BBa_K1778004 1 BBa_K1778004 rtTA is a regulatory protein which can combined with tetracycline analogues. 2015-09-10T11:00:00Z 2015-09-11T10:09:10Z rtTA is from the plasmid pTet-on, It was merged by reverse TetR and the transcriptional activation area of VP16. rtTA is a regulatory protein which can combined with tetracycline analogues. Like the protein in Biological signaling pathways. When it combine with tetracycline analogues, it can change its conformation to activate the subsequent signal path. Under the action of constitutive promoter, the regulatory protein expresses stably. When it combine with tetracycline analogues, rtTA change its conformation to activate the promoter, then the report gene expresses. false false _2200_ 25918 25918 9 false When we use the part in pichia pastoris, we need to think that if the protein can exist stably in yeast cell and activate the promoter effectively. It need to be proved by experiment and references. false Lehua Jia BBa_K1778004_sequence 1 atgtctagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagcttggtgtagagcagcctacactgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttaaaaggggaaagctggcaagattttttacgcaataacgctaaaagttttagatgtgctttactaagtcatcgcaatggagcaaaagtacattcagatacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaacgcgttatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgcgtacagccgcgcgcgtacgaaaaacaattacgggtctaccatcgagggcctgctcgatctcccggacgacgacgcccccgaagaggcggggctggcggctccgcgcctgtcctttctccccgcgggacacacgcgcagactgtcgacggcccccccgaccgatgtcagcctgggggacgagctccacttagacggcgaggacgtggcgatggcgcatgccgacgcgctagacgatttcgatctggacatgttgggggacggggattccccgggtccgggatttaccccccacgactccgccccctacggcgctctggatatggccgacttcgagtttgagcagatgtttaccgatgcccttggaattgacgagtacggtgggtag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z