BBa_K1773023 1 BBa_K1773023 Cascade with pLux/cI right promoter and Medium RBS 2015-08-26T11:00:00Z 2015-08-27T02:08:24Z Cascade complex was cloned form Aggregatibacter actinomycetemcomitans D-7S strain. I-F type Cascade complex of I-F type CRISPR-Cas system. The Cascade complex is comprised of five proteins : Csy1, Csy2, Csy3 and Cas6f and an crRNA molecule. The Cascade complex distinguishes target DNA , and recruits Cas3 (BBa_K1773003) protein, which degrades the target DNA. false false _2195_ 27269 27269 9 false no false Sarunas Tumas component2438483 1 BBa_K1773017 component2438484 1 BBa_K1773005 annotation2438484 1 BBa_K1773005 range2438484 1 100 3997 annotation2438483 1 BBa_K1773017 range2438483 1 1 91 BBa_K1773005 1 BBa_K1773005 I-F type CRISPR-Cas Cascade complex 2015-06-29T11:00:00Z 2015-09-10T12:26:44Z Aggregatibacter actinomycetemcomitans D-7S I-F type CRISPR-Cas Cascade complex mutated for biobrick assembly. false false _2195_ 27269 27269 9 false no false Sarunas Tumas BBa_K1773017 1 BBa_K1773017 pLux/cI right promoter with medium RBS 2015-08-25T11:00:00Z 2015-08-26T02:05:21Z From parts registry pLux/cI right promoter with medium RBS. false false _2195_ 27269 27269 9 false assembled with oligonucleotide primers false Sarunas Tumas component2437997 1 BBa_K1773015 component2437999 1 BBa_B0032 annotation2437997 1 BBa_K1773015 range2437997 1 1 78 annotation2437999 1 BBa_B0032 range2437999 1 79 91 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 BBa_K1773015 1 BBa_K1773015 pLux/ci lambda promoter with 10bp at 3' end for RBS attachment 2015-08-24T11:00:00Z 2015-08-25T05:56:07Z no pLux/ci lambda promoter with 10bp at 3' end for RBS attachment. It is a hipothetical part for assembling pLux/cI+RBS biobricks false false _2195_ 27269 27269 9 false no false Sarunas Tumas BBa_K1773017_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatacctctggcggtgatacacatgcatatcacacaggaaag BBa_B0032_sequence 1 tcacacaggaaag BBa_K1773023_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatacctctggcggtgatacacatgcatatcacacaggaaagtactagagcatgataactaaaacaacagtagtaaaagccattcaacaatttcttgttgatcaaaatctaaagaaatccggaaccgatagaaagaaactagaaaatgcctctctttcagaatcagaaaaagaagcattattgaaaaaaatcgccgagtatgatgaaaaataccgttttgatgtttgggttgaaaatgccgctacggtaatggcaaagcagttgtatttcggtacgcacatttcccgtggaatccactcttcctcaaaaggtgataatgttaattttcaatctgatttgacgctttccgatacacttgtcggttcgcaatcaatacctaagttagaacttgatgcaaacgggaatgcggctgcgcttcccttagccggtttctttaatattattgttgatgaggaaaaaaatacgacacttaaagaattgttacttaatgacgattcttgccttgaaggctgtttcgctgatgatcctgaattatccaagcaatataagatttgctttcaaaatgcattaaaaggggatttaacaaaaccgagaaccgacgagcgtaataaacaactcctatgggcaaataatttcgattctggaaagaatgcttatatttgcctaattcccttgtatccgtcctcattaaccagtcatttttaccataaaattaatcaattgcgttattctgactataacaagcaatcatcatccaatcgctataaaaaagatgtatcgcaacttccttatgtatcattacaaaatataggggtaactgtgttgggcggaacaaagccacagaatgtcagccaacttaccagtaaccaagggggacggaattatttgcttccttcacttccgcctatttttaaatctcaagaaaaaatacgtttaactcaaaaacaaaccaccatttttaatcgtcgtttggcttatgcttgtcgtgaaggattaaatatgctgtatgaagtcgttgaagcagaaaaaaatatttatccggaaagagataaacgtaaattggcgttagagttgattgctcacactgttttaatgcaagcgaagtacattcaaaataactcccctacaggttggagcaaagactatcaccttaatgaatatcaaaaatattggcttgatccacaccgtgcagaactggatggcgaagataattttagacataacattgagcaaaaccattggaccgccgaaattatccgtcaatttgcactttgggttaatgcttgccttaaatggcgttttaaatctattcaacaggattttactacacctgaatataaccaatggtgcagagaaatggaaaaagccattgctgcacaagcccggttaagtcaataaggatcaaatatgaacactgatttctatttacttttcaagcatattaaaatccagtccgccaatgcgatttcaggtcctttaagctatggttttcctgccgttagcggctttgtcggtgcgatacatgcgttaagtcgtaagctaaatgaaaaagccatgttagatggtgtaacaattgcttgccataactatgatttacaggcttaccaatccacaccattttccgaccgcacttttatccagacccgtaatccgctaaagaaaaacggtgaaacggctcccatcatcgaggaagggaaagtgcatttggatatttctctggtcattgaactacatatttccgataagatgctatcccatcaagttgaaaataatttgaattgtgatgaggccaaaattttcttatccgaatgtaaaaaccttctcttaaaacaacgtattgccggcggttctgtattgagtattgaagatgttcaattgcactaccttgaagatgagtggaaaattaaaaggcaactcctccccggctttgtgttaatgaatgcgcaagcggaactgattaaaattacggaagaattgcaacaaggccttaagcaaggcgattttgttgtattagaagccaaccctgatgcaaccgccttcgatgccttaatcgaaacagcaattttgcaccatattccgaatcatcctcacgccaaaacgaacgaatggcaaacttacagcattaaacaaggaagaggctggcttgtgccgattccggtggggtatcaagccatttctcccctatttgaagcaggacaattacaacattgccgtacgcaggattatccaagtcaatatgtggaaaccctatatagtttgggtaaatgggtttcaccactcaacctgcccgaagatttatcccgctgtttttggcgttataccgaacctaaaaataatttatacctcatttcacaaggagaccaataatgagaaaattaaccacaccaaccgtattagcttttgaacgtaatttagatatttccgatgctgtttttacgcaatttaacagccaagataaaaatcccgttgaagaagaagtcaagatccgtgaaaagtctgttcgcggaacaatttctaatcgtcttaaaaatacgattacgaatgatccgacaaaattagatgctgaaattcaaaaacctaatttacaacgcgttgatgttgctacattaaatgataaaaacgatatattggttgcccgtttcacctgtaaagttttaccgtttgatggcaaaccttgcgtatgtaacgatcaagactaccaaagcaaaattattggcgtcgttgaaagctacattaaaacacaaggcttctccgaattagccaaacgctatgccacaaatatcgctaatgcacgctggttatggcgcaatcgcatgagtgcggaaacgatcaaaattgaagtgtcatttaacggtgaaacgctgacttttgataatgccaaagcgctttcgctcaatactttcgacgattcttctacgcaacttcaggttcttaccgggcatattgaagacggactgagcggaaaacatttcgtgattttaaatatcaccgcttatgtcaaaatgggttacggacaagaagtttatccttcccaagaactcgtgctggatgcgggagctaataaccgtaaaagtaaggtgctttataagcttgatgatagtggcaaagcagcaatgcactcacaaaaagtcagcaatgccattcgcaccattgatacatggtataaatcaaatgccgagtttccaattgcaaccgaaccttatggcgcagtaaccacgctcggtaccgctttccgccaaccaaaagaaaaattagatttttatacattgttcgataattggctcctaaacgacaaggtccctgatgttgaacagcagcattatgtgatgagcgtgttaatccgcggcggtgtatttggcgcaagcggtaaggaatagccatgacagtgcaaacccattacattgaaatcaaggcgattccgcaagtggacatgttacaaacggaagtcatcggcttctgtttgcaaaaactccaccaaatcctaccgcactttgaaggtaggattgggctcgcctttcccgcctacggcaacgacaaaaccttgggcggcattattcgcctgtttggcacggaaaatgattgcggttttattcattttaaactgcaatcactgcgggattatgcccttatcagcgaagtcatgccaattccagaaaaagtcagaagttaccgcatttatcagcgtattcagcctaagggacaaagttccattcgacgtgcagaaaaacgcctgacagcacaaggaaaatggaatgaggaagttctacaaaatatgttgcagaaacaagcgacacagcgaatctacccacatgctcatttgaaaagtagcagtacaaaacagcaatttattttagcgatcaaatccgtgcatcaaacaaaagccgttgaaggggtgtttagtgcttacggattaagtcaaactacaaccgttccacatttctaattaat BBa_K1773005_sequence 1 catgataactaaaacaacagtagtaaaagccattcaacaatttcttgttgatcaaaatctaaagaaatccggaaccgatagaaagaaactagaaaatgcctctctttcagaatcagaaaaagaagcattattgaaaaaaatcgccgagtatgatgaaaaataccgttttgatgtttgggttgaaaatgccgctacggtaatggcaaagcagttgtatttcggtacgcacatttcccgtggaatccactcttcctcaaaaggtgataatgttaattttcaatctgatttgacgctttccgatacacttgtcggttcgcaatcaatacctaagttagaacttgatgcaaacgggaatgcggctgcgcttcccttagccggtttctttaatattattgttgatgaggaaaaaaatacgacacttaaagaattgttacttaatgacgattcttgccttgaaggctgtttcgctgatgatcctgaattatccaagcaatataagatttgctttcaaaatgcattaaaaggggatttaacaaaaccgagaaccgacgagcgtaataaacaactcctatgggcaaataatttcgattctggaaagaatgcttatatttgcctaattcccttgtatccgtcctcattaaccagtcatttttaccataaaattaatcaattgcgttattctgactataacaagcaatcatcatccaatcgctataaaaaagatgtatcgcaacttccttatgtatcattacaaaatataggggtaactgtgttgggcggaacaaagccacagaatgtcagccaacttaccagtaaccaagggggacggaattatttgcttccttcacttccgcctatttttaaatctcaagaaaaaatacgtttaactcaaaaacaaaccaccatttttaatcgtcgtttggcttatgcttgtcgtgaaggattaaatatgctgtatgaagtcgttgaagcagaaaaaaatatttatccggaaagagataaacgtaaattggcgttagagttgattgctcacactgttttaatgcaagcgaagtacattcaaaataactcccctacaggttggagcaaagactatcaccttaatgaatatcaaaaatattggcttgatccacaccgtgcagaactggatggcgaagataattttagacataacattgagcaaaaccattggaccgccgaaattatccgtcaatttgcactttgggttaatgcttgccttaaatggcgttttaaatctattcaacaggattttactacacctgaatataaccaatggtgcagagaaatggaaaaagccattgctgcacaagcccggttaagtcaataaggatcaaatatgaacactgatttctatttacttttcaagcatattaaaatccagtccgccaatgcgatttcaggtcctttaagctatggttttcctgccgttagcggctttgtcggtgcgatacatgcgttaagtcgtaagctaaatgaaaaagccatgttagatggtgtaacaattgcttgccataactatgatttacaggcttaccaatccacaccattttccgaccgcacttttatccagacccgtaatccgctaaagaaaaacggtgaaacggctcccatcatcgaggaagggaaagtgcatttggatatttctctggtcattgaactacatatttccgataagatgctatcccatcaagttgaaaataatttgaattgtgatgaggccaaaattttcttatccgaatgtaaaaaccttctcttaaaacaacgtattgccggcggttctgtattgagtattgaagatgttcaattgcactaccttgaagatgagtggaaaattaaaaggcaactcctccccggctttgtgttaatgaatgcgcaagcggaactgattaaaattacggaagaattgcaacaaggccttaagcaaggcgattttgttgtattagaagccaaccctgatgcaaccgccttcgatgccttaatcgaaacagcaattttgcaccatattccgaatcatcctcacgccaaaacgaacgaatggcaaacttacagcattaaacaaggaagaggctggcttgtgccgattccggtggggtatcaagccatttctcccctatttgaagcaggacaattacaacattgccgtacgcaggattatccaagtcaatatgtggaaaccctatatagtttgggtaaatgggtttcaccactcaacctgcccgaagatttatcccgctgtttttggcgttataccgaacctaaaaataatttatacctcatttcacaaggagaccaataatgagaaaattaaccacaccaaccgtattagcttttgaacgtaatttagatatttccgatgctgtttttacgcaatttaacagccaagataaaaatcccgttgaagaagaagtcaagatccgtgaaaagtctgttcgcggaacaatttctaatcgtcttaaaaatacgattacgaatgatccgacaaaattagatgctgaaattcaaaaacctaatttacaacgcgttgatgttgctacattaaatgataaaaacgatatattggttgcccgtttcacctgtaaagttttaccgtttgatggcaaaccttgcgtatgtaacgatcaagactaccaaagcaaaattattggcgtcgttgaaagctacattaaaacacaaggcttctccgaattagccaaacgctatgccacaaatatcgctaatgcacgctggttatggcgcaatcgcatgagtgcggaaacgatcaaaattgaagtgtcatttaacggtgaaacgctgacttttgataatgccaaagcgctttcgctcaatactttcgacgattcttctacgcaacttcaggttcttaccgggcatattgaagacggactgagcggaaaacatttcgtgattttaaatatcaccgcttatgtcaaaatgggttacggacaagaagtttatccttcccaagaactcgtgctggatgcgggagctaataaccgtaaaagtaaggtgctttataagcttgatgatagtggcaaagcagcaatgcactcacaaaaagtcagcaatgccattcgcaccattgatacatggtataaatcaaatgccgagtttccaattgcaaccgaaccttatggcgcagtaaccacgctcggtaccgctttccgccaaccaaaagaaaaattagatttttatacattgttcgataattggctcctaaacgacaaggtccctgatgttgaacagcagcattatgtgatgagcgtgttaatccgcggcggtgtatttggcgcaagcggtaaggaatagccatgacagtgcaaacccattacattgaaatcaaggcgattccgcaagtggacatgttacaaacggaagtcatcggcttctgtttgcaaaaactccaccaaatcctaccgcactttgaaggtaggattgggctcgcctttcccgcctacggcaacgacaaaaccttgggcggcattattcgcctgtttggcacggaaaatgattgcggttttattcattttaaactgcaatcactgcgggattatgcccttatcagcgaagtcatgccaattccagaaaaagtcagaagttaccgcatttatcagcgtattcagcctaagggacaaagttccattcgacgtgcagaaaaacgcctgacagcacaaggaaaatggaatgaggaagttctacaaaatatgttgcagaaacaagcgacacagcgaatctacccacatgctcatttgaaaagtagcagtacaaaacagcaatttattttagcgatcaaatccgtgcatcaaacaaaagccgttgaaggggtgtttagtgcttacggattaagtcaaactacaaccgttccacatttctaattaat BBa_K1773015_sequence 1 acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaatacctctggcggtgatacacatgcata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z