BBa_K588000 1 BBa_K588000 trpR 2011-09-25T11:00:00Z 2015-05-08T01:12:47Z Cloned from plasmid pJPR2 described in Paluh and Yanofsky NAR 1986 E. coli tryptophan repressor false true _759_ 0 9140 9 In stock false Added RFC10 biobrick prefix and suffix false Kevin Lu annotation2142452 1 trpR range2142452 1 1 327 BBa_I13453 1 BBa_I13453 Pbad promoter 2005-05-24T11:00:00Z 2015-08-31T04:07:34Z Released HQ 2013 PBad promoter from I0500 without AraC. false false _11_ 0 253 6 In stock false true jkm BBa_K1774000 1 BBa_K1774000 CRISPR-Cas9 contaiment device repressed by arabinose and tryptophan 2015-09-17T11:00:00Z 2015-09-20T02:01:34Z Cas9 originated from S. pyogenes SF 370 This is a prototype containment device utilizing CRISPR-Cas9 system which aims to control accidental leakage of GMM once it escape from medium containing arabinose and tryptophan. The device consists of two modules, firstly a arabinose repressible cas9 expression module and a tryptophan repressible gRNA expression module. Under the presence of sufficient amount of arabinose and tryptophan the device remain silent, however when bacteria escape to environment without arabinose and tryptophan, Cas9 and a gRNA targeting DNA polymerase III alpha subunit will be expressed. Subsequent cleavage of the targeted sequence may hinder DNA replication and false false _2196_ 25503 25503 9 false a lot false HKU iGEM 2015 component2474101 1 BBa_K1774003 component2474057 1 BBa_K1333315 component2474075 1 BBa_Q04510 component2474086 1 BBa_J23106 component2474085 1 BBa_B0015 component2474102 1 BBa_K1774002 component2474077 1 BBa_B0034 component2474078 1 BBa_K1774001 annotation2474101 1 BBa_K1774003 range2474101 1 6569 7107 annotation2474086 1 BBa_J23106 range2474086 1 6526 6560 annotation2474102 1 BBa_K1774002 range2474102 1 7116 7191 annotation2474078 1 BBa_K1774001 range2474078 1 2274 6380 annotation2474077 1 BBa_B0034 range2474077 1 2256 2267 annotation2474057 1 BBa_K1333315 range2474057 1 1 1252 annotation2474075 1 BBa_Q04510 range2474075 1 1261 2247 annotation2474085 1 BBa_B0015 range2474085 1 6389 6517 BBa_K1333315 1 BBa_K1333315 pBAD with araC 2014-09-23T11:00:00Z 2015-05-08T01:09:58Z The two parts are all from previous parts. A araBAD promoter with araC transcripted from the negative direction. false false _1708_ 0 15896 9 In stock false We generate this regulatory part to regulate our reporter gene expression in our project. false Wei Tang component2386113 1 BBa_I13453 component2386112 1 BBa_I13458 annotation2386112 1 BBa_I13458 range2386112 1 1 1114 annotation2386113 1 BBa_I13453 range2386113 1 1123 1252 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_R0051 1 cI lam promoter (lambda cI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z <a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Released HQ 2013 The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).