggacaaaccggtgtcatttttactaagccgggatttgaagatgcgaatcatccgttagaaacacatcgtacagaagagttaataccgacagctcaattagagcgtattgattccattccccgtattacaaaacgtttggattttcgaccgcacttttgcttagctgatttagagcatcaagcgacagcggatttacttaacagtcagaaagtgaattgcgtaaatgcctattggaataatgatttgagcaatcgatttacagcaaacttacctttgatgacacgttttagaaaatcacgcacacagcgcaatattgtctgcttgttggatgaagacggaaagccttatttcagagaacaggaaagtgcatgggaaacgccggataatccacaaaatgcactgccggaatttcgttatcggtcgttggacggaaagggaggactggttaaaccgtggttggattcctcgctacgtttggcattaatggaactggcggaacggttagcggaagaaagtttattatttgttgcgttgaaatatgcgacggtaagcttggctgaggatgacacgcagtggtgttttaatgaaacgctgggtttttggcaggaagatc BBa_B0032_sequence 1 tcacacaggaaag BBa_K1773015_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatacctctggcggtgatacacatgcata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z