BBa_K1774000
type: ComponentDefinition
persistent identity: BBa K1774000
display id: BBa_K1774000
version: 1
was generated by: iGEM to SBOL conversion
description: CRISPR-Cas9 contaiment device repressed by arabinose and tryptophan
created: 2015-09-17T11:00:00Z
modified: 2015-09-20T02:01:34Z
mutable provenance: Cas9 originated from S. pyogenes SF 370
owned by: myers
owned by: james
mutable description: This is a prototype containment device utilizing CRISPR-Cas9 system which aims to control accidental leakage of GMM once it escape from medium containing arabinose and tryptophan. The device consists of two modules, firstly a arabinose repressible cas9 expression module and a tryptophan repressible gRNA expression module. Under the presence of sufficient amount of arabinose and tryptophan the device remain silent, however when bacteria escape to environment without arabinose and tryptophan, Cas9 and a gRNA targeting DNA polymerase III alpha subunit will be expressed. Subsequent cleavage of the targeted sequence may hinder DNA replication and
discontinued: false
dominant: false
experience: experience/None
group u list: _2196_
m user id: 25503
owner id: 25503
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: a lot
star: false
creator: HKU iGEM 2015
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_K1774002
component: BBa_K1774003
component: BBa_J23106
component: BBa_B0015
component: BBa_K1774001
component: BBa_B0034
component: BBa_Q04510
component: BBa_K1333315
sequence annotation: BBa_K1333315
sequence annotation: BBa_Q04510
sequence annotation: BBa_B0034
sequence annotation: BBa_K1774001
sequence annotation: BBa_B0015
sequence annotation: BBa_J23106
sequence annotation: BBa_K1774003
sequence annotation: BBa_K1774002
sequence: https://synbiohub.org/public/igem/BBa_K1774000_sequence/1

BBa_K1774002
type: Component
persistent identity: component2474102
display id: component2474102
version: 1
top level: BBa_K1774000
definition: BBa_K1774002
access: public

BBa_K1774003
type: Component
persistent identity: component2474101
display id: component2474101
version: 1
top level: BBa_K1774000
definition: BBa_K1774003
access: public

BBa_J23106
type: Component
persistent identity: component2474086
display id: component2474086
version: 1
top level: BBa_K1774000
definition: BBa_J23106
access: public

BBa_B0015
type: Component
persistent identity: component2474085
display id: component2474085
version: 1
top level: BBa_K1774000
definition: BBa_B0015
access: public

BBa_K1774001
type: Component
persistent identity: component2474078
display id: component2474078
version: 1
top level: BBa_K1774000
definition: BBa_K1774001
access: public

BBa_B0034
type: Component
persistent identity: component2474077
display id: component2474077
version: 1
top level: BBa_K1774000
definition: BBa_B0034
access: public

BBa_Q04510
type: Component
persistent identity: component2474075
display id: component2474075
version: 1
top level: BBa_K1774000
definition: BBa_Q04510
access: public

BBa_K1333315
type: Component
persistent identity: component2474057
display id: component2474057
version: 1
top level: BBa_K1774000
definition: BBa_K1333315
access: public

BBa_K1333315
type: SequenceAnnotation
persistent identity: annotation2474057
display id: annotation2474057
version: 1
top level: BBa_K1774000
location: https://synbiohub.org/public/igem/BBa_K1774000/annotation2474057/range2474057/1
component: BBa_K1333315

https://synbiohub.org/public/igem/BBa_K1774000/annotation2474057/range2474057/1
type: Range
persistent identity: range2474057
display id: range2474057
version: 1
top level: BBa_K1774000
direction: direction/reverse
start: 1
end: 1252
orientation: inline

BBa_Q04510
type: SequenceAnnotation
persistent identity: annotation2474075
display id: annotation2474075
version: 1
top level: BBa_K1774000
location: https://synbiohub.org/public/igem/BBa_K1774000/annotation2474075/range2474075/1
component: BBa_Q04510

https://synbiohub.org/public/igem/BBa_K1774000/annotation2474075/range2474075/1
type: Range
persistent identity: range2474075
display id: range2474075
version: 1
top level: BBa_K1774000
direction: direction/reverse
start: 1261
end: 2247
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2474077
display id: annotation2474077
version: 1
top level: BBa_K1774000
location: https://synbiohub.org/public/igem/BBa_K1774000/annotation2474077/range2474077/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1774000/annotation2474077/range2474077/1
type: Range
persistent identity: range2474077
display id: range2474077
version: 1
top level: BBa_K1774000
direction: direction/reverse
start: 2256
end: 2267
orientation: inline

BBa_K1774001
type: SequenceAnnotation
persistent identity: annotation2474078
display id: annotation2474078
version: 1
top level: BBa_K1774000
location: https://synbiohub.org/public/igem/BBa_K1774000/annotation2474078/range2474078/1
component: BBa_K1774001

https://synbiohub.org/public/igem/BBa_K1774000/annotation2474078/range2474078/1
type: Range
persistent identity: range2474078
display id: range2474078
version: 1
top level: BBa_K1774000
direction: direction/reverse
start: 2274
end: 6380
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2474085
display id: annotation2474085
version: 1
top level: BBa_K1774000
location: https://synbiohub.org/public/igem/BBa_K1774000/annotation2474085/range2474085/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1774000/annotation2474085/range2474085/1
type: Range
persistent identity: range2474085
display id: range2474085
version: 1
top level: BBa_K1774000
direction: direction/reverse
start: 6389
end: 6517
orientation: inline

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2474086
display id: annotation2474086
version: 1
top level: BBa_K1774000
location: https://synbiohub.org/public/igem/BBa_K1774000/annotation2474086/range2474086/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_K1774000/annotation2474086/range2474086/1
type: Range
persistent identity: range2474086
display id: range2474086
version: 1
top level: BBa_K1774000
direction: direction/reverse
start: 6526
end: 6560
orientation: inline

BBa_K1774003
type: SequenceAnnotation
persistent identity: annotation2474101
display id: annotation2474101
version: 1
top level: BBa_K1774000
location: https://synbiohub.org/public/igem/BBa_K1774000/annotation2474101/range2474101/1
component: BBa_K1774003

https://synbiohub.org/public/igem/BBa_K1774000/annotation2474101/range2474101/1
type: Range
persistent identity: range2474101
display id: range2474101
version: 1
top level: BBa_K1774000
direction: direction/reverse
start: 6569
end: 7107
orientation: inline

BBa_K1774002
type: SequenceAnnotation
persistent identity: annotation2474102
display id: annotation2474102
version: 1
top level: BBa_K1774000
location: https://synbiohub.org/public/igem/BBa_K1774000/annotation2474102/range2474102/1
component: BBa_K1774002

https://synbiohub.org/public/igem/BBa_K1774000/annotation2474102/range2474102/1
type: Range
persistent identity: range2474102
display id: range2474102
version: 1
top level: BBa_K1774000
direction: direction/reverse
start: 7116
end: 7191
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_Q04510
type: ComponentDefinition
persistent identity: BBa Q04510
display id: BBa_Q04510
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0034.C0051.B0015.R0051)
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:14Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Lambda cI QPI w/ strong RBS
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_R0051
component: BBa_B0012
component: BBa_B0010
component: BBa_C0051
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0051
sequence: https://synbiohub.org/public/igem/BBa_Q04510_sequence/1

BBa_R0051
type: Component
persistent identity: component952926
display id: component952926
version: 1
top level: BBa_Q04510
definition: cI lam
access: public

BBa_B0012
type: Component
persistent identity: component952911
display id: component952911
version: 1
top level: BBa_Q04510
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component952901
display id: component952901
version: 1
top level: BBa_Q04510
definition: BBa_B0010
access: public

BBa_C0051
type: Component
persistent identity: component952895
display id: component952895
version: 1
top level: BBa_Q04510
definition: cI lam
access: public

BBa_B0034
type: Component
persistent identity: component952885
display id: component952885
version: 1
top level: BBa_Q04510
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation952885
display id: annotation952885
version: 1
top level: BBa_Q04510
location: https://synbiohub.org/public/igem/BBa_Q04510/annotation952885/range952885/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_Q04510/annotation952885/range952885/1
type: Range
persistent identity: range952885
display id: range952885
version: 1
top level: BBa_Q04510
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation952895
display id: annotation952895
version: 1
top level: BBa_Q04510
location: https://synbiohub.org/public/igem/BBa_Q04510/annotation952895/range952895/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_Q04510/annotation952895/range952895/1
type: Range
persistent identity: range952895
display id: range952895
version: 1
top level: BBa_Q04510
direction: direction/forward
start: 19
end: 768
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation952901
display id: annotation952901
version: 1
top level: BBa_Q04510
location: https://synbiohub.org/public/igem/BBa_Q04510/annotation952901/range952901/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q04510/annotation952901/range952901/1
type: Range
persistent identity: range952901
display id: range952901
version: 1
top level: BBa_Q04510
direction: direction/forward
start: 802
end: 881
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation952911
display id: annotation952911
version: 1
top level: BBa_Q04510
location: https://synbiohub.org/public/igem/BBa_Q04510/annotation952911/range952911/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q04510/annotation952911/range952911/1
type: Range
persistent identity: range952911
display id: range952911
version: 1
top level: BBa_Q04510
direction: direction/forward
start: 890
end: 930
orientation: inline

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation952926
display id: annotation952926
version: 1
top level: BBa_Q04510
location: https://synbiohub.org/public/igem/BBa_Q04510/annotation952926/range952926/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_Q04510/annotation952926/range952926/1
type: Range
persistent identity: range952926
display id: range952926
version: 1
top level: BBa_Q04510
direction: direction/forward
start: 939
end: 987
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23106
type: ComponentDefinition
persistent identity: BBa J23106
display id: BBa_J23106
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23106_sequence/1

BBa_K1774001
type: ComponentDefinition
persistent identity: BBa K1774001
display id: BBa_K1774001
version: 1
was generated by: iGEM to SBOL conversion
description: Cas9 (optimized for expression in E. coli)
created: 2015-09-17T11:00:00Z
modified: 2015-09-20T02:01:59Z
mutable provenance: S. pyogenes SF 370
owned by: james
owned by: myers
mutable description: This part code for cas9 originated from S. pyogenes SF 370 with codon optimized for expression in E. coli.
discontinued: false
dominant: false
experience: experience/None
group u list: _2196_
m user id: 25503
owner id: 25503
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Presence of illegal RE sites
star: false
creator: HKU iGEM 2015
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1774001_sequence/1

BBa_K1774002
type: ComponentDefinition
persistent identity: BBa K1774002
display id: BBa_K1774002
version: 1
was generated by: iGEM to SBOL conversion
description: gRNA for cas9 targeting DNA polyerase III alpha subunit
created: 2015-09-17T11:00:00Z
modified: 2015-09-20T02:02:24Z
mutable provenance: Generated from sequence of DNA polyerase III alpha subunit in E. coli BL21 DE3
owned by: james
owned by: myers
mutable description: gRNA targeting DNA polyerase III alpha subunit in E. coli BL21 DE3
discontinued: false
dominant: false
experience: experience/None
group u list: _2196_
m user id: 25503
owner id: 25503
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: No
star: false
creator: HKU iGEM 2015
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence: https://synbiohub.org/public/igem/BBa_K1774002_sequence/1

BBa_K1774003
type: ComponentDefinition
persistent identity: BBa K1774003
display id: BBa_K1774003
version: 1
was generated by: iGEM to SBOL conversion
description: trpR-pTrp inverter (RBS-trpR-TT-pTrp)
created: 2015-09-17T11:00:00Z
modified: 2015-09-20T02:02:42Z
mutable provenance: Modified from Trp operon
owned by: james
owned by: myers
mutable description: Addition of Trp repress anything downstream of the inverter. Notice that it contain illegal SpeI sites, however it it essential for the functioning of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _2196_
m user id: 25503
owner id: 25503
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Two illegal sites
star: false
creator: HKU iGEM 2015
type: DnaRegion
role: partType/Inverter
role: SO:0000804
component: BBa_K588001
component: BBa_B0015
component: BBa_K588000
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K588000
sequence annotation: BBa_B0015
sequence annotation: BBa_K588001
sequence: https://synbiohub.org/public/igem/BBa_K1774003_sequence/1

BBa_K588001
type: Component
persistent identity: component2474006
display id: component2474006
version: 1
top level: BBa_K1774003
definition: BBa_K588001
access: public

BBa_B0015
type: Component
persistent identity: component2474003
display id: component2474003
version: 1
top level: BBa_K1774003
definition: BBa_B0015
access: public

BBa_K588000
type: Component
persistent identity: component2473996
display id: component2473996
version: 1
top level: BBa_K1774003
definition: BBa_K588000
access: public

