BBa_K1778003 1 BBa_K1778003 RtTA-flag is a regulatory protein that can regulate the binding of tetracycline analogues. 2015-09-10T11:00:00Z 2015-09-11T09:32:28Z rtTA is from the commercial plasmid pTet-on,flag tags is commonly used detective tags. RtTA-flag is a regulatory protein that can regulate the binding of tetracycline analogues.On the N side, a flag tag is brought.The purpose of the design is to verify that the rtTA regulatory proteins are able to be stably expressed in yeast cells, and the role of the project is to be verified. false false _2200_ 25918 25918 9 false When using the part in Pichia pastoris, we need to consider whether the protein is stable in yeast cells and whether the formation of its conformation is effective to identify specific promoter, which requires the support of experiments and the paper. false Lehua Jia BBa_K1778003_sequence 1 atgtctagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagcttggtgtagagcagcctacactgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttaaaaggggaaagctggcaagattttttacgcaataacgctaaaagttttagatgtgctttactaagtcatcgcaatggagcaaaagtacattcagatacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaacgcgttatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgcgtacagccgcgcgcgtacgaaaaacaattacgggtctaccatcgagggcctgctcgatctcccggacgacgacgcccccgaagaggcggggctggcggctccgcgcctgtcctttctccccgcgggacacacgcgcagactgtcgacggcccccccgaccgatgtcagcctgggggacgagctccacttagacggcgaggacgtggcgatggcgcatgccgacgcgctagacgatttcgatctggacatgttgggggacggggattccccgggtccgggatttaccccccacgactccgccccctacggcgctctggatatggccgacttcgagtttgagcagatgtttaccgatgcccttggaattgacgagtacggtgggtaggattacaaggatgatgacgataag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z