LOCUS BBa_K1780001 6501 bp DNA linear 15-Nov-2024 DEFINITION variable link oscillator ACCESSION BBa_K1780001 VERSION BBa_K1780001.1 FEATURES Location/Qualifiers CDS 1..720 /label=GFP protein CDS 1..720 /label=BBa_E0040 promoter 1808..1938 /label=Pbad promoter 1771..1842 /label=PC misc_feature 729..1807 /label=AraC promoter 729..1938 /label=BBa_I0500 RBS 1947..1950 /label=BBa_K118012 CDS 1957..2868 /label=BBa_I742151 misc_feature 2877..2879 /label=SacI RBS 2880..2886 /label=rbs RBS 2877..2886 /label=BBa_J15001 CDS 2893..3822 /label=crtB coding sequence CDS 2893..3825 /label=BBa_K118002 misc_feature 3834..3836 /label=SacI RBS 3837..3843 /label=rbs RBS 3834..3843 /label=BBa_J15001 misc_feature 4327..4332 /label=PstI site removed misc_feature 4738..4743 /label=PstI site removed CDS 3850..5328 /label=crtI coding sequence CDS 3850..5331 /label=BBa_K118003 RBS 5340..5343 /label=BBa_K118012 CDS 5350..6498 /label=crtY coding sequence CDS 5350..6501 /label=BBa_K118008 misc_feature 729..6501 /label=BBa_K274220 ORIGIN 1 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt 61 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga 121 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt 181 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag 241 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc 301 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt 361 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa 421 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga 481 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac 541 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac 601 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt 661 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa 721 tactagagtt atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg 781 aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg 841 tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc 901 ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg 961 cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata 1021 tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta 1081 tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca 1141 agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt 1201 tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta 1261 ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa 1321 acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct 1381 ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc 1441 accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg 1501 ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca 1561 ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc 1621 ccgccattca gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc 1681 ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa 1741 caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga 1801 aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc 1861 ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatac 1921 ccgttttttt gggctagcta ctagaggagg tactagatga cggtctgcgc aaaaaaacac 1981 gttcatctca ctcgcgatgc tgcggagcag ttactggctg atattgatcg acgccttgat 2041 cagttattgc ccgtggaggg agaacgggat gttgtgggtg ccgcgatgcg tgaaggtgcg 2101 ctggcaccgg gaaaacgtat tcgccccatg ttgctgttgc tgaccgcccg cgatctgggt 2161 tgcgctgtca gccatgacgg attactggat ttggcctgtg cggtggaaat ggtccacgcg 2221 gcttcgctga tccttgacga tatgccctgc atggacgatg cgaagctgcg gcgcggacgc 2281 cctaccattc attctcatta cggagagcat gtggcaatac tggcggcggt tgccttgctg 2341 agtaaagcct ttggcgtaat tgccgatgca gatggcctca cgccgctggc aaaaaatcgg 2401 gcggtttctg aactgtcaaa cgccatcggc atgcaaggat tggttcaggg tcagttcaag 2461 gatctgtctg aaggggataa gccgcgcagc gctgaagcta ttttgatgac gaatcacttt 2521 aaaaccagca cgctgttttg tgcctccatg cagatggcct cgattgttgc gaatgcctcc 2581 agcgaagcgc gtgattgcct gcatcgtttt tcacttgatc ttggtcaggc atttcaactg 2641 ctggacgatt tgaccgatgg catgaccgac accggtaagg atagcaatca ggacgccggt 2701 aaatcgacgc tggtcaatct gttaggcccg agggcggttg aagaacgtct gagacaacat 2761 cttcagcttg ccagtgagca tctctctgcg gcctgccaac acgggcacgc cactcaacat 2821 tttattcagg cctggtttga caaaaaactc gctgccgtca gttaataata ctagagctca 2881 aggaggtact agatgaataa tccgtcgtta ctcaatcatg cggtcgaaac gatggcagtt 2941 ggctcgaaaa