LOCUS       BBa_K1779204            2311 bp    DNA     linear       22-Dec-2024
DEFINITION  Integration of twogenes, including RFP and laccase
ACCESSION   BBa_K1779204
VERSION     BBa_K1779204.1  
FEATURES             Location/Qualifiers
     CDS             1..675
                     /label=mrfp1
     misc_feature    682..706
                     /label=Help:Barcodes
     CDS             1..706
                     /label=BBa_E1010
     promoter        707..729
                     /label=T7 promoter
     misc_feature    740..742
                     /label=ATG
     misc_feature    2309..2311
                     /label=TAA
     misc_feature    2306..2308
                     /label=TAA
     misc_feature    2288..2305
                     /label=6x HIS 
     CDS             740..2287
                     /label=ecol
     RBS             731..736
                     /label=RBS
     CDS             707..2311
                     /label=BBa_K863005
ORIGIN
        1 atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt
       61 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt
      121 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc
      181 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg
      241 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa
      301 gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac
      361 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc
      421 atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa
      481 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc
      541 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac
      601 atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt
      661 cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctaat acgactcact
      721 atagggagag aggagaaaaa tgcaacgtcg tgatttctta aaatattccg tcgcgctggg
      781 tgtggcttcg gctttgccgc tgtggaaccg cgcagtattt gcggcagaac gcccaacgtt
      841 accgatccct gatttgctca cgaccgatgc ccgtaatcgc attcagttaa ctattggcgc
      901 aggccagtcc acctttggcg ggaaaactgc aactacctgg ggctataacg gcaatctgct
      961 gggaccggcg gtgaaattac agcgcggcaa agcggtaacg gttgatatct acaaccaact
     1021 gacggaagag acaacgttgc actggcacgg gctggaagta ccgggtgaag tcgacggcgg
     1081 cccgcaggga attattccgc caggtggcaa gcgctcggtg acgttgaacg ttgatcaacc
     1141 tgccgctacc tgctggttcc atccgcatca gcacggcaaa accgggcgac aggtggcgat
     1201 ggggctggct gggctggtgg tgattgaaga tgacgagatc ctgaaattaa tgctgccaaa
     1261 acagtggggt atcgatgatg ttccggtgat cgttcaggat aagaaattta gcgccgacgg
     1321 gcagattgat tatcaactgg atgtgatgac cgccgccgtg ggctggtttg gcgatacgtt
     1381 gctgaccaac ggtgcaatct acccgcaaca cgctgccccg cgtggttggc tgcgcctgcg
     1441 tttgctcaat ggctgtaatg cccgttcgct caatttcgcc accagcgaca atcgcccgct
     1501 gtatgtgatt gccagcgacg gtggtctgct acctgaacca gtgaaggtga gcgaactgcc
     1561 ggtgctgatg ggcgagcgtt ttgaagtgct ggtggaggtt aacgataaca aaccctttga
     1621 cctggtgacg ctgccggtca gccagatggg gatggcgatt gcgccgtttg ataagcctca
     1681 tccggtaatg cggattcagc cgattgctat tagtgcctcc ggtgctttgc cagacacatt
     1741 aagtagcctg cctgcgttac cttcgctgga agggctgacg gtacgcaagc tgcaactctc
     1801 tatggacccg atgctcgata tgatggggat gcagatgcta atggagaaat atggcgatca
     1861 ggcgatggcc gggatggatc acagccagat gatgggccat atggggcacg gcaatatgaa
     1921 tcatatgaac cacggcggga agttcgattt ccaccatgcc aacaaaatca acggtcaggc
     1981 gtttgatatg aacaagccga tgtttgcggc ggcgaaaggg caatacgaac gttgggttat
     2041 ctctggcgtg ggcgacatga tgctgcatcc gttccatatc cacggcacgc agttccgtat
     2101 cttgtcagaa aatggcaaac cgccagcggc tcatcgcgcg ggctggaaag ataccgttaa
     2161 ggtagaaggt aatgtcagcg aagtgctggt gaagtttaat cacgatgcac cgaaagaaca
     2221 tgcttatatg gcgcactgcc atctgctgga gcatgaagat acggggatga tgttagggtt
     2281 tacggtacat caccatcacc atcactaata a
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