LOCUS       BBa_K1773023            3997 bp    DNA     linear       26-Nov-2024
DEFINITION  Cascade with pLux/cI right promoter and Medium RBS
ACCESSION   BBa_K1773023
VERSION     BBa_K1773023.1  
FEATURES             Location/Qualifiers
     promoter        1..78
                     /label=BBa_K1773015
     RBS             79..91
                     /label=RBS-3\Weak
     RBS             85..88
                     /label=RBS
     misc_feature    79..91
                     /label=BBa_B0032
     RBS             79..91
                     /label=BBa_B0032
     promoter        1..91
                     /label=BBa_K1773017
     CDS             100..3997
                     /label=BBa_K1773005
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atacctctgg
       61 cggtgataca catgcatatc acacaggaaa gtactagagc atgataacta aaacaacagt
      121 agtaaaagcc attcaacaat ttcttgttga tcaaaatcta aagaaatccg gaaccgatag
      181 aaagaaacta gaaaatgcct ctctttcaga atcagaaaaa gaagcattat tgaaaaaaat
      241 cgccgagtat gatgaaaaat accgttttga tgtttgggtt gaaaatgccg ctacggtaat
      301 ggcaaagcag ttgtatttcg gtacgcacat ttcccgtgga atccactctt cctcaaaagg
      361 tgataatgtt aattttcaat ctgatttgac gctttccgat acacttgtcg gttcgcaatc
      421 aatacctaag ttagaacttg atgcaaacgg gaatgcggct gcgcttccct tagccggttt
      481 ctttaatatt attgttgatg aggaaaaaaa tacgacactt aaagaattgt tacttaatga
      541 cgattcttgc cttgaaggct gtttcgctga tgatcctgaa ttatccaagc aatataagat
      601 ttgctttcaa aatgcattaa aaggggattt aacaaaaccg agaaccgacg agcgtaataa
      661 acaactccta tgggcaaata atttcgattc tggaaagaat gcttatattt gcctaattcc
      721 cttgtatccg tcctcattaa ccagtcattt ttaccataaa attaatcaat tgcgttattc
      781 tgactataac aagcaatcat catccaatcg ctataaaaaa gatgtatcgc aacttcctta
      841 tgtatcatta caaaatatag gggtaactgt gttgggcgga acaaagccac agaatgtcag
      901 ccaacttacc agtaaccaag ggggacggaa ttatttgctt ccttcacttc cgcctatttt
      961 taaatctcaa gaaaaaatac gtttaactca aaaacaaacc accattttta atcgtcgttt
     1021 ggcttatgct tgtcgtgaag gattaaatat gctgtatgaa gtcgttgaag cagaaaaaaa
     1081 tatttatccg gaaagagata aacgtaaatt ggcgttagag ttgattgctc acactgtttt
     1141 aatgcaagcg aagtacattc aaaataactc ccctacaggt tggagcaaag actatcacct
     1201 taatgaatat caaaaatatt ggcttgatcc acaccgtgca gaactggatg gcgaagataa
     1261 ttttagacat aacattgagc aaaaccattg gaccgccgaa attatccgtc aatttgcact
     1321 ttgggttaat gcttgcctta aatggcgttt taaatctatt caacaggatt ttactacacc
     1381 tgaatataac caatggtgca gagaaatgga aaaagccatt gctgcacaag cccggttaag
     1441 tcaataagga tcaaatatga acactgattt ctatttactt ttcaagcata ttaaaatcca
     1501 gtccgccaat gcgatttcag gtcctttaag ctatggtttt cctgccgtta gcggctttgt
     1561 cggtgcgata catgcgttaa gtcgtaagct aaatgaaaaa gccatgttag atggtgtaac
     1621 aattgcttgc cataactatg atttacaggc ttaccaatcc acaccatttt ccgaccgcac
     1681 ttttatccag acccgtaatc cgctaaagaa aaacggtgaa acggctccca tcatcgagga
