##gff-version 3
##sequence-region BBa_K1780018 1 2770
BBa_K1780018	.	ribosome_entry_site	932	943	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1780018	.	conserved_region	936	939	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1780018	.	promoter	1876	1930	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K1780018	.	promoter	1917	1922	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1780018	.	engineered_region	1876	1930	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1780018	.	promoter	1895	1900	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1780018	.	start_codon	1928	1928	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1780018	.	non_covalent_binding_site	1876	1895	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1780018	.	promoter	870	923	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1780018	.	promoter	889	894	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1780018	.	non_covalent_binding_site	895	913	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1780018	.	engineered_region	870	923	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1780018	.	promoter	912	917	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1780018	.	non_covalent_binding_site	870	888	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1780018	.	engineered_region	1959	2633	.	+	0	ID=BBa_P1010;Name=BBa_P1010
BBa_K1780018	.	sequence_feature	2576	2581	.	+	0	ID=annotation1747336;Name=-35 (rev);Parent=BBa_P1010
BBa_K1780018	.	point_mutation	2425	2425	.	+	0	ID=annotation1747334;Name=T to C mutation;Parent=BBa_P1010
BBa_K1780018	.	CDS	1987	2292	.	+	0	ID=annotation1747332;Name=ccdB (rev);Parent=BBa_P1010
BBa_K1780018	.	sequence_feature	2553	2558	.	+	0	ID=annotation1747335;Name=-10 (rev);Parent=BBa_P1010
BBa_K1780018	.	CDS	2294	2514	.	+	0	ID=annotation1747333;Name=ccdA- (rev);Parent=BBa_P1010
BBa_K1780018	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1780018	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1780018	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K1780018	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1780018	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1780018	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1780018	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1780018	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1780018	.	ribosome_entry_site	1939	1950	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1780018	.	conserved_region	1943	1946	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1780018	.	terminator	2642	2770	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1780018	.	terminator	2642	2721	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1780018	.	engineered_region	2642	2721	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1780018	.	stem_loop	2653	2696	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1780018	.	terminator	2730	2770	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1780018	.	engineered_region	2730	2770	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1780018	.	stop_codon	2763	2763	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1780018	.	stem_loop	2737	2756	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1780018	.	polyA_site	2757	2770	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1780018	.	CDS	82	699	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K1780018	.	CDS	82	660	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1780018	.	engineered_tag	700	724	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1780018	.	engineered_region	82	699	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1780018	.	tag	661	692	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1780018	.	terminator	733	861	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1780018	.	terminator	733	812	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1780018	.	engineered_region	733	812	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1780018	.	stem_loop	744	787	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1780018	.	terminator	821	861	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1780018	.	engineered_region	821	861	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1780018	.	stop_codon	854	854	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1780018	.	stem_loop	828	847	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1780018	.	polyA_site	848	861	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1780018	.	CDS	950	1705	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K1780018	.	point_mutation	1441	1441	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1780018	.	CDS	950	1699	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1780018	.	engineered_region	950	1705	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1780018	.	engineered_tag	1706	1730	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1780018	.	point_mutation	1123	1123	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1780018	.	engineered_region	950	951	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1780018	.	terminator	1739	1867	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1780018	.	terminator	1739	1818	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1780018	.	engineered_region	1739	1818	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1780018	.	stem_loop	1750	1793	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1780018	.	terminator	1827	1867	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1780018	.	engineered_region	1827	1867	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1780018	.	stop_codon	1860	1860	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1780018	.	stem_loop	1834	1853	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1780018	.	polyA_site	1854	1867	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K1780018
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtta
tcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaat
gcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattata
tgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttt
tgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaa
cacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaag
ttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaa
tgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgc
tagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagagactggctgtgtataagg
gagcctgacatttatattccccagaacatcaggttaatggcgtttttgatgtcattttcgcggtggctgagatcagcca
cttcttccccgataacggagaccggcacactggccatatcggtggtcatcatgcgccagctttcatccccgatatgcac
caccgggtaaagttcacgggagactttatctgacagcagacgtgcactggccagggggatcaccatccgtcgcccgggc
gtgtcaataatatcactctgtacatccacaaacagacgataacggctctctcttttataggtgtaaaccttaaactgca
tttcaccagcccctgttctcgtcagcaaaagagccgttcatttcaataaaccgggcgacctcagccatcccttcctgat
tttccgctttccagcgttcggcacgcagacgacgggcttcattctgcatggttgtgcttaccagaccggagatattgac
atcatatatgccttgagcaactgatagctgtcgctgtcaactgtcactgtaatacgctgcttcatagcatacctctttt
tgacatacttcgggtatacatatcagtatatattcttataccgcaaaaatcagcgcgcaaatacgcatactgttatctg
gcttttagtaagccggatccacgcgttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct
ttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgt
ttata