LOCUS BBa_K1774001 4107 bp DNA linear 25-Nov-2024 DEFINITION Cas9 (optimized for expression in E. coli) ACCESSION BBa_K1774001 VERSION BBa_K1774001.1 FEATURES Location/Qualifiers ORIGIN 1 atggacaaaa aatactcaat cggactggat attggtacta acagtgtggg ctgggccgta 61 attaccgatg aatataaagt gccgagcaaa aaattcaaag tgctggggaa cacggaccgt 121 cattcgatta aaaaaaacct gatcggcgca ctgctgttcg attccggcga aaccgccgaa 181 gcgacgcgcc tgaagcgcac cgcccggcgc cgctataccc gtcgcaagaa ccgtatctgt 241 tacctgcaag aaatcttcag caacgaaatg gcaaaagttg atgacagctt ctttcatcgc 301 ttagaggaaa gcttccttgt ggaagaagac aaaaaacatg aacgccatcc tatctttggc 361 aatattgtag acgaagttgc ctaccatgaa aagtatccga cgatttacca tttgcgtaaa 421 aagctggtcg atagcactga taaagccgac ctgcgtctga tctatctggc gctggcacac 481 atgatcaaat ttcgtggcca ttttttgatt gaaggtgatc ttaatccgga caattctgat 541 gtcgacaaat tgttcattca gctggtacag acctataacc aattgtttga agaaaatccg 601 attaacgcga gcggtgttga tgcaaaggcc attctgtccg cccgtctgtc aaagagtcgt 661 cgtcttgaaa acttgattgc gcaactgccg ggcgaaaaaa aaaatgggct tttcggcaat 721 ctgatcgcat tgtcgctggg tctgacccct aactttaagt cgaactttga tctcgcggaa 781 gacgccaagt tacaactgag taaagatact tatgatgatg atctggacaa cctcctggct 841 cagattggtg atcaatatgc ggatcttttt ttagctgcga agaacttgtc tgatgcgatt 901 ttactgtccg atattctgcg cgtgaacacc gagatcacta aagccccgtt gagtgcctca 961 atgattaaac gctatgacga acaccatcag gatctgacgc tgctcaaagc tctggttcgc 1021 cagcagttac cagagaagta caaggagata ttcttcgatc agtctaagaa cgggtacgca 1081 gggtacatcg atggtggtgc atcacaggaa gagttctaca agttcattaa accgattctg 1141 gagaaaatgg acggaacgga agaactgttg gtgaaactga accgtgagga tcttctgcgt 1201 aaacaacgca ccttcgataa tggcagcatc ccgcaccaga ttcacctggg cgaactgcat 1261 gcaattctcc gccgtcagga ggatttctac ccgtttctga aagataaccg tgagaaaatt 1321 gaaaaaattc tcaccttccg tattccgtac tacgtcggac ctctcgcccg tgggaacagc 1381 cgtttcgcgt ggatgacccg taagtcggaa gaaacaatca cgccatggaa cttcgaagaa 1441 gtggtcgata aaggcgcctc cgcacagtcc ttcattgagc gcatgaccaa tttcgacaaa 1501 aaccttccca acgaaaaagt tctgccgaaa cactcgctgc tgtatgaata ctttacggtg 1561 tacaatgaac tgacgaaagt taaatacgtc accgaaggga tgcgtaagcc cgcctttctt 1621 tctggtgaac agaaaaaagc aattgtggat ctgctgttca aaacaaaccg taaagttacc 1681 gttaagcaac tcaaagaaga ttattttaag aagatcgaat gtttcgattc ggttgaaatt 1741 agcggcgtcg aagatcgttt caacgcgtcg ctgggcacct atcacgacct tctgaagatc 1801 atcaaagata aggacttcct ggataatgaa gagaacgagg atatccttga agatatcgtg 1861 cttactttaa ccttgttcga agaccgcgaa atgatcgaag aacggctcaa aacatatgcc 1921 catctgttcg atgacaaagt tatgaaacag ctgaaacgcc gccgttacac gggttggggc 1981 