     1741 agggaaagtg catttggata tttctctggt cattgaacta catatttccg ataagatgct
     1801 atcccatcaa gttgaaaata atttgaattg tgatgaggcc aaaattttct tatccgaatg
     1861 taaaaacctt ctcttaaaac aacgtattgc cggcggttct gtattgagta ttgaagatgt
     1921 tcaattgcac taccttgaag atgagtggaa aattaaaagg caactcctcc ccggctttgt
     1981 gttaatgaat gcgcaagcgg aactgattaa aattacggaa gaattgcaac aaggccttaa
     2041 gcaaggcgat tttgttgtat tagaagccaa ccctgatgca accgccttcg atgccttaat
     2101 cgaaacagca attttgcacc atattccgaa tcatcctcac gccaaaacga acgaatggca
     2161 aacttacagc attaaacaag gaagaggctg gcttgtgccg attccggtgg ggtatcaagc
     2221 catttctccc ctatttgaag caggacaatt acaacattgc cgtacgcagg attatccaag
     2281 tcaatatgtg gaaaccctat atagtttggg taaatgggtt tcaccactca acctgcccga
     2341 agatttatcc cgctgttttt ggcgttatac cgaacctaaa aataatttat acctcatttc
     2401 acaaggagac caataatgag aaaattaacc acaccaaccg tattagcttt tgaacgtaat
     2461 ttagatattt ccgatgctgt ttttacgcaa tttaacagcc aagataaaaa tcccgttgaa
     2521 gaagaagtca agatccgtga aaagtctgtt cgcggaacaa tttctaatcg tcttaaaaat
     2581 acgattacga atgatccgac aaaattagat gctgaaattc aaaaacctaa tttacaacgc
     2641 gttgatgttg ctacattaaa tgataaaaac gatatattgg ttgcccgttt cacctgtaaa
     2701 gttttaccgt ttgatggcaa accttgcgta tgtaacgatc aagactacca aagcaaaatt
     2761 attggcgtcg ttgaaagcta cattaaaaca caaggcttct ccgaattagc caaacgctat
     2821 gccacaaata tcgctaatgc acgctggtta tggcgcaatc gcatgagtgc ggaaacgatc
     2881 aaaattgaag tgtcatttaa cggtgaaacg ctgacttttg ataatgccaa agcgctttcg
     2941 ctcaatactt tcgacgattc ttctacgcaa cttcaggttc ttaccgggca tattgaagac
     3001 ggactgagcg gaaaacattt cgtgatttta aatatcaccg cttatgtcaa aatgggttac
     3061 ggacaagaag tttatccttc ccaagaactc gtgctggatg cgggagctaa taaccgtaaa
     3121 agtaaggtgc tttataagct tgatgatagt ggcaaagcag caatgcactc acaaaaagtc
     3181 agcaatgcca ttcgcaccat tgatacatgg tataaatcaa atgccgagtt tccaattgca
     3241 accgaacctt atggcgcagt aaccacgctc ggtaccgctt tccgccaacc aaaagaaaaa
     3301 ttagattttt atacattgtt cgataattgg ctcctaaacg acaaggtccc tgatgttgaa
     3361 cagcagcatt atgtgatgag cgtgttaatc cgcggcggtg tatttggcgc aagcggtaag
     3421 gaatagccat gacagtgcaa acccattaca ttgaaatcaa ggcgattccg caagtggaca
     3481 tgttacaaac ggaagtcatc ggcttctgtt tgcaaaaact ccaccaaatc ctaccgcact
     3541 ttgaaggtag gattgggctc gcctttcccg cctacggcaa cgacaaaacc ttgggcggca
     3601 ttattcgcct gtttggcacg gaaaatgatt gcggttttat tcattttaaa ctgcaatcac
     3661 tgcgggatta tgcccttatc agcgaagtca tgccaattcc agaaaaagtc agaagttacc
     3721 gcatttatca gcgtattcag cctaagggac aaagttccat tcgacgtgca gaaaaacgcc
     3781 tgacagcaca aggaaaatgg aatgaggaag ttctacaaaa tatgttgcag aaacaagcga
     3841 cacagcgaat ctacccacat gctcatttga aaagtagcag tacaaaacag caatttattt
     3901 tagcgatcaa atccgtgcat caaacaaaag ccgttgaagg ggtgtttagt gcttacggat
     3961 taagtcaaac tacaaccgtt ccacatttct aattaat
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