LOCUS BBa_K1773024 3995 bp DNA linear 26-Dec-2024 DEFINITION Cascade with pLux/cI right promoter and weak RBS ACCESSION BBa_K1773024 VERSION BBa_K1773024.1 FEATURES Location/Qualifiers promoter 1..78 /label=BBa_K1773015 RBS 79..89 /label=RBS-4\Weaker RBS 85..88 /label=RBS misc_feature 79..89 /label=BBa_B0033 RBS 79..89 /label=BBa_B0033 promoter 1..89 /label=BBa_K1773018 CDS 98..3995 /label=BBa_K1773005 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atacctctgg 61 cggtgataca catgcatatc acacaggact actagagcat gataactaaa acaacagtag 121 taaaagccat tcaacaattt cttgttgatc aaaatctaaa gaaatccgga accgatagaa 181 agaaactaga aaatgcctct ctttcagaat cagaaaaaga agcattattg aaaaaaatcg 241 ccgagtatga tgaaaaatac cgttttgatg tttgggttga aaatgccgct acggtaatgg 301 caaagcagtt gtatttcggt acgcacattt cccgtggaat ccactcttcc tcaaaaggtg 361 ataatgttaa ttttcaatct gatttgacgc tttccgatac acttgtcggt tcgcaatcaa 421 tacctaagtt agaacttgat gcaaacggga atgcggctgc gcttccctta gccggtttct 481 ttaatattat tgttgatgag gaaaaaaata cgacacttaa agaattgtta cttaatgacg 541 attcttgcct tgaaggctgt ttcgctgatg atcctgaatt atccaagcaa tataagattt 601 gctttcaaaa tgcattaaaa ggggatttaa caaaaccgag aaccgacgag cgtaataaac 661 aactcctatg ggcaaataat ttcgattctg gaaagaatgc ttatatttgc ctaattccct 721 tgtatccgtc ctcattaacc agtcattttt accataaaat taatcaattg cgttattctg 781 actataacaa gcaatcatca tccaatcgct ataaaaaaga tgtatcgcaa cttccttatg 841 tatcattaca aaatataggg gtaactgtgt tgggcggaac aaagccacag aatgtcagcc 901 aacttaccag taaccaaggg ggacggaatt atttgcttcc ttcacttccg cctattttta 961 aatctcaaga aaaaatacgt ttaactcaaa aacaaaccac catttttaat cgtcgtttgg 1021 cttatgcttg tcgtgaagga ttaaatatgc tgtatgaagt cgttgaagca gaaaaaaata 1081 tttatccgga aagagataaa cgtaaattgg cgttagagtt gattgctcac actgttttaa 1141 tgcaagcgaa gtacattcaa aataactccc ctacaggttg gagcaaagac tatcacctta 1201 atgaatatca aaaatattgg cttgatccac accgtgcaga actggatggc gaagataatt 1261 ttagacataa cattgagcaa aaccattgga ccgccgaaat tatccgtcaa tttgcacttt 1321 gggttaatgc ttgccttaaa tggcgtttta aatctattca acaggatttt actacacctg 1381 aatataacca atggtgcaga gaaatggaaa aagccattgc tgcacaagcc cggttaagtc 1441 aataaggatc aaatatgaac actgatttct atttactttt caagcatatt aaaatccagt 1501 ccgccaatgc gatttcaggt cctttaagct atggttttcc tgccgttagc ggctttgtcg 1561 gtgcgataca tgcgttaagt cgtaagctaa atgaaaaagc catgttagat ggtgtaacaa 1621 ttgcttgcca taactatgat ttacaggctt accaatccac accattttcc gaccgcactt 1681 ttatccagac ccgtaatccg ctaaagaaaa acggtgaaac ggctcccatc atcgaggaag 1741 ggaaagtgca tttggatatt tctctggtca ttgaactaca tatttccgat aagatgctat 1801 cccatcaagt tgaaaataat ttgaattgtg atgaggccaa aattttctta tccgaatgta 1861 aaaaccttct cttaaaacaa cgtattgccg gcggttctgt attgagtatt gaagatgttc 1921 aattgcacta ccttgaagat gagtggaaaa ttaaaaggca actcctcccc ggctttgtgt 1981 taatgaatgc gcaagcggaa ctgattaaaa ttacggaaga attgcaacaa ggccttaagc 2041 aaggcgattt tgttgtatta gaagccaacc ctgatgcaac cgccttcgat gccttaatcg 2101 aaacagcaat tttgcaccat attccgaatc atcctcacgc caaaacgaac gaatggcaaa 2161 cttacagcat taaacaagga agaggctggc ttgtgccgat tccggtgggg tatcaagcca 2221 tttctcccct atttgaagca ggacaattac aacattgccg tacgcaggat tatccaagtc 2281 aatatgtgga aaccctatat agtttgggta aatgggtttc accactcaac ctgcccgaag 2341 atttatcccg ctgtttttgg cgttataccg aacctaaaaa taatttatac ctcatttcac 2401 aaggagacca ataatgagaa aattaaccac accaaccgta ttagcttttg aacgtaattt 2461 agatatttcc gatgctgttt ttacgcaatt taacagccaa gataaaaatc ccgttgaaga 2521 agaagtcaag atccgtgaaa agtctgttcg cggaacaatt tctaatcgtc ttaaaaatac 2581 gattacgaat gatccgacaa aattagatgc tgaaattcaa aaacctaatt tacaacgcgt 2641 tgatgttgct acattaaatg ataaaaacga tatattggtt gcccgtttca cctgtaaagt 2701 tttaccgttt gatggcaaac cttgcgtatg taacgatcaa gactaccaaa gcaaaattat 2761 tggcgtcgtt gaaagctaca ttaaaacaca aggcttctcc gaattagcca aacgctatgc 2821 cacaaatatc gctaatgcac gctggttatg gcgcaatcgc atgagtgcgg aaacgatcaa 2881 aattgaagtg tcatttaacg gtgaaacgct gacttttgat aatgccaaag cgctttcgct 2941 caatactttc gacgattctt ctacgcaact tcaggttctt accgggcata ttgaagacgg 3001 actgagcgga aaacatttcg tgattttaaa tatcaccgct tatgtcaaaa tgggttacgg 3061 acaagaagtt tatccttccc aagaactcgt gctggatgcg ggagctaata accgtaaaag 3121 taaggtgctt tataagcttg atgatagtgg caaagcagca atgcactcac aaaaagtcag 3181 caatgccatt cgcaccattg atacatggta taaatcaaat gccgagtttc caattgcaac 3241 cgaaccttat ggcgcagtaa ccacgctcgg taccgctttc cgccaaccaa aagaaaaatt 3301 agatttttat acattgttcg ataattggct cctaaacgac aaggtccctg atgttgaaca 3361 gcagcattat gtgatgagcg tgttaatccg cggcggtgta tttggcgcaa gcggtaagga 3421 atagccatga cagtgcaaac ccattacatt gaaatcaagg cgattccgca agtggacatg 3481 ttacaaacgg aagtcatcgg cttctgtttg caaaaactcc accaaatcct accgcacttt 3541 gaaggtagga ttgggctcgc ctttcccgcc tacggcaacg acaaaacctt gggcggcatt 3601 attcgcctgt ttggcacgga aaatgattgc ggttttattc attttaaact gcaatcactg 3661 cgggattatg cccttatcag cgaagtcatg ccaattccag aaaaagtcag aagttaccgc 3721 atttatcagc gtattcagcc taagggacaa agttccattc gacgtgcaga aaaacgcctg 3781 acagcacaag gaaaatggaa tgaggaagtt ctacaaaata tgttgcagaa acaagcgaca 3841 cagcgaatct acccacatgc tcatttgaaa agtagcagta caaaacagca atttatttta 3901 gcgatcaaat ccgtgcatca aacaaaagcc gttgaagggg tgtttagtgc ttacggatta 3961 agtcaaacta caaccgttcc acatttctaa ttaat //