cgtttgtcgc gtaaactgat taatggcatt cgcgacaaac agtcggggaa aaccattctt 2041 gattttctca aatccgatgg ttttgccaat cgcaacttta tgcagctcat tcacgacgat 2101 agcctgacct tcaaggaaga catccagaaa gcgcaagtga gcggccaggg ggacagtctg 2161 cacgagcaca tcgcaaacct ggccggaagc ccggcgatta aaaaagggat cttacaaact 2221 gtcaaagttg ttgatgaatt agtaaaagtg atgggtcgcc acaaaccgga gaatatcgta 2281 atcgaaatgg ctcgtgaaaa ccaaaccact cagaaaggtc agaagaatag ccgcgaacgt 2341 atgaaacgca ttgaggaagg catcaaagag ttgggttccc agattttaaa agagcatcct 2401 gtcgaaaata ctcagctgca aaatgaaaaa ttatacctgt actatctcca gaatgggcgc 2461 gatatgtatg tggaccagga acttgacatt aaccgtttaa gtgactacga tgtggatcat 2521 attgttccgc aatcttttct caaagatgat tccattgata acaaagtgtt aacccgttct 2581 gacaaaaatc gcgggaaatc cgacaatgta ccgagcgagg aagtggtcaa gaagatgaag 2641 aactattggc ggcagctgct gaacgctaaa ctgatcaccc agcgtaaatt tgacaacctg 2701 accaaagcag aacgtggtgg actgagtgaa ttggacaaag caggttttat taaacggcag 2761 ctggtggaaa cgcgccagat tactaagcac gtcgcgcaaa ttctggatag ccgtatgaat 2821 acaaagtacg acgagaacga taagctcatc cgcgaagtaa aagtcattac cctgaaatct 2881 aaactggtga gtgacttccg caaagatttt cagttttaca aggtccggga aattaataac 2941 taccatcatg cccatgatgc gtacctgaat gcagtagtcg gtacagcgtt gattaaaaaa 3001 tatccgaaac tggagagtga gttcgtttat ggggactaca aagtttatga cgttcgtaaa 3061 atgattgcga aaagtgagca ggaaattggc aaagcgacgg cgaaatactt cttttatagt 3121 aacattatga atttctttaa gaccgaaatc acattagcca atggagaaat ccgtaagcgt 3181 ccgctcatcg agacgaacgg agaaaccgga gaaattgtgt gggacaaagg tcgcgacttc 3241 gccacagtgc gtaaagtttt atctatgcca caggttaaca tcgtgaaaaa aacggaagtc 3301 caaacgggtg gcttttctaa agaaagcatt ttgcccaaac gcaacagtga taaattgatt 3361 gcgcggaaaa aggattggga ccctaaaaaa tatggcggct ttgattctcc aacagtggcc 3421 tattcggtgc ttgtcgtggc taaagtggag aagggaaaat ccaaaaagct gaaaagtgta 3481 aaagaattgc tggggatcac gatcatggaa cgctcttcgt tcgaaaaaaa tccgatcgac 3541 tttttagaag caaaaggtta taaagaggtg aagaaagacc tgatcattaa attaccgaaa 3601 tatagcttgt tcgagctgga aaatggtcgc aaacgtatgt tggcctcagc gggagaatta 3661 caaaaaggca acgaactcgc gcttccgagc aaatatgtca atttccttta tctggcatcc 3721 cattatgaga agctgaaggg cagcccggaa gataatgaac aaaaacagct gtttgtcgaa 3781 cagcacaagc attatctcga tgaaatcatt gaacaaatta gcgaattttc aaaacgggtc 3841 atcctcgcag atgcaaacct tgacaaagtt ctgagcgcgt ataataagca tcgggataaa 3901 ccaattcgcg aacaagcgga aaacatcatt catttattta cactgaccaa cctgggcgca 3961 ccggcagcgt ttaaatattt cgatacgaca attgaccgta aacgctacac ctccacgaaa 4021 gaagtgttgg acgccaccct gattcaccag agcatcacag gtctgtacga aacgcgtatt 4081 gatctgagtc agctgggagg cgactaa //