LOCUS BBa_K1774000 7191 bp DNA linear 22-Nov-2024 DEFINITION CRISPR-Cas9 contaiment device repressed by arabinose and tryptophan ACCESSION BBa_K1774000 VERSION BBa_K1774000.1 FEATURES Location/Qualifiers misc_feature 1..1114 /label=BBa_I13458 promoter 1123..1252 /label=BBa_I13453 promoter 1..1252 /label=BBa_K1333315 misc_feature 1265..1268 /label=conserved RBS 1261..1272 /label=BBa_B0034 CDS 1282..1989 /label=cI lambda misc_feature 1990..2022 /label=LVA misc_feature 2029..2053 /label=Help:Barcodes CDS 1279..2028 /label=BBa_C0051 stem_loop 2073..2116 /label=stem_loop misc_feature 2062..2141 /label=BBa_B0010 terminator 2062..2141 /label=BBa_B0010 stem_loop 2157..2176 /label=T7 TE misc_feature 2183..2183 /label=stop polyA_site 2177..2190 /label=polya misc_feature 2150..2190 /label=BBa_B0012 terminator 2150..2190 /label=BBa_B0012 promoter 2236..2241 /label=-10 promoter 2213..2218 /label=-35 misc_feature 2223..2239 /label=OR1 misc_feature 2199..2215 /label=OR2 misc_feature 2199..2247 /label=BBa_R0051 promoter 2199..2247 /label=BBa_R0051 misc_feature 1261..2247 /label=BBa_Q04510 misc_feature 2260..2263 /label=conserved RBS 2256..2267 /label=BBa_B0034 CDS 2274..6380 /label=BBa_K1774001 stem_loop 6400..6443 /label=stem_loop misc_feature 6389..6468 /label=BBa_B0010 terminator 6389..6468 /label=BBa_B0010 stem_loop 6484..6503 /label=T7 TE misc_feature 6510..6510 /label=stop polyA_site 6504..6517 /label=polya misc_feature 6477..6517 /label=BBa_B0012 terminator 6477..6517 /label=BBa_B0012 terminator 6389..6517 /label=BBa_B0015 promoter 6526..6560 /label=BBa_J23106 misc_feature 6573..6576 /label=conserved RBS 6569..6580 /label=BBa_B0034 CDS 6587..6913 /label=trpR CDS 6587..6913 /label=BBa_K588000 stem_loop 6933..6976 /label=stem_loop misc_feature 6922..7001 /label=BBa_B0010 terminator 6922..7001 /label=BBa_B0010 stem_loop 7017..7036 /label=T7 TE misc_feature 7043..7043 /label=stop polyA_site 7037..7050 /label=polya misc_feature 7010..7050 /label=BBa_B0012 terminator 7010..7050 /label=BBa_B0012 terminator 6922..7050 /label=BBa_B0015 promoter 7059..7107 /label=ptrpL misc_feature 7078..7098 /label=ptrpO promoter 7059..7107 /label=BBa_K588001 misc_feature 6569..7107 /label=BBa_K1774003 misc_feature 7116..7191 /label=BBa_K1774002 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctattactag agacattgat tatttgcacg 1141 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac 1201 gctttttatc gcaactctct actgtttctc cataccgttt ttttgggcta gctactagag 1261 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag 1321 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag 1381 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc 1441 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt 1501 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg 1561 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg 1621 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc 1681 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca 1741 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct 1801 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa 1861 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc 1921 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag 1981 acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc 2041 tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 2101 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 2161 caccttcggg tgggcctttc tgcgtttata tactagagta acaccgtgcg tgttgactat 2221 tttacctctg gcggtgataa tggttgctac tagagaaaga ggagaaatac tagatggaca 2281 aaaaatactc aatcggactg gatattggta ctaacagtgt gggctgggcc gtaattaccg 2341 atgaatataa agtgccgagc aaaaaattca aagtgctggg gaacacggac cgtcattcga 2401 ttaaaaaaaa cctgatcggc gcactgctgt tcgattccgg cgaaaccgcc gaagcgacgc 2461 gcctgaagcg caccgcccgg cgccgctata cccgtcgcaa gaaccgtatc tgttacctgc 2521 aagaaatctt cagcaacgaa atggcaaaag ttgatgacag cttctttcat cgcttagagg 2581 aaagcttcct tgtggaagaa gacaaaaaac atgaacgcca tcctatcttt ggcaatattg 2641 tagacgaagt tgcctaccat gaaaagtatc cgacgattta ccatttgcgt aaaaagctgg 2701 tcgatagcac tgataaagcc gacctgcgtc tgatctatct ggcgctggca cacatgatca 2761 aatttcgtgg ccattttttg attgaaggtg atcttaatcc ggacaattct gatgtcgaca 2821 aattgttcat tcagctggta cagacctata accaattgtt tgaagaaaat ccgattaacg 2881 cgagcggtgt tgatgcaaag gccattctgt ccgcccgtct gtcaaagagt cgtcgtcttg 2941 aaaacttgat tgcgcaactg ccgggcgaaa aaaaaaatgg gcttttcggc aatctgatcg 3001 cattgtcgct gggtctgacc cctaacttta agtcgaactt tgatctcgcg gaagacgcca 3061 agttacaact gagtaaagat acttatgatg atgatctgga caacctcctg gctcagattg 3121 gtgatcaata tgcggatctt tttttagctg cgaagaactt gtctgatgcg attttactgt 3181 ccgatattct gcgcgtgaac accgagatca ctaaagcccc gttgagtgcc tcaatgatta 3241 aacgctatga cgaacaccat caggatctga cgctgctcaa agctctggtt cgccagcagt 3301 taccagagaa gtacaaggag atattcttcg atcagtctaa gaacgggtac gcagggtaca 3361 tcgatggtgg tgcatcacag gaagagttct acaagttcat taaaccgatt ctggagaaaa 3421 tggacggaac ggaagaactg ttggtgaaac tgaaccgtga ggatcttctg cgtaaacaac 3481 gcaccttcga taatggcagc atcccgcacc agattcacct gggcgaactg catgcaattc 3541 tccgccgtca ggaggatttc tacccgtttc tgaaagataa ccgtgagaaa attgaaaaaa 3601 ttctcacctt ccgtattccg tactacgtcg gacctctcgc ccgtgggaac agccgtttcg 3661 cgtggatgac ccgtaagtcg gaagaaacaa tcacgccatg gaacttcgaa gaagtggtcg 3721 ataaaggcgc ctccgcacag tccttcattg agcgcatgac caatttcgac aaaaaccttc 3781 ccaacgaaaa agttctgccg aaacactcgc tgctgtatga atactttacg gtgtacaatg 3841 aactgacgaa agttaaatac gtcaccgaag ggatgcgtaa gcccgccttt ctttctggtg 3901 aacagaaaaa agcaattgtg gatctgctgt tcaaaacaaa ccgtaaagtt accgttaagc 3961 aactcaaaga agattatttt aagaagatcg aatgtttcga ttcggttgaa attagcggcg 4021 tcgaagatcg tttcaacgcg tcgctgggca cctatcacga ccttctgaag atcatcaaag 4081 ataaggactt cctggataat gaagagaacg aggatatcct tgaagatatc gtgcttactt 4141 taaccttgtt cgaagaccgc gaaatgatcg aagaacggct caaaacatat gcccatctgt 4201 tcgatgacaa agttatgaaa cagctgaaac gccgccgtta cacgggttgg ggccgtttgt 4261 cgcgtaaact gattaatggc attcgcgaca aacagtcggg gaaaaccatt cttgattttc 4321 tcaaatccga tggttttgcc aatcgcaact ttatgcagct cattcacgac gatagcctga 4381 ccttcaagga agacatccag aaagcgcaag tgagcggcca gggggacagt ctgcacgagc 4441 acatcgcaaa cctggccgga agcccggcga ttaaaaaagg gatcttacaa actgtcaaag 4501 ttgttgatga attagtaaaa gtgatgggtc gccacaaacc ggagaatatc gtaatcgaaa 