BBa_K1789000 1 BBa_K1789000 IaaM 2015-09-11T11:00:00Z 2015-09-12T02:27:07Z This part is gained from BBa_K515100 by PCR. The IAA producing pathway originates from Pseudomonas savastanoi. This part is the coding sequence of tryptophan-2-mono-oxygenase. This enzyme catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which acts as an intermediate to the production of indole-3-acetic acid in the IAM pathway. false false _2214_ 21134 21134 9 false The reason why er designed this part is that we needed IAA producing pathway as a reporter in our system, and the IAAM and IAAH CDS needed to be fused with the coding sequence of the TAL effector we engineered. So we used the part BBa_K515100, which contains the CDS of IaaM and IaaH, and used PCR to eliminate the RBS sequence from the 5' region of the CDS. false Xinyuan Qiu annotation2452420 1 IaaM range2452420 1 1 1638 BBa_K1789000_sequence 1 atgtttggaccggatttcccgtttgcatttgatgattggatcgaacatcctaaaggacttgggtcaattccggcagaaaatcacggcgctgaggttgcaattatcggggccggcattgcaggcttagtagcagcatatgaattaatgaagatgggtctgaaacctgtagtctatgaggcctccaaaatgggaggtcgcttacggtcacaggaatttgaaggagcgaagggaattgttgcggaactgggcggtatgcgctttccagtctcctccacagcgttctttcattacgttgataaactgggccttgaaagtcgtccgttccctaaccctttgacggcggccagtggcagcacggtgattgacctggaaggtaccacgtactatgcacagatgttatcagatctcccagccctgttccaagaagtggcggatgcatgggcagacgccttagaatcaggctctcaatttggagatattcaacaggccattcgtgattctgacgtaccgcgtctcaaggaactgtggaacaaactcgtgcctttgtgggacgatcgcactttctatgactttgtagcgacctcaaaggcctttgcgaaattaagcttttaccatcgtgaggttttcggacaagttggttttggtacaggggggtgggattctgattttccaaatagtatgttagaaatctttcgtgttgtgatgacgaattgtgatgagcaccagcatctgattgttggtggcgtacagcaagtgcctgtaggcctgtggtcgcacgtgcctgaacgctgcgcacattggccaaagggtacgtctctttcctcacttcatcggggtgccccgcgcccaggtgtcaaacgtattgcacgtgctgaagatggttcatttacggttacagataattggggcgatacacgtcaatacgctgccgttttgacaacctgtcaatcgtggctgcttacaacgcagattgaatgtgaggaatctttgttttcacagaagatgtggatggcgctggatcgcacgcgttacatgcagtcttctaaaacttttgtgatggttgatcggccgttttggaaggacaaagatccggaaacgggtcgcgatcttatgtccatgaccctcaccgatcgcttaacgcgcggcacatacttatttgataatggcgacgacaagccaggcgttatttgcctgagttactcatggatgagtgacgctttaaaaatgttaccgcaaccgatcgagaaacgcgtcaaattagctttagatgcccttaaaaagatttacccgaaagtggatatcgccgcacgtattattggtgacccgattactgtgagttgggaagcggatccgcatttcctgggggcatttaaaggcgcgttaccgggccattaccgttacaaccagcgcatgtacgcgcattttatgcagcaggacatgccgtcagaacagcgtggcatgtttattgccggtgatgatgtatcttggacaccggcatgggttgagggagcagtacaaacgtcattgaacgcggtgtggggcatcatgaaccatttcggagggaaaacccacgcagagaatccgggtcctggcgacgttttccatgagattggaccgatcgcgctgggggactaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z