BBa_B0034
type: Component
persistent identity: component2473994
display id: component2473994
version: 1
top level: BBa_K1774003
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2473994
display id: annotation2473994
version: 1
top level: BBa_K1774003
location: https://synbiohub.org/public/igem/BBa_K1774003/annotation2473994/range2473994/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1774003/annotation2473994/range2473994/1
type: Range
persistent identity: range2473994
display id: range2473994
version: 1
top level: BBa_K1774003
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K588000
type: SequenceAnnotation
persistent identity: annotation2473996
display id: annotation2473996
version: 1
top level: BBa_K1774003
location: https://synbiohub.org/public/igem/BBa_K1774003/annotation2473996/range2473996/1
component: BBa_K588000

https://synbiohub.org/public/igem/BBa_K1774003/annotation2473996/range2473996/1
type: Range
persistent identity: range2473996
display id: range2473996
version: 1
top level: BBa_K1774003
direction: direction/reverse
start: 19
end: 345
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2474003
display id: annotation2474003
version: 1
top level: BBa_K1774003
location: https://synbiohub.org/public/igem/BBa_K1774003/annotation2474003/range2474003/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1774003/annotation2474003/range2474003/1
type: Range
persistent identity: range2474003
display id: range2474003
version: 1
top level: BBa_K1774003
direction: direction/reverse
start: 354
end: 482
orientation: inline

BBa_K588001
type: SequenceAnnotation
persistent identity: annotation2474006
display id: annotation2474006
version: 1
top level: BBa_K1774003
location: https://synbiohub.org/public/igem/BBa_K1774003/annotation2474006/range2474006/1
component: BBa_K588001

https://synbiohub.org/public/igem/BBa_K1774003/annotation2474006/range2474006/1
type: Range
persistent identity: range2474006
display id: range2474006
version: 1
top level: BBa_K1774003
direction: direction/reverse
start: 491
end: 539
orientation: inline

BBa_K1333315
type: ComponentDefinition
persistent identity: BBa K1333315
display id: BBa_K1333315
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD with araC
created: 2014-09-23T11:00:00Z
modified: 2015-05-08T01:09:58Z
mutable provenance: The two parts are all from previous parts.
owned by: james
owned by: myers
mutable description: A araBAD promoter with araC transcripted from the negative direction.
discontinued: false
dominant: false
group u list: _1708_
m user id: 0
owner id: 15896
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: We generate this regulatory part to regulate our reporter gene expression in our project.
star: false
creator: Wei Tang
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_I13453
component: BBa_I13458
sequence annotation: BBa_I13458
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K1333315_sequence/1

BBa_I13453
type: Component
persistent identity: component2386113
display id: component2386113
version: 1
top level: BBa_K1333315
definition: BBa_I13453
access: public

BBa_I13458
type: Component
persistent identity: component2386112
display id: component2386112
version: 1
top level: BBa_K1333315
definition: BBa_I13458
access: public

BBa_I13458
type: SequenceAnnotation
persistent identity: annotation2386112
display id: annotation2386112
version: 1
top level: BBa_K1333315
location: https://synbiohub.org/public/igem/BBa_K1333315/annotation2386112/range2386112/1
component: BBa_I13458

https://synbiohub.org/public/igem/BBa_K1333315/annotation2386112/range2386112/1
type: Range
persistent identity: range2386112
display id: range2386112
version: 1
top level: BBa_K1333315
direction: direction/reverse
start: 1
end: 1114
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2386113
display id: annotation2386113
version: 1
top level: BBa_K1333315
location: https://synbiohub.org/public/igem/BBa_K1333315/annotation2386113/range2386113/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K1333315/annotation2386113/range2386113/1
type: Range
persistent identity: range2386113
display id: range2386113
version: 1
top level: BBa_K1333315
direction: direction/reverse
start: 1123
end: 1252
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_I13458
type: ComponentDefinition
persistent identity: BBa I13458
display id: BBa_I13458
version: 1
was generated by: iGEM to SBOL conversion
description: promoter C and arabinose C operon
created: 2005-08-16T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PC and AraC from the AraBAD operator. PC and AraC are on the complementary strand, reading right to left.
discontinued: false
dominant: false
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Josh Michener
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence: https://synbiohub.org/public/igem/BBa_I13458_sequence/1

BBa_K588000
type: ComponentDefinition
persistent identity: BBa K588000
display id: BBa_K588000
version: 1
was generated by: iGEM to SBOL conversion
description: trpR
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:47Z
mutable provenance: Cloned from plasmid pJPR2 described in Paluh and Yanofsky NAR 1986
owned by: james
owned by: myers
mutable description: E. coli tryptophan repressor
discontinued: false
dominant: true
experience: experience/None
group u list: _759_
m user id: 0
owner id: 9140
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Added RFC10 biobrick prefix and suffix
star: false
creator: Kevin Lu
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: trpR
sequence: https://synbiohub.org/public/igem/BBa_K588000_sequence/1

trpR
type: SequenceAnnotation
persistent identity: annotation2142452
display id: annotation2142452
version: 1
top level: BBa_K588000
location: https://synbiohub.org/public/igem/BBa_K588000/annotation2142452/range2142452/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K588000/annotation2142452/range2142452/1
type: Range
persistent identity: range2142452
display id: range2142452
version: 1
top level: BBa_K588000
direction: direction/forward
start: 1
end: 327
orientation: inline

BBa_K588001
type: ComponentDefinition
persistent identity: BBa K588001
display id: BBa_K588001
version: 1
was generated by: iGEM to SBOL conversion
description: ptrp
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:12:47Z
mutable provenance: Synthesized by IDT
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Tryptophan promoter and operator
discontinued: false
dominant: false
experience: experience/None
group u list: _759_
m user id: 0
owner id: 9140
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The sequence contains SpeI sites at positions critical for function. We modified the SpeI site in the biobrick suffix to an NheI site as done by UNAM 2010
star: false
creator: Kevin Lu
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: ptrpL
sequence annotation: ptrpO
sequence: https://synbiohub.org/public/igem/BBa_K588001_sequence/1

ptrpL
type: SequenceAnnotation
persistent identity: annotation2142514
display id: annotation2142514
version: 1
top level: BBa_K588001
location: https://synbiohub.org/public/igem/BBa_K588001/annotation2142514/range2142514/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K588001/annotation2142514/range2142514/1
type: Range
persistent identity: range2142514
display id: range2142514
version: 1
top level: BBa_K588001
direction: direction/forward
start: 1
end: 49
orientation: inline

ptrpO
type: SequenceAnnotation
persistent identity: annotation2142550
display id: annotation2142550
version: 1
top level: BBa_K588001
location: https://synbiohub.org/public/igem/BBa_K588001/annotation2142550/range2142550/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K588001/annotation2142550/range2142550/1
type: Range
persistent identity: range2142550
display id: range2142550
version: 1
top level: BBa_K588001
direction: direction/forward
start: 20
end: 40
orientation: inline

https://synbiohub.org/public/igem/BBa_K1774000_sequence/1
type: Sequence
persistent identity: BBa K1774000 sequence
display id: BBa_K1774000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctattactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04510_sequence/1
type: Sequence
persistent identity: BBa Q04510 sequence
display id: BBa_Q04510_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23106_sequence/1
type: Sequence
persistent identity: BBa J23106 sequence
display id: BBa_J23106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1774001_sequence/1
type: Sequence
persistent identity: BBa K1774001 sequence
display id: BBa_K1774001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggacaaaaaatactcaatcggactggatattggtactaacagtgtgggctgggccgtaattaccgatgaatataaagtgccgagcaaaaaattcaaagtgctggggaacacggaccgtcattcgattaaaaaaaacctgatcggcgcactgctgttcgattccggcgaaaccgccgaagcgacgcgcctgaagcgcaccgcccggcgccgctatacccgtcgcaagaaccgtatctgttacctgcaagaaatcttcagcaacgaaatggcaaaagttgatgacagcttctttcatcgcttagaggaaagcttccttgtggaagaagacaaaaaacatgaacgccatcctatctttggcaatattgtagacgaagttgcctaccatgaaaagtatccgacgatttaccatttgcgtaaaaagctggtcgatagcactgataaagccgacctgcgtctgatctatctggcgctggcacacatgatcaaatttcgtggccattttttgattgaaggtgatcttaatccggacaattctgatgtcgacaaattgttcattcagctggtacagacctataaccaattgtttgaagaaaatccgattaacgcgagcggtgttgatgcaaaggccattctgtccgcccgtctgtcaaagagtcgtcgtcttgaaaacttgattgcgcaactgccgggcgaaaaaaaaaatgggcttttcggcaatctgatcgcattgtcgctgggtctgacccctaactttaagtcgaactttgatctcgcggaagacgccaagttacaactgagtaaagatacttatgatgatgatctggacaacctcctggctcagattggtgatcaatatgcggatctttttttagctgcgaagaacttgtctgatgcgattttactgtccgatattctgcgcgtgaacaccgagatcactaaagccccgttgagtgcctcaatgattaaacgctatgacgaacaccatcaggatctgacgctgctcaaagctctggttcgccagcagttaccagagaagtacaaggagatattcttcgatcagtctaagaacgggtacgcagggtacatcgatggtggtgcatcacaggaagagttctacaagttcattaaaccgattctggagaaaatggacggaacggaagaactgttggtgaaactgaaccgtgaggatcttctgcgtaaacaacgcaccttcgataatggcagcatcccgcaccagattcacctgggcgaactgcatgcaattctccgccgtcaggaggatttctacccgtttctgaaagataaccgtgagaaaattgaaaaaattctcaccttccgtattccgtactacgtcggacctctcgcccgtgggaacagccgtttcgcgtggatgacccgtaagtcggaagaaacaatcacgccatggaacttcgaagaagtggtcgataaaggcgcctccgcacagtccttcattgagcgcatgaccaatttcgacaaaaaccttcccaacgaaaaagttctgccgaaacactcgctgctgtatgaatactttacggtgtacaatgaactgacgaaagttaaatacgtcaccgaagggatgcgtaagcccgcctttctttctggtgaacagaaaaaagcaattgtggatctgctgttcaaaacaaaccgtaaagttaccgttaagcaactcaaagaagattattttaagaagatcgaatgtttcgattcggttgaaattagcggcgtcgaagatcgtttcaacgcgtcgctgggcacctatcacgaccttctgaagatcatcaaagataaggacttcctggataatgaagagaacgaggatatccttgaagatatcgtgcttactttaaccttgttcgaagaccgcgaaatgatcgaagaacggctcaaaacatatgcccatctgttcgatgacaaagttatgaaacagctgaaacgccgccgttacacgggttggggccgtttgtcgcgtaaactgat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1774002_sequence/1
type: Sequence
persistent identity: BBa K1774002 sequence
display id: BBa_K1774002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1774003_sequence/1
type: Sequence
persistent identity: BBa K1774003 sequence
display id: BBa_K1774003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcccaacaatcaccctattcagcagcgatggcagaacagcgtcaccaggagtggttacgttttgtcgacctgcttaagaatgcctaccaaaacgatctccatttaccgttgttaaacctgatgctgacgccagatgagcgcgaagcgttggggactcgcgtgcgtattgtcgaagagctgttgcgcggcgaaatgagccagcgtgagttaaaaaatgaactcggcgcaggcatcgcgacgattacgcgtggatctaacagcctgaaagccgcgcccgtcgagctgcgccagtggctggaagaggtgttgctgaaaagcgattgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggctgttgacaattaatcatcgaactagttaactagtacgcaagttcacg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1333315_sequence/1
type: Sequence
persistent identity: BBa K1333315 sequence
display id: BBa_K1333315_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctattactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13458_sequence/1
type: Sequence
persistent identity: BBa I13458 sequence
display id: BBa_I13458_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K588000_sequence/1
type: Sequence
persistent identity: BBa K588000 sequence
display id: BBa_K588000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcccaacaatcaccctattcagcagcgatggcagaacagcgtcaccaggagtggttacgttttgtcgacctgcttaagaatgcctaccaaaacgatctccatttaccgttgttaaacctgatgctgacgccagatgagcgcgaagcgttggggactcgcgtgcgtattgtcgaagagctgttgcgcggcgaaatgagccagcgtgagttaaaaaatgaactcggcgcaggcatcgcgacgattacgcgtggatctaacagcctgaaagccgcgcccgtcgagctgcgccagtggctggaagaggtgttgctgaaaagcgattga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K588001_sequence/1
type: Sequence
persistent identity: BBa K588001 sequence
display id: BBa_K588001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgttgacaattaatcatcgaactagttaactagtacgcaagttcacg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z