##gff-version 3
##sequence-region BBa_K1774000 1 7191
BBa_K1774000	.	ribosome_entry_site	2256	2267	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1774000	.	conserved_region	2260	2263	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1774000	.	CDS	2274	6380	.	+	0	ID=BBa_K1774001;Name=BBa_K1774001
BBa_K1774000	.	mature_transcript_region	7116	7191	.	+	0	ID=BBa_K1774002;Name=BBa_K1774002
BBa_K1774000	.	promoter	1	1252	.	+	0	ID=BBa_K1333315;Name=BBa_K1333315
BBa_K1774000	.	promoter	1123	1252	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K1333315
BBa_K1774000	.	engineered_region	1	1114	.	+	0	ID=BBa_I13458;Name=BBa_I13458;Parent=BBa_K1333315
BBa_K1774000	.	engineered_region	1261	2247	.	+	0	ID=BBa_Q04510;Name=BBa_Q04510
BBa_K1774000	.	ribosome_entry_site	1261	1272	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04510
BBa_K1774000	.	conserved_region	1265	1268	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1774000	.	terminator	2062	2141	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04510
BBa_K1774000	.	engineered_region	2062	2141	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1774000	.	stem_loop	2073	2116	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1774000	.	promoter	2199	2247	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_Q04510
BBa_K1774000	.	engineered_region	2199	2247	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K1774000	.	promoter	2236	2241	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K1774000	.	non_covalent_binding_site	2223	2239	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K1774000	.	promoter	2213	2218	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K1774000	.	non_covalent_binding_site	2199	2215	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K1774000	.	CDS	1279	2028	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_Q04510
BBa_K1774000	.	engineered_tag	2029	2053	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K1774000	.	CDS	1282	1989	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K1774000	.	tag	1990	2022	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K1774000	.	terminator	2150	2190	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04510
BBa_K1774000	.	stop_codon	2183	2183	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1774000	.	polyA_site	2177	2190	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1774000	.	stem_loop	2157	2176	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1774000	.	engineered_region	2150	2190	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1774000	.	terminator	6389	6517	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1774000	.	terminator	6389	6468	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1774000	.	engineered_region	6389	6468	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1774000	.	stem_loop	6400	6443	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1774000	.	terminator	6477	6517	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1774000	.	stop_codon	6510	6510	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1774000	.	polyA_site	6504	6517	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1774000	.	stem_loop	6484	6503	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1774000	.	engineered_region	6477	6517	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1774000	.	promoter	6526	6560	.	+	0	ID=BBa_J23106;Name=BBa_J23106
BBa_K1774000	.	engineered_region	6569	7107	.	+	0	ID=BBa_K1774003;Name=BBa_K1774003
BBa_K1774000	.	CDS	6587	6913	.	+	0	ID=BBa_K588000;Name=BBa_K588000;Parent=BBa_K1774003
BBa_K1774000	.	CDS	6587	6913	.	+	0	ID=annotation2142452;Name=trpR;Parent=BBa_K588000
BBa_K1774000	.	ribosome_entry_site	6569	6580	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1774003
BBa_K1774000	.	conserved_region	6573	6576	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1774000	.	promoter	7059	7107	.	+	0	ID=BBa_K588001;Name=BBa_K588001;Parent=BBa_K1774003
BBa_K1774000	.	promoter	7059	7107	.	+	0	ID=annotation2142514;Name=ptrpL;Parent=BBa_K588001
BBa_K1774000	.	operator	7078	7098	.	+	0	ID=annotation2142550;Name=ptrpO;Parent=BBa_K588001
BBa_K1774000	.	terminator	6922	7050	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1774003
BBa_K1774000	.	terminator	6922	7001	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1774000	.	engineered_region	6922	7001	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1774000	.	stem_loop	6933	6976	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1774000	.	terminator	7010	7050	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1774000	.	stop_codon	7043	7043	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1774000	.	polyA_site	7037	7050	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1774000	.	stem_loop	7017	7036	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1774000	.	engineered_region	7010	7050	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K1774000
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctattactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattag
cggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaag
aggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaat
ttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgtt
ggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttg
aagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtga
gtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcg
gagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcac
caacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgattt
ctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaacca
ctaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctg
aagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgct
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtg
ttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggacaaaaaatactca
atcggactggatattggtactaacagtgtgggctgggccgtaattaccgatgaatataaagtgccgagcaaaaaattca
aagtgctggggaacacggaccgtcattcgattaaaaaaaacctgatcggcgcactgctgttcgattccggcgaaaccgc
cgaagcgacgcgcctgaagcgcaccgcccggcgccgctatacccgtcgcaagaaccgtatctgttacctgcaagaaatc
ttcagcaacgaaatggcaaaagttgatgacagcttctttcatcgcttagaggaaagcttccttgtggaagaagacaaaa
aacatgaacgccatcctatctttggcaatattgtagacgaagttgcctaccatgaaaagtatccgacgatttaccattt
gcgtaaaaagctggtcgatagcactgataaagccgacctgcgtctgatctatctggcgctggcacacatgatcaaattt
cgtggccattttttgattgaaggtgatcttaatccggacaattctgatgtcgacaaattgttcattcagctggtacaga
cctataaccaattgtttgaagaaaatccgattaacgcgagcggtgttgatgcaaaggccattctgtccgcccgtctgtc
aaagagtcgtcgtcttgaaaacttgattgcgcaactgccgggcgaaaaaaaaaatgggcttttcggcaatctgatcgca
ttgtcgctgggtctgacccctaactttaagtcgaactttgatctcgcggaagacgccaagttacaactgagtaaagata
cttatgatgatgatctggacaacctcctggctcagattggtgatcaatatgcggatctttttttagctgcgaagaactt
gtctgatgcgattttactgtccgatattctgcgcgtgaacaccgagatcactaaagccccgttgagtgcctcaatgatt
aaacgctatgacgaacaccatcaggatctgacgctgctcaaagctctggttcgccagcagttaccagagaagtacaagg
agatattcttcgatcagtctaagaacgggtacgcagggtacatcgatggtggtgcatcacaggaagagttctacaagtt
cattaaaccgattctggagaaaatggacggaacggaagaactgttggtgaaactgaaccgtgaggatcttctgcgtaaa
caacgcaccttcgataatggcagcatcccgcaccagattcacctgggcgaactgcatgcaattctccgccgtcaggagg
