LOCUS       BBa_K1780013            1797 bp    DNA     linear       16-Jun-2024
DEFINITION  c1 GFP system
ACCESSION   BBa_K1780013
VERSION     BBa_K1780013.1  
FEATURES             Location/Qualifiers
     promoter        1..86
                     /label=promoter
     promoter        1..86
                     /label=BBa_K1780010
     RBS             95..109
                     /label=RBS-1\Strong
     RBS             102..106
                     /label=RBS
     misc_feature    95..109
                     /label=BBa_B0030
     RBS             95..109
                     /label=BBa_B0030
     misc_feature    116..934
                     /label=misc
     CDS             116..934
                     /label=BBa_K1780011
     CDS             941..1660
                     /label=GFP protein
     CDS             941..1660
                     /label=BBa_E0040
     stem_loop       1680..1723
                     /label=stem_loop
     misc_feature    1669..1748
                     /label=BBa_B0010
     terminator      1669..1748
                     /label=BBa_B0010
     stem_loop       1764..1783
                     /label=T7 TE
     misc_feature    1790..1790
                     /label=stop
     polyA_site      1784..1797
                     /label=polya
     misc_feature    1757..1797
                     /label=BBa_B0012
     terminator      1757..1797
                     /label=BBa_B0012
     terminator      1669..1797
                     /label=BBa_B0015
ORIGIN
        1 atacgttaaa tctatcaccg caagggataa atatctaaca ccgtgcgtgt tgactatttt
       61 acctctggcg gtgataatgg ttgcattact agagattaaa gaggagaaat actagatgaa
      121 ctatgacgac ccaaaaaaga aggttggccg aaacatcaag cgattgaggt taaaagctgg
      181 cattaaaagt caagcggcct tggcggagct ttgcggctgg aagtcacaat caagagtcgg
      241 taactatgag gctggtacca gagcagtaag cgctattgat gctgagactc tggcgaaagt
      301 actcaacgta acacctgctg agatcctcta tggtgaaaac aataaagatg atgactttgt
      361 tagcatgcca agtgctcaat attcatcagg accatacgaa gaagaagatc cgtttcaact
      421 atttaaacca aaacaagaat tacaaaatgc tgagtggcat gcaggatttg agctatggga
      481 tggcgataca ccattaaggg atgatgaagt ggcactaccg ttttaccgtg aagtggaatt
      541 agccgcaggc agtggatcaa cctttgtgca agaaaacggc ggatgcaagc tacgctttgc
      601 taaatcaacc ctcaaaaaaa gccgagtaga accaagcaat gcggcctgtg tgaccgtaag
      661 cggcaatagc atgctgccag tactgcgcca cggcaccaca gttggtgtcg ataccagtaa
      721 aaaatcgatt atcgatggtg agatgtatgc cattgatcac gatggcatgt tgcgagtaaa
      781 aatgctctat cgcaccccag gcggtggcat tcgcattaag agctataaca atgatgaatt
      841 ccccgatgaa tttatcccac cagaaaaagc atccgatatt aaaattatcg gttgggtgtt
      901 ttggtggtca gttctaaacg tttggaataa ttgatactag atgcgtaaag gagaagaact
      961 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
     1021 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
     1081 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg
     1141 tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc
     1201 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
     1261 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
     1321 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
     1381 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat
     1441 tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc
     1501 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct
     1561 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc
     1621 tgggattaca catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa
     1681 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1741 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//