BBa_K1780008
1
BBa_K1780008
Hybrid Promoter + RBS
2015-09-16T11:00:00Z
2015-09-17T10:28:04Z
From previous biobricks
This part has a strong RBS (BBa_B0034) downstream of the hybrid promoter placI/ara-1.
false
false
_2205_
26096
26096
9
false
This construct was necessary as the hybrid promoter did not have any RBS in its sequence already.
false
Snehal Kadam
component2466364
1
BBa_B0034
component2466362
1
BBa_K094120
annotation2466362
1
BBa_K094120
range2466362
1
1
103
annotation2466364
1
BBa_B0034
range2466364
1
112
123
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K094120
1
BBa_K094120
pLacI/ara-1
2008-10-12T11:00:00Z
2015-05-08T01:08:40Z
Standard pLacI/ara-1 promoter
This promoter can be repressed by lacI protein and induced by either IPTG or arabinose.
false
false
_255_
0
2589
9
It's complicated
false
IPTG has better effect on this promoter than arabinose
false
Shi Lei
annotation1980631
1
pLac/ara-1
range1980631
1
1
103
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1780008_sequence
1
catagcatttttatccataagattagcggatctaacctttacaattgtgagcgctcacaattatgatagattcaattgtgagcggataacaatttcacacagatactagagaaagaggagaaa
BBa_K094120_sequence
1
catagcatttttatccataagattagcggatctaacctttacaattgtgagcgctcacaattatgatagattcaattgtgagcggataacaatttcacacaga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z