BBa_K1774001 1 BBa_K1774001 Cas9 (optimized for expression in E. coli) 2015-09-17T11:00:00Z 2015-09-20T02:01:59Z S. pyogenes SF 370 This part code for cas9 originated from S. pyogenes SF 370 with codon optimized for expression in E. coli. false false _2196_ 25503 25503 9 false Presence of illegal RE sites false HKU iGEM 2015 BBa_K1774001_sequence 1 atggacaaaaaatactcaatcggactggatattggtactaacagtgtgggctgggccgtaattaccgatgaatataaagtgccgagcaaaaaattcaaagtgctggggaacacggaccgtcattcgattaaaaaaaacctgatcggcgcactgctgttcgattccggcgaaaccgccgaagcgacgcgcctgaagcgcaccgcccggcgccgctatacccgtcgcaagaaccgtatctgttacctgcaagaaatcttcagcaacgaaatggcaaaagttgatgacagcttctttcatcgcttagaggaaagcttccttgtggaagaagacaaaaaacatgaacgccatcctatctttggcaatattgtagacgaagttgcctaccatgaaaagtatccgacgatttaccatttgcgtaaaaagctggtcgatagcactgataaagccgacctgcgtctgatctatctggcgctggcacacatgatcaaatttcgtggccattttttgattgaaggtgatcttaatccggacaattctgatgtcgacaaattgttcattcagctggtacagacctataaccaattgtttgaagaaaatccgattaacgcgagcggtgttgatgcaaaggccattctgtccgcccgtctgtcaaagagtcgtcgtcttgaaaacttgattgcgcaactgccgggcgaaaaaaaaaatgggcttttcggcaatctgatcgcattgtcgctgggtctgacccctaactttaagtcgaactttgatctcgcggaagacgccaagttacaactgagtaaagatacttatgatgatgatctggacaacctcctggctcagattggtgatcaatatgcggatctttttttagctgcgaagaacttgtctgatgcgattttactgtccgatattctgcgcgtgaacaccgagatcactaaagccccgttgagtgcctcaatgattaaacgctatgacgaacaccatcaggatctgacgctgctcaaagctctggttcgccagcagttaccagagaagtacaaggagatattcttcgatcagtctaagaacgggtacgcagggtacatcgatggtggtgcatcacaggaagagttctacaagttcattaaaccgattctggagaaaatggacggaacggaagaactgttggtgaaactgaaccgtgaggatcttctgcgtaaacaacgcaccttcgataatggcagcatcccgcaccagattcacctgggcgaactgcatgcaattctccgccgtcaggaggatttctacccgtttctgaaagataaccgtgagaaaattgaaaaaattctcaccttccgtattccgtactacgtcggacctctcgcccgtgggaacagccgtttcgcgtggatgacccgtaagtcggaagaaacaatcacgccatggaacttcgaagaagtggtcgataaaggcgcctccgcacagtccttcattgagcgcatgaccaatttcgacaaaaaccttcccaacgaaaaagttctgccgaaacactcgctgctgtatgaatactttacggtgtacaatgaactgacgaaagttaaatacgtcaccgaagggatgcgtaagcccgcctttctttctggtgaacagaaaaaagcaattgtggatctgctgttcaaaacaaaccgtaaagttaccgttaagcaactcaaagaagattattttaagaagatcgaatgtttcgattcggttgaaattagcggcgtcgaagatcgtttcaacgcgtcgctgggcacctatcacgaccttctgaagatcatcaaagataaggacttcctggataatgaagagaacgaggatatccttgaagatatcgtgcttactttaaccttgttcgaagaccgcgaaatgatcgaagaacggctcaaaacatatgcccatctgttcgatgacaaagttatgaaacagctgaaacgccgccgttacacgggttggggccgtttgtcgcgtaaactgattaatggcattcgcgacaaacagtcggggaaaaccattcttgattttctcaaatccgatggttttgccaatcgcaactttatgcagctcattcacgacgatagcctgaccttcaaggaagacatccagaaagcgcaagtgagcggccagggggacagtctgcacgagcacatcgcaaacctggccggaagcccggcgattaaaaaagggatcttacaaactgtcaaagttgttgatgaattagtaaaagtgatgggtcgccacaaaccggagaatatcgtaatcgaaatggctcgtgaaaaccaaaccactcagaaaggtcagaagaatagccgcgaacgtatgaaacgcattgaggaaggcatcaaagagttgggttcccagattttaaaagagcatcctgtcgaaaatactcagctgcaaaatgaaaaattatacctgtactatctccagaatgggcgcgatatgtatgtggaccaggaacttgacattaaccgtttaagtgactacgatgtggatcatattgttccgcaatcttttctcaaagatgattccattgataacaaagtgttaacccgttctgacaaaaatcgcgggaaatccgacaatgtaccgagcgaggaagtggtcaagaagatgaagaactattggcggcagctgctgaacgctaaactgatcacccagcgtaaatttgacaacctgaccaaagcagaacgtggtggactgagtgaattggacaaagcaggttttattaaacggcagctggtggaaacgcgccagattactaagcacgtcgcgcaaattctggatagccgtatgaatacaaagtacgacgagaacgataagctcatccgcgaagtaaaagtcattaccctgaaatctaaactggtgagtgacttccgcaaagattttcagttttacaaggtccgggaaattaataactaccatcatgcccatgatgcgtacctgaatgcagtagtcggtacagcgttgattaaaaaatatccgaaactggagagtgagttcgtttatggggactacaaagtttatgacgttcgtaaaatgattgcgaaaagtgagcaggaaattggcaaagcgacggcgaaatacttcttttatagtaacattatgaatttctttaagaccgaaatcacattagccaatggagaaatccgtaagcgtccgctcatcgagacgaacggagaaaccggagaaattgtgtgggacaaaggtcgcgacttcgccacagtgcgtaaagttttatctatgccacaggttaacatcgtgaaaaaaacggaagtccaaacgggtggcttttctaaagaaagcattttgcccaaacgcaacagtgataaattgattgcgcggaaaaaggattgggaccctaaaaaatatggcggctttgattctccaacagtggcctattcggtgcttgtcgtggctaaagtggagaagggaaaatccaaaaagctgaaaagtgtaaaagaattgctggggatcacgatcatggaacgctcttcgttcgaaaaaaatccgatcgactttttagaagcaaaaggttataaagaggtgaagaaagacctgatcattaaattaccgaaatatagcttgttcgagctggaaaatggtcgcaaacgtatgttggcctcagcgggagaattacaaaaaggcaacgaactcgcgcttccgagcaaatatgtcaatttcctttatctggcatcccattatgagaagctgaagggcagcccggaagataatgaacaaaaacagctgtttgtcgaacagcacaagcattatctcgatgaaatcattgaacaaattagcgaattttcaaaacgggtcatcctcgcagatgcaaaccttgacaaagttctgagcgcgtataataagcatcgggataaaccaattcgcgaacaagcggaaaacatcattcatttatttacactgaccaacctgggcgcaccggcagcgtttaaatatttcgatacgacaattgaccgtaaacgctacacctccacgaaagaagtgttggacgccaccctgattcaccagagcatcacaggtctgtacgaaacgcgtattgatctgagtcagctgggaggcgactaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z