atttctacccgtttctgaaagataaccgtgagaaaattgaaaaaattctcaccttccgtattccgtactacgtcggacc
tctcgcccgtgggaacagccgtttcgcgtggatgacccgtaagtcggaagaaacaatcacgccatggaacttcgaagaa
gtggtcgataaaggcgcctccgcacagtccttcattgagcgcatgaccaatttcgacaaaaaccttcccaacgaaaaag
ttctgccgaaacactcgctgctgtatgaatactttacggtgtacaatgaactgacgaaagttaaatacgtcaccgaagg
gatgcgtaagcccgcctttctttctggtgaacagaaaaaagcaattgtggatctgctgttcaaaacaaaccgtaaagtt
accgttaagcaactcaaagaagattattttaagaagatcgaatgtttcgattcggttgaaattagcggcgtcgaagatc
gtttcaacgcgtcgctgggcacctatcacgaccttctgaagatcatcaaagataaggacttcctggataatgaagagaa
cgaggatatccttgaagatatcgtgcttactttaaccttgttcgaagaccgcgaaatgatcgaagaacggctcaaaaca
tatgcccatctgttcgatgacaaagttatgaaacagctgaaacgccgccgttacacgggttggggccgtttgtcgcgta
aactgattaatggcattcgcgacaaacagtcggggaaaaccattcttgattttctcaaatccgatggttttgccaatcg
caactttatgcagctcattcacgacgatagcctgaccttcaaggaagacatccagaaagcgcaagtgagcggccagggg
gacagtctgcacgagcacatcgcaaacctggccggaagcccggcgattaaaaaagggatcttacaaactgtcaaagttg
ttgatgaattagtaaaagtgatgggtcgccacaaaccggagaatatcgtaatcgaaatggctcgtgaaaaccaaaccac
tcagaaaggtcagaagaatagccgcgaacgtatgaaacgcattgaggaaggcatcaaagagttgggttcccagatttta
aaagagcatcctgtcgaaaatactcagctgcaaaatgaaaaattatacctgtactatctccagaatgggcgcgatatgt
atgtggaccaggaacttgacattaaccgtttaagtgactacgatgtggatcatattgttccgcaatcttttctcaaaga
tgattccattgataacaaagtgttaacccgttctgacaaaaatcgcgggaaatccgacaatgtaccgagcgaggaagtg
gtcaagaagatgaagaactattggcggcagctgctgaacgctaaactgatcacccagcgtaaatttgacaacctgacca
aagcagaacgtggtggactgagtgaattggacaaagcaggttttattaaacggcagctggtggaaacgcgccagattac
taagcacgtcgcgcaaattctggatagccgtatgaatacaaagtacgacgagaacgataagctcatccgcgaagtaaaa
gtcattaccctgaaatctaaactggtgagtgacttccgcaaagattttcagttttacaaggtccgggaaattaataact
accatcatgcccatgatgcgtacctgaatgcagtagtcggtacagcgttgattaaaaaatatccgaaactggagagtga
gttcgtttatggggactacaaagtttatgacgttcgtaaaatgattgcgaaaagtgagcaggaaattggcaaagcgacg
gcgaaatacttcttttatagtaacattatgaatttctttaagaccgaaatcacattagccaatggagaaatccgtaagc
gtccgctcatcgagacgaacggagaaaccggagaaattgtgtgggacaaaggtcgcgacttcgccacagtgcgtaaagt
tttatctatgccacaggttaacatcgtgaaaaaaacggaagtccaaacgggtggcttttctaaagaaagcattttgccc
aaacgcaacagtgataaattgattgcgcggaaaaaggattgggaccctaaaaaatatggcggctttgattctccaacag
tggcctattcggtgcttgtcgtggctaaagtggagaagggaaaatccaaaaagctgaaaagtgtaaaagaattgctggg
gatcacgatcatggaacgctcttcgttcgaaaaaaatccgatcgactttttagaagcaaaaggttataaagaggtgaag
aaagacctgatcattaaattaccgaaatatagcttgttcgagctggaaaatggtcgcaaacgtatgttggcctcagcgg
gagaattacaaaaaggcaacgaactcgcgcttccgagcaaatatgtcaatttcctttatctggcatcccattatgagaa
gctgaagggcagcccggaagataatgaacaaaaacagctgtttgtcgaacagcacaagcattatctcgatgaaatcatt
gaacaaattagcgaattttcaaaacgggtcatcctcgcagatgcaaaccttgacaaagttctgagcgcgtataataagc
atcgggataaaccaattcgcgaacaagcggaaaacatcattcatttatttacactgaccaacctgggcgcaccggcagc
gtttaaatatttcgatacgacaattgaccgtaaacgctacacctccacgaaagaagtgttggacgccaccctgattcac
cagagcatcacaggtctgtacgaaacgcgtattgatctgagtcagctgggaggcgactaatactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacggctagctcagtcctaggtatagtgct
agctactagagaaagaggagaaatactagatggcccaacaatcaccctattcagcagcgatggcagaacagcgtcacca
ggagtggttacgttttgtcgacctgcttaagaatgcctaccaaaacgatctccatttaccgttgttaaacctgatgctg
acgccagatgagcgcgaagcgttggggactcgcgtgcgtattgtcgaagagctgttgcgcggcgaaatgagccagcgtg
agttaaaaaatgaactcggcgcaggcatcgcgacgattacgcgtggatctaacagcctgaaagccgcgcccgtcgagct
gcgccagtggctggaagaggtgttgctgaaaagcgattgatactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttatatactagaggctgttgacaattaatcatcgaactagttaactagtacgcaagttcacgtac
tagaggttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggt
gc