</P> false true _1_ 0 24 7 In stock false <P> <P>In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (<b><font color="red">More details needed here! DE, 2/24/03</font></b>)<P> Incompatible with host expressing cI repressor. true Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross annotation2023 1 -35 range2023 1 15 20 annotation2022 1 -10 range2022 1 38 43 annotation2024 1 OR1 range2024 1 25 41 annotation2025 1 OR2 range2025 1 1 17 annotation7067 1 BBa_R0051 range7067 1 1 49 BBa_K1774002 1 BBa_K1774002 gRNA for cas9 targeting DNA polyerase III alpha subunit 2015-09-17T11:00:00Z 2015-09-20T02:02:24Z Generated from sequence of DNA polyerase III alpha subunit in E. coli BL21 DE3 gRNA targeting DNA polyerase III alpha subunit in E. coli BL21 DE3 false false _2196_ 25503 25503 9 false No false HKU iGEM 2015 BBa_K588001 1 BBa_K588001 ptrp 2011-09-25T11:00:00Z 2015-05-08T01:12:47Z Synthesized by IDT Released HQ 2013 Tryptophan promoter and operator false false _759_ 0 9140 9 In stock false The sequence contains SpeI sites at positions critical for function. We modified the SpeI site in the biobrick suffix to an NheI site as done by UNAM 2010 false Kevin Lu annotation2142514 1 ptrpL range2142514 1 1 49 annotation2142550 1 ptrpO range2142550 1 20 40 BBa_K1774001 1 BBa_K1774001 Cas9 (optimized for expression in E. coli) 2015-09-17T11:00:00Z 2015-09-20T02:01:59Z S. pyogenes SF 370 This part code for cas9 originated from S. pyogenes SF 370 with codon optimized for expression in E. coli. false false _2196_ 25503 25503 9 false Presence of illegal RE sites false HKU iGEM 2015 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_C0051 1 cI lam cI repressor from E. coli phage lambda (+LVA) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Released HQ 2013 Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).</P> false false _1_ 0 24 7 In stock false References (unparsed) here: <p><a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000</P> <P><a href="http://www.genesdev.org/cgi/content/full/15/22/3013">Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. </a>Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001</P> <p></p> <P> References (unparsed) here: <p><a href="http://www.nature.com/cgi-taf/DynaPage.taf?file=/nature/journal/v403/n6767/abs/403335a0_fs.html&dynoptions=doi1043774228">A synthetic oscillatory network of transcriptional regulators</a> , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000</P> <P><a href="http://www.genesdev.org/cgi/content/full/15/22/3013">Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. </a>Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001</P> <p></p> <P>BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.