BBa_K1779200 1 BBa_K1779200 Integration of three genes, including mamW, RFP and laccase 2015-09-02T11:00:00Z 2015-09-07T02:04:21Z mamW gene is from MTB's genomic sequence. RFP gene is obtained from the iGEM official bio bricks, which numbered BBa_E1010. Laccase gene is obtained from the iGEM official bio bricks, which numbered BBa_K863005. ntegration of three genes, including mamW, RFP and laccase.Where mamW gene is isolated from MTB bacilli obtained can be used as an anchor protein.RFP gene is extracted from iGEM official bio bricks, in this experiment, as a reporter gene expression can display fusion gene.Laccase gene is also obtained from the iGEM official bio bricks and paint as an oxidase enzyme, in this experiment, as the cathodic reaction main substance involved in the biofuel cell. false false _2204_ 25495 25495 9 It's complicated false We designed primers with linker will mamW, RFP and laccase three genes linked by PCR of fusion.The mamW and RFP genes'stop codons have been removed. false Xingyang Qiu annotation2445395 1 G4S3 linker range2445395 1 1126 1170 annotation2445391 1 mamaW range2445391 1 1 414 annotation2445394 1 RFP range2445394 1 451 1125 annotation2445392 1 A to G range2445392 1 264 264 annotation2445396 1 laccase range2445396 1 1171 2721 annotation2445393 1 G3S2 linker range2445393 1 415 450 BBa_K1779200_sequence 1 atgctaactgcgatcattgccggaatcttgctgtttctgttcggggttctcatcaagggcgtgttcgcgcacgccatggtcattctcgtcgcttggctggccaagaacggcctgctgctggcattcctgagaacccgcttcggtcagcgggtggttcgaaacgtgcgcttcaagacctatagccaagtcggtcagggaagcgggcggcggcgcgcctatcatgtcttcaagcgcatggcgggggccgagcgcggaatcgtccggattctgatccgggtgaaggatgggatcgccttgattctttgggggccgaaagcagtgagaaaaccgtcctctgctccgcgttccggcaccacgccccgtatcgccgcctctccgacggcttcgccgattcggaagcaatcgcctggactcgcaggtagcggcagcggtagcggtagcggcagcgcgatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctggtggtggtggttctggcggcggcggctccggtggtggtggttctatgcaacgtcgtgatttcttaaaatattccgtcgcgctgggtgtggcttcggctttgccgctgtggaaccgcgcagtatttgcggcagaacgcccaacgttaccgatccctgatttgctcacgaccgatgcccgtaatcgcattcagttaactattggcgcaggccagtccacctttggcgggaaaactgcaactacctggggctataacggcaatctgctgggaccggcggtgaaattacagcgcggcaaagcggtaacggttgatatctacaaccaactgacggaagagacaacgttgcactggcacgggctggaagtaccgggtgaagtcgacggcggcccgcagggaattattccgccaggtggcaagcgctcggtgacgttgaacgttgatcaacctgccgctacctgctggttccatccgcatcagcacggcaaaaccgggcgacaggtggcgatggggctggctgggctggtggtgattgaagatgacgagatcctgaaattaatgctgccaaaacagtggggtatcgatgatgttccggtgatcgttcaggataagaaatttagcgccgacgggcagattgattatcaactggatgtgatgaccgccgccgtgggctggtttggcgatacgttgctgaccaacggtgcaatctacccgcaacacgctgccccgcgtggttggctgcgcctgcgtttgctcaatggctgtaatgcccgttcgctcaatttcgccaccagcgacaatcgcccgctgtatgtgattgccagcgacggtggtctgctacctgaaccagtgaaggtgagcgaactgccggtgctgatgggcgagcgttttgaagtgctggtggaggttaacgataacaaaccctttgacctggtgacgctgccggtcagccagatggggatggcgattgcgccgtttgataagcctcatccggtaatgcggattcagccgattgctattagtgcctccggtgctttgccagacacattaagtagcctgcctgcgttaccttcgctggaagggctgacggtacgcaagctgcaactctctatggacccgatgctcgatatgatggggatgcagatgctaatggagaaatatggcgatcaggcgatggccgggatggatcacagccagatgatgggccatatggggcacggcaatatgaatcatatgaaccacggcgggaagttcgatttccaccatgccaacaaaatcaacggtcaggcgtttgatatgaacaagccgatgtttgcggcggcgaaagggcaatacgaacgttgggttatctctggcgtgggcgacatgatgctgcatccgttccatatccacggcacgcagttccgtatcttgtcagaaaatggcaaaccgccagcggctcatcgcgcgggctggaaagataccgttaaggtagaaggtaatgtcagcgaagtgctggtgaagtttaatcacgatgcaccgaaagaacatgcttatatggcgcactgccatctgctggagcatgaagatacggggatgatgttagggtttacggtataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z