BBa_K1777019
1
BBa_K1777019
flexible linker
2015-09-17T11:00:00Z
2015-09-20T02:06:57Z
BBa_K1486003
This a flexible linker combined by two former linkers, which can be easily connect to the end of protein with overlap pcr.
false
false
_2199_
27798
25645
9
false
when we chose a linker for our fusion protein,we would have wanted to use -gggsgggs- linker. But we found that such a tandem repeating linker is hard to work well with overlap PCR because the OE region should be unique. Therefore, we improved the original part by change the first GGGS to a carefully selected natural linker from linker database to avoid repeating sequence. Besides, we used the selected natural linker to adjust its length to about 50A.
false
Xuhang Li
annotation2476148
1
natural linker
range2476148
1
1
21
annotation2476149
1
GS linker
range2476149
1
22
36
BBa_K1777019_sequence
1
cctcctccataccagcctctcggcggcggcggcagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z