<P> true Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross annotation2213991 1 Help:Barcodes range2213991 1 751 775 annotation23334 1 cI lambda range23334 1 4 711 annotation23335 1 LVA range23335 1 712 744 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1774003 1 BBa_K1774003 trpR-pTrp inverter (RBS-trpR-TT-pTrp) 2015-09-17T11:00:00Z 2015-09-20T02:02:42Z Modified from Trp operon Addition of Trp repress anything downstream of the inverter. Notice that it contain illegal SpeI sites, however it it essential for the functioning of this part. false false _2196_ 25503 25503 9 false Two illegal sites false HKU iGEM 2015 component2473996 1 BBa_K588000 component2473994 1 BBa_B0034 component2474003 1 BBa_B0015 component2474006 1 BBa_K588001 annotation2473994 1 BBa_B0034 range2473994 1 1 12 annotation2474003 1 BBa_B0015 range2474003 1 354 482 annotation2473996 1 BBa_K588000 range2473996 1 19 345 annotation2474006 1 BBa_K588001 range2474006 1 491 539 BBa_I13458 1 BBa_I13458 promoter C and arabinose C operon 2005-08-16T11:00:00Z 2015-08-31T04:07:34Z Released HQ 2013 PC and AraC from the AraBAD operator. PC and AraC are on the complementary strand, reading right to left. false false _11_ 0 253 6 In stock false true Josh Michener BBa_Q04510 1 BBa_Q04510 QPI (B0034.C0051.B0015.R0051) 2003-12-02T12:00:00Z 2015-05-08T01:14:14Z Released HQ 2013 Lambda cI QPI w/ strong RBS false true _1_ 0 24 7 In stock false true Caitlin Conboy and Jennifer Braff component952885 1 BBa_B0034 component952911 1 BBa_B0012 component952901 1 BBa_B0010 component952895 1 BBa_C0051 component952926 1 BBa_R0051 annotation952926 1 BBa_R0051 range952926 1 939 987 annotation952895 1 BBa_C0051 range952895 1 19 768 annotation952911 1 BBa_B0012 range952911 1 890 930 annotation952901 1 BBa_B0010 range952901 1 802 881 annotation952885 1 BBa_B0034 range952885 1 1 12 BBa_J23106 1 BBa_J23106 constitutive promoter family member 2006-08-13T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_I13453_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc BBa_K588001_sequence 1 gctgttgacaattaatcatcgaactagttaactagtacgcaagttcacg BBa_B0034_sequence 1 aaagaggagaaa BBa_I13458_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctat BBa_R0051_sequence 1 taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc BBa_K1774001_sequence 1 atggacaaaaaatactcaatcggactggatattggtactaacagtgtgggctgggccgtaattaccgatgaatataaagtgccgagcaaaaaattcaaagtgctggggaacacggaccgtcattcgattaaaaaaaacctgatcggcgcactgctgttcgattccggcgaaaccgccgaagcgacgcgcctgaagcgcaccgcccggcgccgctatacccgtcgcaagaaccgtatctgttacctgcaagaaatcttcagcaacgaaatggcaaaagttgatgacagcttctttcatcgcttagaggaaagcttccttgtggaagaagacaaaaaacatgaacgccatcctatctttggcaatattgtagacgaagttgcctaccatgaaaagtatccgacgatttaccatttgcgtaaaaagctggtcgatagcactgataaagccgacctgcgtctgatctatctggcgctggcacacatgatcaaatttcgtggccattttttgattgaaggtgatcttaatccggacaattctgatgtcgacaaattgttcattcagctggtacagacctataaccaattgtttgaagaaaatccgattaacgcgagcggtgttgatgcaaaggccattctgtccgcccgtctgtcaaagagtcgtcgtcttgaaaacttgattgcgcaactgccgggcgaaaaaaaaaatgggcttttcggcaatctgatcgcattgtcgctgggtctgacccctaactttaagtcgaactttgatctcgcggaagacgccaagttacaactgagtaaagatacttatgatgatgatctggacaacctcctggctcagattggtgatcaatatgcggatctttttttagctgcgaagaacttgtctgatgcgattttactgtccgatattctgcgcgtgaacaccgagatcactaaagccccgttgagtgcctcaatgattaaacgctatgacgaacaccatcaggatctgacgctgctcaaagctctggttcgccagcagttaccagagaagtacaaggagatattcttcgatcagtctaagaacgggtacgcagggtacatcgatggtggtgcatcacaggaagagttctacaagttcattaaaccgattctggagaaaatggacggaacggaagaactgttggtgaaactgaaccgtgaggatcttctgcgtaaacaacgcaccttcgataatggcagcatcccgcaccagattcacctgggcgaactgcatgcaattctccgccgtcaggaggatttctacccgtttctgaaagataaccgtgagaaaattgaaaaaattctcaccttccgtattccgtactacgtcggacctctcgcccgtgggaacagccgtttcgcgtggatgacccgtaagtcggaagaaacaatcacgccatggaacttcgaagaagtggtcgataaaggcgcctccgcacagtccttcattgagcgcatgaccaatttcgacaaaaaccttcccaacgaaaaagttctgccgaaacactcgctgctgtatgaatactttacggtgtacaatgaactgacgaaagttaaatacgtcaccgaagggatgcgtaagcccgcctttctttctggtgaacagaaaaaagcaattgtggatctgctgttcaaaacaaaccgtaaagttaccgttaagcaactcaaagaagattattttaagaagatcgaatgtttcgattcggttgaaattagcggcgtcgaagatcgtttcaacgcgtcgctgggcacctatcacgaccttctgaagatcatcaaagataaggacttcctggataatgaagagaacgaggatatccttgaagatatcgtgcttactttaaccttgttcgaagaccgcgaaatgatcgaagaacggctcaaaacatatgcccatctgttcgatgacaaagttatgaaacagctgaaacgccgccgttacacgggttggggccgtttgtcgcgtaaactgattaatggcattcgcgacaaacagtcggggaaaaccattcttgattttctcaaatccgatggttttgccaatcgcaactttatgcagctcattcacgacgatagcctgaccttcaaggaagacatccagaaagcgcaagtgagcggccagggggacagtctgcacgagcacatcgcaaacctggccggaagcccggcgattaaaaaagggatcttacaaactgtcaaagttgttgatgaattagtaaaagtgatgggtcgccacaaaccggagaatatcgtaatcgaaatggctcgtgaaaaccaaaccactcagaaaggtcagaagaatagccgcgaacgtatgaaacgcattgaggaaggcatcaaagagttgggttcccagattttaaaagagcatcctgtcgaaaatactcagctgcaaaatgaaaaattatacctgtactatctccagaatgggcgcgatatgtatgtggaccaggaacttgacattaaccgtttaagtgactacgatgtggatcatattgttccgcaatcttttctcaaagatgattccattgataacaaagtgttaacccgttctgacaaaaatcgcgggaaatccgacaatgtaccgagcgaggaagtggtcaagaagatgaagaactattggcggcagctgctgaacgctaaactgatcacccagcgtaaatttgacaacctgaccaaagcagaacgtggtggactgagtgaattggacaaagcaggttttattaaacggcagctggtggaaacgcgccagattactaagcacgtcgcgcaaattctggatagccgtatgaatacaaagtacgacgagaacgataagctcatccgcgaagtaaaagtcattaccctgaaatctaaactggtgagtgacttccgcaaagattttcagttttacaaggtccgggaaattaataactaccatcatgcccatgatgcgtacctgaatgcagtagtcggtacagcgttgattaaaaaatatccgaaactggagagtgagttcgtttatggggactacaaagtttatgacgttcgtaaaatgattgcgaaaagtgagcaggaaattggcaaagcgacggcgaaatacttcttttatagtaacattatgaatttctttaagaccgaaatcacattagccaatggagaaatccgtaagcgtccgctcatcgagacgaacggagaaaccggagaaattgtgtgggacaaaggtcgcgacttcgccacagtgcgtaaagttttatctatgccacaggttaacatcgtgaaaaaaacggaagtccaaacgggtggcttttctaaagaaagcattttgcccaaacgcaacagtgataaattgattgcgcggaaaaaggattgggaccctaaaaaatatggcggctttgattctccaacagtggcctattcggtgcttgtcgtggctaaagtggagaagggaaaatccaaaaagctgaaaagtgtaaaagaattgctggggatcacgatcatggaacgctcttcgttcgaaaaaaatccgatcgactttttagaagcaaaaggttataaagaggtgaagaaagacctgatcattaaattaccgaaatatagcttgttcgagctggaaaatggtcgcaaacgtatgttggcctcagcgggagaattacaaaaaggcaacgaactcgcgcttccgagcaaatatgtcaatttcctttatctggcatcccattatgagaagctgaagggcagcccggaagataatgaacaaaaacagctgtttgtcgaacagcacaagcattatctcgatgaaatcattgaacaaattagcgaattttcaaaacgggtcatcctcgcagatgcaaaccttgacaaagttctgagcgcgtataataagcatcgggataaaccaattcgcgaacaagcggaaaacatcattcatttatttacactgaccaacctgggcgcaccggcagcgtttaaatatttcgatacgacaattgaccgtaaacgctacacctccacgaaagaagtgttggacgccaccctgattcaccagagcatcacaggtctgtacgaaacgcgtattgatctgagtcagctgggaggcgactaa