##gff-version 3
##sequence-region BBa_K1773026 1 2022
BBa_K1773026	.	engineered_region	1149	2022	.	+	0	ID=BBa_K1773025;Name=BBa_K1773025
BBa_K1773026	.	promoter	1149	1216	.	+	0	ID=BBa_K1773012;Name=BBa_K1773012;Parent=BBa_K1773025
BBa_K1773026	.	engineered_region	1225	2022	.	+	0	ID=BBa_K805016;Name=BBa_K805016;Parent=BBa_K1773025
BBa_K1773026	.	ribosome_entry_site	1225	1236	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K805016
BBa_K1773026	.	conserved_region	1229	1232	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1773026	.	CDS	1243	1860	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K805016
BBa_K1773026	.	engineered_region	1243	1860	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1773026	.	tag	1822	1853	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1773026	.	engineered_tag	1861	1885	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1773026	.	CDS	1243	1821	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1773026	.	terminator	1894	2022	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K805016
BBa_K1773026	.	terminator	1894	1973	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1773026	.	stem_loop	1905	1948	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1773026	.	engineered_region	1894	1973	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1773026	.	terminator	1982	2022	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1773026	.	engineered_region	1982	2022	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1773026	.	stop_codon	2015	2015	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1773026	.	stem_loop	1989	2008	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1773026	.	polyA_site	2009	2022	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1773026	.	engineered_region	1	1140	.	+	0	ID=BBa_K1773006;Name=BBa_K1773006
BBa_K1773026	.	engineered_region	209	1140	.	+	0	ID=BBa_P0151;Name=BBa_P0151;Parent=BBa_K1773006
BBa_K1773026	.	ribosome_entry_site	209	222	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_P0151
BBa_K1773026	.	conserved_region	215	218	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K1773026	.	terminator	1100	1140	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0151
BBa_K1773026	.	engineered_region	1100	1140	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1773026	.	stop_codon	1133	1133	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1773026	.	stem_loop	1107	1126	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1773026	.	polyA_site	1127	1140	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1773026	.	CDS	229	978	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_P0151
BBa_K1773026	.	tag	940	972	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K1773026	.	CDS	232	939	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K1773026	.	engineered_tag	979	1003	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K1773026	.	terminator	1012	1091	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0151
BBa_K1773026	.	stem_loop	1023	1066	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1773026	.	engineered_region	1012	1091	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1773026	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K1773006
BBa_K1773026	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1773026	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1773026	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1773026	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1773026	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1773026	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1773026	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
>BBa_K1773026
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaacctactagatgagcaca
aaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaac
ttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaa
tgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgcc
agagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctc
atgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaa
aaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagcttt
cctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtg
atgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgat
cccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaac
gacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggt
ttgttatagtcgaatacctctggcggtgatatactagagaaagaggagaaatactagatgactataatgataaaaaaat
cggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttga
gtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgat
gatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaat
tgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaa
gataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattaca
gaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagaca
aagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtctt
aaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttata