LOCUS       BBa_K1780014            1587 bp    DNA     linear       22-Nov-2024
DEFINITION  rcs-mRFP system
ACCESSION   BBa_K1780014
VERSION     BBa_K1780014.1  
FEATURES             Location/Qualifiers
     promoter        1..86
                     /label=promoter
     promoter        1..86
                     /label=BBa_K1780010
     misc_feature    101..104
                     /label=conserved
     RBS             95..108
                     /label=BBa_B0031
     misc_feature    115..738
                     /label=misc
     CDS             115..738
                     /label=BBa_K1780012
     CDS             745..1419
                     /label=mrfp1
     misc_feature    1426..1450
                     /label=Help:Barcodes
     CDS             745..1450
                     /label=BBa_E1010
     stem_loop       1470..1513
                     /label=stem_loop
     misc_feature    1459..1538
                     /label=BBa_B0010
     terminator      1459..1538
                     /label=BBa_B0010
     stem_loop       1554..1573
                     /label=T7 TE
     misc_feature    1580..1580
                     /label=stop
     polyA_site      1574..1587
                     /label=polya
     misc_feature    1547..1587
                     /label=BBa_B0012
     terminator      1547..1587
                     /label=BBa_B0012
     terminator      1459..1587
                     /label=BBa_B0015
ORIGIN
        1 atacgttaaa tctatcaccg caagggataa atatctaaca ccgtgcgtgt tgactatttt
       61 acctctggcg gtgataatgg ttgcattact agagtcacac aggaaaccta ctagatgtca
      121 acgattatta tggatttatg tagttacacc cgactaggtt taaccgggta tctgttgagt
      181 agaggggtta aaaaaagaga aatcaacgac attgaaaccg ttgatgacct tgccatagct
      241 tgtgattcac agcgcccttc agtggtgttt attaatgagg actgtttcat ccacgatgct
      301 tctaacagtc agcgtatcaa gctcatcatt aatcaacatc ccaatacgtt atttatcgtt
      361 tttatggcaa ttgccaatgt tcattttgat gaatatctat tggtcagaaa aaatttattg
      421 atcagttcta aatcgattaa accggaatct ctcgacgata tccttggcga tattctgaaa
      481 aaagagacaa cgataacctc gtttttaaat atgccgacgt tatcattgag ccgaaccgaa
      541 tcgagtatgt tgcgaatgtg gatggcaggt cagggaacca ttcaaatctc tgaccaaatg
      601 aatatcaaag ccaagaccgt ttcatcgcat aaaggtaata ttaaacgtaa gatcaaaacg
      661 cataataaac aggttatcta ccatgtcgtc cgactgacgg ataatgtgac taatggtatt
      721 tttgtcaaca tgcgctaata ctagatggct tcctccgaag acgttatcaa agagttcatg
      781 cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa
      841 ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt
      901 ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac
      961 gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa
     1021 tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc
     1081 ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac
     1141 ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg
     1201 gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac
     1261 tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct
     1321 tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa
     1381 cagtacgaac gtgctgaagg tcgtcactcc accggtgctt aataacgctg atagtgctag
     1441 tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1501 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1561 cttcgggtgg gcctttctgc gtttata
//