BBa_K1333315_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctattactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc BBa_K1774002_sequence 1 gttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_C0051_sequence 1 atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc BBa_Q04510_sequence 1 aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgc BBa_K588000_sequence 1 atggcccaacaatcaccctattcagcagcgatggcagaacagcgtcaccaggagtggttacgttttgtcgacctgcttaagaatgcctaccaaaacgatctccatttaccgttgttaaacctgatgctgacgccagatgagcgcgaagcgttggggactcgcgtgcgtattgtcgaagagctgttgcgcggcgaaatgagccagcgtgagttaaaaaatgaactcggcgcaggcatcgcgacgattacgcgtggatctaacagcctgaaagccgcgcccgtcgagctgcgccagtggctggaagaggtgttgctgaaaagcgattga BBa_K1774003_sequence 1 aaagaggagaaatactagatggcccaacaatcaccctattcagcagcgatggcagaacagcgtcaccaggagtggttacgttttgtcgacctgcttaagaatgcctaccaaaacgatctccatttaccgttgttaaacctgatgctgacgccagatgagcgcgaagcgttggggactcgcgtgcgtattgtcgaagagctgttgcgcggcgaaatgagccagcgtgagttaaaaaatgaactcggcgcaggcatcgcgacgattacgcgtggatctaacagcctgaaagccgcgcccgtcgagctgcgccagtggctggaagaggtgttgctgaaaagcgattgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggctgttgacaattaatcatcgaactagttaactagtacgcaagttcacg BBa_J23106_sequence 1 tttacggctagctcagtcctaggtatagtgctagc BBa_K1774000_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctattactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggacaaaaaatactcaatcggactggatattggtactaacagtgtgggctgggccgtaattaccgatgaatataaagtgccgagcaaaaaattcaaagtgctggggaacacggaccgtcattcgattaaaaaaaacctgatcggcgcactgctgttcgattccggcgaaaccgccgaagcgacgcgcctgaagcgcaccgcccggcgccgctatacccgtcgcaagaaccgtatctgttacctgcaagaaatcttcagcaacgaaatggcaaaagttgatgacagcttctttcatcgcttagaggaaagcttccttgtggaagaagacaaaaaacatgaacgccatcctatctttggcaatattgtagacgaagttgcctaccatgaaaagtatccgacgatttaccatttgcgtaaaaagctggtcgatagcactgataaagccgacctgcgtctgatctatctggcgctggcacacatgatcaaatttcgtggccattttttgattgaaggtgatcttaatccggacaattctgatgtcgacaaattgttcattcagctggtacagacctataaccaattgtttgaagaaaatccgattaacgcgagcggtgttgatgcaaaggccattctgtccgcccgtctgtcaaagagtcgtcgtcttgaaaacttgattgcgcaactgccgggcgaaaaaaaaaatgggcttttcggcaatctgatcgcattgtcgctgggtctgacccctaactttaagtcgaactttgatctcgcggaagacgccaagttacaactgagtaaagatacttatgatgatgatctggacaacctcctggctcagattggtgatcaatatgcggatctttttttagctgcgaagaacttgtctgatgcgattttactgtccgatattctgcgcgtgaacaccgagatcactaaagccccgttgagtgcctcaatgattaaacgctatgacgaacaccatcaggatctgacgctgctcaaagctctggttcgccagcagttaccagagaagtacaaggagatattcttcgatcagtctaagaacgggtacgcagggtacatcgatggtggtgcatcacaggaagagttctacaagttcattaaaccgattctggagaaaatggacggaacggaagaactgttggtgaaactgaaccgtgaggatcttctgcgtaaacaacgcaccttcgataatggcagcatcccgcaccagattcacctgggcgaactgcatgcaattctccgccgtcaggaggatttctacccgtttctgaaagataaccgtgagaaaattgaaaaaattctcaccttccgtattccgtactacgtcggacctctcgcccgtgggaacagccgtttcgcgtggatgacccgtaagtcggaagaaacaatcacgccatggaacttcgaagaagtggtcgataaaggcgcctccgcacagtccttcattgagcgcatgaccaatttcgacaaaaaccttcccaacgaaaaagttctgccgaaacactcgctgctgtatgaatactttacggtgtacaatgaactgacgaaagttaaatacgtcaccgaagggatgcgtaagcccgcctttctttctggtgaacagaaaaaagcaattgtggatctgctgttcaaaacaaaccgtaaagttaccgttaagcaactcaaagaagattattttaagaagatcgaatgtttcgattcggttgaaattagcggcgtcgaagatcgtttcaacgcgtcgctgggcacctatcacgaccttctgaagatcatcaaagataaggacttcctggataatgaagagaacgaggatatccttgaagatatcgtgcttactttaaccttgttcgaagaccgcgaaatgatcgaagaacggctcaaaacatatgcccatctgttcgatgacaaagttatgaaacagctgaaacgccgccgttacacgggttggggccgtttgtcgcgtaaactgattaatggcattcgcgacaaacagtcggggaaaaccattcttgattttctcaaatccgatggttttgccaatcgcaactttatgcagctcattcacgacgatagcctgaccttcaaggaagacatccagaaagcgcaagtgagcggccagggggacagtctgcacgagcacatcgcaaacctggccggaagcccggcgattaaaaaagggatcttacaaactgtcaaagttgttgatgaattagtaaaagtgatgggtcgccacaaaccggagaatatcgtaatcgaaatggctcgtgaaaaccaaaccactcagaaaggtcagaagaatagccgcgaacgtatgaaacgcattgaggaaggcatcaaagagttgggttcccagattttaaaagagcatcctgtcgaaaatactcagctgcaaaatgaaaaattatacctgtactatctccagaatgggcgcgatatgtatgtggaccaggaacttgacattaaccgtttaagtgactacgatgtggatcatattgttccgcaatcttttctcaaagatgattccattgataacaaagtgttaacccgttctgacaaaaatcgcgggaaatccgacaatgtaccgagcgaggaagtggtcaagaagatgaagaactattggcggcagctgctgaacgctaaactgatcacccagcgtaaatttgacaacctgaccaaagcagaacgtggtggactgagtgaattggacaaagcaggttttattaaacggcagctggtggaaacgcgccagattactaagcacgtcgcgcaaattctggatagccgtatgaatacaaagtacgacgagaacgataagctcatccgcgaagtaaaagtcattaccctgaaatctaaactggtgagtgacttccgcaaagattttcagttttacaaggtccgggaaattaataactaccatcatgcccatgatgcgtacctgaatgcagtagtcggtacagcgttgattaaaaaatatccgaaactggagagtgagttcgtttatggggactacaaagtttatgacgttcgtaaaatgattgcgaaaagtgagcaggaaattggcaaagcgacggcgaaatacttcttttatagtaacattatgaatttctttaagaccgaaatcacattagccaatggagaaatccgtaagcgtccgctcatcgagacgaacggagaaaccggagaaattgtgtgggacaaaggtcgcgacttcgccacagtgcgtaaagttttatctatgccacaggttaacatcgtgaaaaaaacggaagtccaaacgggtggcttttctaaagaaagcattttgcccaaacgcaacagtgataaattgattgcgcggaaaaaggattgggaccctaaaaaatatggcggctttgattctccaacagtggcctattcggtgcttgtcgtggctaaagtggagaagggaaaatccaaaaagctgaaaagtgtaaaagaattgctggggatcacgatcatggaacgctcttcgttcgaaaaaaatccgatcgactttttagaagcaaaaggttataaagaggtgaagaaagacctgatcattaaattaccgaaatatagcttgttcgagctggaaaatggtcgcaaacgtatgttggcctcagcgggagaattacaaaaaggcaacgaactcgcgcttccgagcaaatatgtcaatttcctttatctggcatcccattatgagaagctgaagggcagcccggaagataatgaacaaaaacagctgtttgtcgaacagcacaagcattatctcgatgaaatcattgaacaaattagcgaattttcaaaacgggtcatcctcgcagatgcaaaccttgacaaagttctgagcgcgtataataagcatcgggataaaccaattcgcgaacaagcggaaaacatcattcatttatttacactgaccaacctgggcgcaccggcagcgtttaaatatttcgatacgacaattgaccgtaaacgctacacctccacgaaagaagtgttggacgccaccctgattcaccagagcatcacaggtctgtacgaaacgcgtattgatctgagtcagctgggaggcgactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatggcccaacaatcaccctattcagcagcgatggcagaacagcgtcaccaggagtggttacgttttgtcgacctgcttaagaatgcctaccaaaacgatctccatttaccgttgttaaacctgatgctgacgccagatgagcgcgaagcgttggggactcgcgtgcgtattgtcgaagagctgttgcgcggcgaaatgagccagcgtgagttaaaaaatgaactcggcgcaggcatcgcgacgattacgcgtggatctaacagcctgaaagccgcgcccgtcgagctgcgccagtggctggaagaggtgttgctgaaaagcgattgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggctgttgacaattaatcatcgaactagttaactagtacgcaagttcacgtactagaggttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z