##gff-version 3
##sequence-region BBa_K1780017 1 1901
BBa_K1780017	.	ribosome_entry_site	1070	1081	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1780017	.	conserved_region	1074	1077	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1780017	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1780017	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1780017	.	terminator	870	998	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1780017	.	terminator	870	949	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1780017	.	stem_loop	881	924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1780017	.	engineered_region	870	949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1780017	.	terminator	958	998	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1780017	.	polyA_site	985	998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1780017	.	stem_loop	965	984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1780017	.	stop_codon	991	991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1780017	.	engineered_region	958	998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1780017	.	promoter	1007	1061	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K1780017	.	promoter	1026	1031	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1780017	.	engineered_region	1007	1061	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1780017	.	promoter	1048	1053	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1780017	.	start_codon	1059	1059	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1780017	.	non_covalent_binding_site	1007	1026	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1780017	.	engineered_region	1090	1764	.	+	0	ID=BBa_P1010;Name=BBa_P1010
BBa_K1780017	.	sequence_feature	1707	1712	.	+	0	ID=annotation1747336;Name=-35 (rev);Parent=BBa_P1010
BBa_K1780017	.	point_mutation	1556	1556	.	+	0	ID=annotation1747334;Name=T to C mutation;Parent=BBa_P1010
BBa_K1780017	.	CDS	1118	1423	.	+	0	ID=annotation1747332;Name=ccdB (rev);Parent=BBa_P1010
BBa_K1780017	.	sequence_feature	1684	1689	.	+	0	ID=annotation1747335;Name=-10 (rev);Parent=BBa_P1010
BBa_K1780017	.	CDS	1425	1645	.	+	0	ID=annotation1747333;Name=ccdA- (rev);Parent=BBa_P1010
BBa_K1780017	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1780017	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1780017	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1780017	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1780017	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1780017	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1780017	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K1780017	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1780017	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1780017	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1780017	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1780017	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1780017	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1780017	.	terminator	1773	1901	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1780017	.	terminator	1773	1852	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1780017	.	stem_loop	1784	1827	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1780017	.	engineered_region	1773	1852	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1780017	.	terminator	1861	1901	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1780017	.	polyA_site	1888	1901	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1780017	.	stem_loop	1868	1887	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1780017	.	stop_codon	1894	1894	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1780017	.	engineered_region	1861	1901	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K1780017
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagagactggctgtgtataagg
gagcctgacatttatattccccagaacatcaggttaatggcgtttttgatgtcattttcgcggtggctgagatcagcca
cttcttccccgataacggagaccggcacactggccatatcggtggtcatcatgcgccagctttcatccccgatatgcac
caccgggtaaagttcacgggagactttatctgacagcagacgtgcactggccagggggatcaccatccgtcgcccgggc
gtgtcaataatatcactctgtacatccacaaacagacgataacggctctctcttttataggtgtaaaccttaaactgca
tttcaccagcccctgttctcgtcagcaaaagagccgttcatttcaataaaccgggcgacctcagccatcccttcctgat
tttccgctttccagcgttcggcacgcagacgacgggcttcattctgcatggttgtgcttaccagaccggagatattgac
atcatatatgccttgagcaactgatagctgtcgctgtcaactgtcactgtaatacgctgcttcatagcatacctctttt
tgacatacttcgggtatacatatcagtatatattcttataccgcaaaaatcagcgcgcaaatacgcatactgttatctg
gcttttagtaagccggatccacgcgttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct
ttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgt
ttata