gttttgcgac agcctcaaag ttatttgatg caaaaacccg gcgcagcgta 3001 ctgatgctct acgcctggtg ccgccattgt gacgatgtta ttgacgatca gacgctgggc 3061 tttcaggccc ggcagcctgc cttacaaacg cccgaacaac gtctgatgca acttgagatg 3121 aaaacgcgcc aggcctatgc aggatcgcag atgcacgaac cggcgtttgc ggcttttcag 3181 gaagtggcta tggctcatga tatcgccccg gcttacgcgt ttgatcatct ggaaggcttc 3241 gccatggatg tacgcgaagc gcaatacagc caactggatg atacgctgcg ctattgctat 3301 cacgttgcag gcgttgtcgg cttgatgatg gcgcaaatca tgggcgtgcg ggataacgcc 3361 acgctggacc gcgcctgtga ccttgggctg gcatttcagt tgaccaatat tgctcgcgat 3421 attgtggacg atgcgcatgc gggccgctgt tatctgccgg caagctggct ggagcatgaa 3481 ggtctgaaca aagagaatta tgcggcacct gaaaaccgtc aggcgctgag ccgtatcgcc 3541 cgtcgtttgg tgcaggaagc agaaccttac tatttgtctg ccacagccgg cctggcaggg 3601 ttgcccctgc gttccgcctg ggcaatcgct acggcgaagc aggtttaccg gaaaataggt 3661 gtcaaagttg aacaggccgg tcagcaagcc tgggatcagc ggcagtcaac gaccacgccc 3721 gaaaaattaa cgctgctgct ggccgcctct ggtcaggccc ttacttcccg gatgcgggct 3781 catcctcccc gccctgcgca tctctggcag cgcccgctct aataatacta gagctcaagg 3841 aggtactaga tgaaaccaac tacggtaatt ggtgcaggct tcggtggcct ggcactggca 3901 attcgtctac aagctgcggg gatccccgtc ttactgcttg aacaacgtga taaacccggc 3961 ggtcgggctt atgtctacga ggatcagggg tttacctttg atgcaggccc gacggttatc 4021 accgatccca gtgccattga agaactgttt gcactggcag gaaaacagtt aaaagagtat 4081 gtcgaactgc tgccggttac gccgttttac cgcctgtgtt gggagtcagg gaaggtcttt 4141 aattacgata acgatcaaac ccggctcgaa gcgcagattc agcagtttaa tccccgcgat 4201 gtcgaaggtt atcgtcagtt tctggactat tcacgcgcgg tgtttaaaga aggctatcta 4261 aagctcggta ctgtcccttt tttatcgttc agagacatgc ttcgcgccgc acctcaactg 4321 gcgaaactgc aagcatggag aagcgtttac agtaaggttg ccagttacat cgaagatgaa 4381 catctgcgcc aggcgttttc tttccactcg ctgttggtgg gcggcaatcc cttcgccacc 4441 tcatccattt atacgttgat acacgcgctg gagcgtgagt ggggcgtctg gtttccgcgt 4501 ggcggcaccg gcgcattagt tcaggggatg ataaagctgt ttcaggatct gggtggcgaa 4561 gtcgtgttaa acgccagagt cagccatatg gaaacgacag gaaacaagat tgaagccgtg 4621 catttagagg acggtcgcag gttcctgacg caagccgtcg cgtcaaatgc agatgtggtt 4681 catacctatc gcgacctgtt aagccagcac cctgccgcgg ttaagcagtc caacaaactg 4741 caaactaagc gcatgagtaa ctctctgttt gtgctctatt ttggtttgaa tcaccatcat 4801 gatcagctcg cgcatcacac ggtttgtttc ggcccgcgtt accgcgagct gattgacgaa 4861 atttttaatc atgatggcct cgcagaggac ttctcacttt atctgcacgc gccctgtgtc 4921 acggattcgt cactggcgcc tgaaggttgc ggcagttact atgtgttggc gccggtgccg 4981 catttaggca ccgcgaacct cgactggacg gttgaggggc caaaactacg cgaccgtatt 5041 tttgcgtacc ttgagcagca ttacatgcct ggcttacgga gtcagctggt cacgcaccgg 5101 atgtttacgc cgtttgattt tcgcgaccag cttaatgcct atcatggctc agccttttct 5161 gtggagcccg ttcttaccca gagcgcctgg tttcggccgc ataaccgcga taaaaccatt 5221 actaatctct acctggtcgg cgcaggcacg catcccggcg caggcattcc tggcgtcatc 5281 ggctcggcaa aagcgacagc aggtttgatg ctggaggatc tgatataata atactagagg 5341 aggtactaga tgcaaccgca ttatgatctg attctcgtgg gggctggact cgcgaatggc 5401 cttatcgccc tgcgtcttca gcagcagcaa cctgatatgc gtattttgct tatcgacgcc 5461 gcaccccagg cgggcgggaa tcatacgtgg tcatttcacc acgatgattt gactgagagc 5521 caacatcgtt ggatagctcc gctggtggtt catcactggc ccgactatca ggtacgcttt 5581 cccacacgcc gtcgtaagct gaacagcggc tacttttgta ttacttctca gcgtttcgct 5641 gaggttttac agcgacagtt tggcccgcac ttgtggatgg ataccgcggt cgcagaggtt 5701 aatgcggaat ctgttcggtt gaaaaagggt caggttatcg gtgcccgcgc ggtgattgac 5761 gggcggggtt atgcggcaaa ttcagcactg agcgtgggct tccaggcgtt tattggccag 5821 gaatggcgat tgagccaccc gcatggttta tcgtctccca ttatcatgga tgccacggtc 5881 gatcagcaaa atggttatcg cttcgtgtac agcctgccgc tctcgccgac cagattgtta 5941 attgaagaca cgcactatat tgataatgcg acattagatc ctgaatgcgc gcggcaaaat 6001 atttgcgact atgccgcgca acagggttgg cagcttcaga cactgctgcg agaagaacag 6061 ggcgccttac ccattactct gtcgggcaat gccgacgcat tctggcagca gcgccccctg 6121 gcctgtagtg gattacgtgc cggtctgttc catcctacca ccggctattc actgccgctg 6181 gcggttgccg tggccgaccg cctgagtgca cttgatgtct ttacgtcggc ctcaattcac 6241 catgccatta cgcattttgc ccgcgagcgc tggcagcagc agggcttttt ccgcatgctg 6301 aatcgcatgc tgtttttagc cggacccgcc gattcacgct ggcgggttat gcagcgtttt 6361 tatggtttac ctgaagattt aattgcccgt ttttatgcgg gaaaactcac gctgaccgat 6421 cggctacgta ttctgagcgg caagccgcct gttccggtat tagcagcatt gcaagccatt 6481 atgacgactc atcgttaata a //