LOCUS BBa_K1780018 2770 bp DNA linear 25-Nov-2024 DEFINITION Bacterial population growth control device ACCESSION BBa_K1780018 VERSION BBa_K1780018.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 661..692 /label=LVA CDS 82..660 /label=luxI misc_feature 82..699 /label=BBa_C0061 misc_feature 700..724 /label=Help:Barcodes CDS 82..699 /label=BBa_C0061 stem_loop 744..787 /label=stem_loop misc_feature 733..812 /label=BBa_B0010 terminator 733..812 /label=BBa_B0010 stem_loop 828..847 /label=T7 TE misc_feature 854..854 /label=stop polyA_site 848..861 /label=polya misc_feature 821..861 /label=BBa_B0012 terminator 821..861 /label=BBa_B0012 terminator 733..861 /label=BBa_B0015 misc_feature 870..888 /label=TetR 1 misc_feature 870..923 /label=BBa_R0040 promoter 889..894 /label=-35 misc_feature 895..913 /label=TetR 2 promoter 912..917 /label=-10 promoter 870..923 /label=BBa_R0040 misc_feature 936..939 /label=conserved RBS 932..943 /label=BBa_B0034 misc_feature 950..951 /label=prefix misc_feature 1123..1123 /label=T misc_feature 1441..1441 /label=A CDS 950..1699 /label=luxR misc_feature 950..1705 /label=BBa_C0062 misc_feature 1706..1730 /label=Help:Barcodes CDS 950..1705 /label=BBa_C0062 stem_loop 1750..1793 /label=stem_loop misc_feature 1739..1818 /label=BBa_B0010 terminator 1739..1818 /label=BBa_B0010 stem_loop 1834..1853 /label=T7 TE misc_feature 1860..1860 /label=stop polyA_site 1854..1867 /label=polya misc_feature 1827..1867 /label=BBa_B0012 terminator 1827..1867 /label=BBa_B0012 terminator 1739..1867 /label=BBa_B0015 misc_feature 1876..1895 /label=LuxR/HSL promoter 1895..1900 /label=-35 promoter 1917..1922 /label=-10 misc_feature 1928..1928 /label=start misc_feature 1876..1930 /label=BBa_R0062 promoter 1876..1930 /label=BBa_R0062 misc_feature 1943..1946 /label=conserved RBS 1939..1950 /label=BBa_B0034 CDS 1987..2292 /label=ccdB (rev) CDS 2294..2514 /label=ccdA- (rev) misc_feature 2425..2425 /label=T to C mutation misc_feature 2553..2558 /label=-10 (rev) misc_feature 2576..2581 /label=-35 (rev) misc_feature 1959..2633 /label=BBa_P1010 stem_loop 2653..2696 /label=stem_loop misc_feature 2642..2721 /label=BBa_B0010 terminator 2642..2721 /label=BBa_B0010 stem_loop 2737..2756 /label=T7 TE misc_feature 2763..2763 /label=stop polyA_site 2757..2770 /label=polya misc_feature 2730..2770 /label=BBa_B0012 terminator 2730..2770 /label=BBa_B0012 terminator 2642..2770 /label=BBa_B0015 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt 121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt 181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca 241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct 301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct 361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag 421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct 481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta 541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat 601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat 661 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga 721 tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 781 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 841 gtgggccttt ctgcgtttat atactagagt ccctatcagt gatagagatt gacatcccta 901 tcagtgatag agatactgag cactactaga gaaagaggag aaatactaga tgaaaaacat 961 aaatgccgac gacacataca gaataattaa taaaattaaa gcttgtagaa gcaataatga 1021 tattaatcaa tgcttatctg atatgactaa aatggtacat tgtgaatatt atttactcgc 1081 gatcatttat cctcattcta tggttaaatc tgatatttca atcctagata attaccctaa 1141 aaaatggagg caatattatg atgacgctaa tttaataaaa tatgatccta tagtagatta 1201 ttctaactcc aatcattcac caattaattg gaatatattt gaaaacaatg ctgtaaataa 1261 aaaatctcca aatgtaatta aagaagcgaa aacatcaggt cttatcactg ggtttagttt 1321 ccctattcat acggctaaca atggcttcgg aatgcttagt tttgcacatt cagaaaaaga 1381 caactatata gatagtttat ttttacatgc gtgtatgaac ataccattaa ttgttccttc 1441 tctagttgat aattatcgaa aaataaatat agcaaataat aaatcaaaca acgatttaac 1501 caaaagagaa aaagaatgtt tagcgtgggc atgcgaagga aaaagctctt gggatatttc 1561 aaaaatatta ggttgcagtg agcgtactgt cactttccat ttaaccaatg cgcaaatgaa 1621 actcaataca acaaaccgct gccaaagtat ttctaaagca attttaacag gagcaattga 1681 ttgcccatac tttaaaaatt aataacactg atagtgctag tgtagatcac tactagagcc 1741 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 1801 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 1861 gtttatatac tagagacctg taggatcgta caggtttacg caagaaaatg gtttgttata 1921 gtcgaataaa tactagagaa agaggagaaa tactagagac tggctgtgta taagggagcc 1981 tgacatttat attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg 2041 gctgagatca gccacttctt ccccgataac ggagaccggc acactggcca tatcggtggt 2101 catcatgcgc cagctttcat ccccgatatg caccaccggg taaagttcac gggagacttt 2161 atctgacagc agacgtgcac tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat 2221 aatatcactc tgtacatcca caaacagacg ataacggctc tctcttttat aggtgtaaac 2281 cttaaactgc atttcaccag cccctgttct cgtcagcaaa agagccgttc atttcaataa 2341 accgggcgac ctcagccatc ccttcctgat tttccgcttt ccagcgttcg gcacgcagac 2401 gacgggcttc attctgcatg gttgtgctta ccagaccgga gatattgaca tcatatatgc 2461 cttgagcaac tgatagctgt cgctgtcaac tgtcactgta atacgctgct tcatagcata 2521 cctctttttg acatacttcg ggtatacata tcagtatata ttcttatacc gcaaaaatca 2581 gcgcgcaaat acgcatactg ttatctggct tttagtaagc cggatccacg cgttactaga 2641 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 2701 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 2761 tgcgtttata //