BBa_K1783000 1 BBa_K1783000 Miraculin Generator 2015-06-02T11:00:00Z 2015-07-01T08:39:40Z All parts are from previous BioBricks. pBAD promoter is from E. coli, while Miraculin gene comes from S. dulcificum. pBAD-B0034-ExpTag-Linker-Miraculin-Linker-6xHis. Adds a promoter to BBa_K1033120 in order to characterize it. Under arabinose induction, produces miraculin connected to an export tag and a His-tag via unstructured linkers. Miraculin is a taste modifying protein. false false _2208_ 20382 20382 9 false Parts were assembled using 3A assembly. false Iowis Zhu component2432926 1 BBa_K206000 component2432933 1 BBa_K1033120 annotation2432933 1 BBa_K1033120 range2432933 1 139 855 annotation2432926 1 BBa_K206000 range2432926 1 1 130 BBa_K206000 1 pBAD pBAD strong 2009-10-13T11:00:00Z 2015-05-08T01:11:23Z The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1]. Released HQ 2013 pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter. false false _307_ 0 4172 9 In stock true There were no special design considerations. false Amelia Hardjasa annotation2049254 1 AraI2 range2049254 1 61 78 annotation2049253 1 AraI1 range2049253 1 40 57 annotation2049252 1 promoter range2049252 1 1 131 BBa_K1033120 1 BBa_K1033120 B0034-ExpTag-Linker-Miraculin-Linker-6xHis 2013-09-01T11:00:00Z 2015-06-11T08:28:53Z Richadella Dulcifica, but codon optimized for E.coli only the miraculin is codon optimized. The codon usage for the export tag USP45's is best suited for Lactobacillus strains Construct needed to produce the protein miraculin, but fitted with a, export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes. false false _1340_ 4206 18316 9 In stock false The miraculin sequence is codon optimized for E.coli. The codon usage for the export tag USP45's is best suited for Lactobacillus strains false Magnus Bergman, Pontus Danielsson, Alexander Wirtanen, Nils Anlind, Christoffer Frisk, Thorsteinn Olaufsson, Malin Borg, Niclas Sandberg annotation2334587 1 B0034 range2334587 1 1 12 annotation2340530 1 Linker range2340530 1 682 690 annotation2340531 1 Linker range2340531 1 100 108 annotation2334586 1 USP45 range2334586 1 18 99 annotation2340533 1 Miraculin range2340533 1 109 681 annotation2340532 1 His-tag range2340532 1 691 714 BBa_K1783000_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagtataaagaggagaaatatacgatgaaaaaaaagattatctcagctattttaatgtctacagtgatactttctgctgctgcaccgttgtcaggtgtttacgctagtggaagtgattcagcccctaacccggtgttggatattgatggagaaaaattacgcacgggaactaactattacattgtaccagtattgcgcgatcatggtggcggacttacagtgagtgcgactaccccaaatggcacgtttgtatgtccaccgcgcgttgtgcaaacacgtaaagaagttgatcacgatcgcccactggctttctttccggaaaaccctaaggaagatgtagttcgtgtgagtactgatttaaacattaacttcagtgcttttatgccatgccgctggacaagttcaacggtttggcgtttggataagtacgatgaaagtactggtcaatactttgtgaccattggtggcgtaaaaggcaatcctggaccagaaactattagctcttggttcaagattgaagaattttgtggaagcggtttttataagttagttttctgccctaccgtgtgtggatcttgcaaagtaaagtgtggtgatgttggcatttacattgatcagaaaggtcgtcgccgtctggctttatcagataagccgtttgcctttgaatttaataagactgtttacttctcaggttctcatcaccatcatcaccattaataa BBa_K1033120_sequence 1 tataaagaggagaaatatacgatgaaaaaaaagattatctcagctattttaatgtctacagtgatactttctgctgctgcaccgttgtcaggtgtttacgctagtggaagtgattcagcccctaacccggtgttggatattgatggagaaaaattacgcacgggaactaactattacattgtaccagtattgcgcgatcatggtggcggacttacagtgagtgcgactaccccaaatggcacgtttgtatgtccaccgcgcgttgtgcaaacacgtaaagaagttgatcacgatcgcccactggctttctttccggaaaaccctaaggaagatgtagttcgtgtgagtactgatttaaacattaacttcagtgcttttatgccatgccgctggacaagttcaacggtttggcgtttggataagtacgatgaaagtactggtcaatactttgtgaccattggtggcgtaaaaggcaatcctggaccagaaactattagctcttggttcaagattgaagaattttgtggaagcggtttttataagttagttttctgccctaccgtgtgtggatcttgcaaagtaaagtgtggtgatgttggcatttacattgatcagaaaggtcgtcgccgtctggctttatcagataagccgtttgcctttgaatttaataagactgtttacttctcaggttctcatcaccatcatcaccattaataa BBa_K206000_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z