4561 tggctcgtga aaaccaaacc actcagaaag gtcagaagaa tagccgcgaa cgtatgaaac 4621 gcattgagga aggcatcaaa gagttgggtt cccagatttt aaaagagcat cctgtcgaaa 4681 atactcagct gcaaaatgaa aaattatacc tgtactatct ccagaatggg cgcgatatgt 4741 atgtggacca ggaacttgac attaaccgtt taagtgacta cgatgtggat catattgttc 4801 cgcaatcttt tctcaaagat gattccattg ataacaaagt gttaacccgt tctgacaaaa 4861 atcgcgggaa atccgacaat gtaccgagcg aggaagtggt caagaagatg aagaactatt 4921 ggcggcagct gctgaacgct aaactgatca cccagcgtaa atttgacaac ctgaccaaag 4981 cagaacgtgg tggactgagt gaattggaca aagcaggttt tattaaacgg cagctggtgg 5041 aaacgcgcca gattactaag cacgtcgcgc aaattctgga tagccgtatg aatacaaagt 5101 acgacgagaa cgataagctc atccgcgaag taaaagtcat taccctgaaa tctaaactgg 5161 tgagtgactt ccgcaaagat tttcagtttt acaaggtccg ggaaattaat aactaccatc 5221 atgcccatga tgcgtacctg aatgcagtag tcggtacagc gttgattaaa aaatatccga 5281 aactggagag tgagttcgtt tatggggact acaaagttta tgacgttcgt aaaatgattg 5341 cgaaaagtga gcaggaaatt ggcaaagcga cggcgaaata cttcttttat agtaacatta 5401 tgaatttctt taagaccgaa atcacattag ccaatggaga aatccgtaag cgtccgctca 5461 tcgagacgaa cggagaaacc ggagaaattg tgtgggacaa aggtcgcgac ttcgccacag 5521 tgcgtaaagt tttatctatg ccacaggtta acatcgtgaa aaaaacggaa gtccaaacgg 5581 gtggcttttc taaagaaagc attttgccca aacgcaacag tgataaattg attgcgcgga 5641 aaaaggattg ggaccctaaa aaatatggcg gctttgattc tccaacagtg gcctattcgg 5701 tgcttgtcgt ggctaaagtg gagaagggaa aatccaaaaa gctgaaaagt gtaaaagaat 5761 tgctggggat cacgatcatg gaacgctctt cgttcgaaaa aaatccgatc gactttttag 5821 aagcaaaagg ttataaagag gtgaagaaag acctgatcat taaattaccg aaatatagct 5881 tgttcgagct ggaaaatggt cgcaaacgta tgttggcctc agcgggagaa ttacaaaaag 5941 gcaacgaact cgcgcttccg agcaaatatg tcaatttcct ttatctggca tcccattatg 6001 agaagctgaa gggcagcccg gaagataatg aacaaaaaca gctgtttgtc gaacagcaca 6061 agcattatct cgatgaaatc attgaacaaa ttagcgaatt ttcaaaacgg gtcatcctcg 6121 cagatgcaaa ccttgacaaa gttctgagcg cgtataataa gcatcgggat aaaccaattc 6181 gcgaacaagc ggaaaacatc attcatttat ttacactgac caacctgggc gcaccggcag 6241 cgtttaaata tttcgatacg acaattgacc gtaaacgcta cacctccacg aaagaagtgt 6301 tggacgccac cctgattcac cagagcatca caggtctgta cgaaacgcgt attgatctga 6361 gtcagctggg aggcgactaa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 6421 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 6481 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtttac ggctagctca 6541 gtcctaggta tagtgctagc tactagagaa agaggagaaa tactagatgg cccaacaatc 6601 accctattca gcagcgatgg cagaacagcg tcaccaggag tggttacgtt ttgtcgacct 6661 gcttaagaat gcctaccaaa acgatctcca tttaccgttg ttaaacctga tgctgacgcc 6721 agatgagcgc gaagcgttgg ggactcgcgt gcgtattgtc gaagagctgt tgcgcggcga 6781 aatgagccag cgtgagttaa aaaatgaact cggcgcaggc atcgcgacga ttacgcgtgg 6841 atctaacagc ctgaaagccg cgcccgtcga gctgcgccag tggctggaag aggtgttgct 6901 gaaaagcgat tgatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 6961 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 7021 caccttcggg tgggcctttc tgcgtttata tactagaggc tgttgacaat taatcatcga 7081 actagttaac tagtacgcaa gttcacgtac tagaggtttt agagctagaa atagcaagtt 7141 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg c //