BBa_K1789001 1 BBa_K1789001 IaaH 2015-09-13T11:00:00Z 2015-09-14T05:49:28Z This part is gained from the part BBa_K515100 by PCR. This part is the coding sequence of the enzyme Indoleacetimide hydrolase Iaa H. This enzyme catalyses the hydrolysis of indoleacetamide to indoleacetate and ammonia. This part is gathered from the 2-step pathway in Pseudomonas savastanoi producing the auxin, indole-3-acetic acid. false false _2214_ 21134 21134 9 false In order to get the whole sequence of IAAH, we removed the RBS and other irrelevant sequences in K515100 when designing the PCR primers. false Xinyuan Qiu annotation2454035 1 IaaH range2454035 1 1 1335 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K1789011 1 BBa_K1789011 IAAH+Ter 2015-09-13T11:00:00Z 2015-09-18T10:46:33Z It is constructed by ourselves. It is the combination of IAAH and termination. false false _2214_ 21134 26298 9 false We combine IAAH and the termination to improve the efficiency of the building. false Jiaqi Sun component2454246 1 BBa_K1789001 component2454253 1 BBa_B0015 annotation2454253 1 BBa_B0015 range2454253 1 1344 1472 annotation2454246 1 BBa_K1789001 range2454246 1 1 1335 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1789001_sequence 1 atgattacgcttgaatctctgtgccgcgcattggcagacgaacagattgccgcagaagaattgcgcgagcgcgcgctggatacagaggctcgcttaacactgttaaattgcttcattcgcgaaggcgacgccgtaagccaattcggtgaagcggatcaggcgcgtaaaggaacttcactttggggggtgcctgtgagttttaaggataacatctgtgttcgcgggctgccactgacggcgggtacccgcggcatgagcggctttattgccgatcaggatgcagcaatcgtgagccagttaaaagcattgggagctgtggtcgcgggcaaaaataacatgcatgagctgtcatttggcgtcacgagcatcaatccacattggggcgcggttggaaacccggtggctccaggatattgcgccggaggttctagcggaggttccgctgcggcggtcgcatctggaattgtcccgctcagcgtcgggacggacacaggcggctcaatccgtattccggcggcattttgcggcattacgggttttcgccctactacaggtcgcttgagtacggcggggatcattccggtcagccacacgaaggattgtgtcggcctcctgacgcgcacagctggcgacgctgaatttgtgtatgggctgctgtcaggaaaacagcagagtttccctcttaatcgcacgggaccttgccgcatcggcctgccggtgagcatgtggtctgacttagatggcgaagtagaacgtgcctgtatcaatgcgctgagcctcttacgcaaaacgggctttgaatttgttgagatcgacgatgcggacattgtggaattgaaccagacattaaccttcacggtgccgctgtacgaatttttcgcggattttgctcaatccttgttgtccttaggttggaaacatggcatccatcatatttttgcacaagtggatgatgcaaatgtgaaaggcattattaatcaccatctgggagagggcgcgattaagccggcacattatctgagttctctccagaatggagagctgctgaaacgcaagatggatgagctttttgcgcgccatcacattaaacttttagggtatccgacggtcccgtgtcgcgtgcctcatctggaccatgcagatcgtccggaattttttagccaggccatccgtaacacagacctcgcaagcaacgcgatgttaccaagcattacaattccggtcggtccggaaggccgcctgccggtcggccttagttttgatgcgcctcgtgctcgtgatgcgtttctcttaagcaatgttagtttgatcgaaaaagtgttaaaaggctaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1789011_sequence 1 atgattacgcttgaatctctgtgccgcgcattggcagacgaacagattgccgcagaagaattgcgcgagcgcgcgctggatacagaggctcgcttaacactgttaaattgcttcattcgcgaaggcgacgccgtaagccaattcggtgaagcggatcaggcgcgtaaaggaacttcactttggggggtgcctgtgagttttaaggataacatctgtgttcgcgggctgccactgacggcgggtacccgcggcatgagcggctttattgccgatcaggatgcagcaatcgtgagccagttaaaagcattgggagctgtggtcgcgggcaaaaataacatgcatgagctgtcatttggcgtcacgagcatcaatccacattggggcgcggttggaaacccggtggctccaggatattgcgccggaggttctagcggaggttccgctgcggcggtcgcatctggaattgtcccgctcagcgtcgggacggacacaggcggctcaatccgtattccggcggcattttgcggcattacgggttttcgccctactacaggtcgcttgagtacggcggggatcattccggtcagccacacgaaggattgtgtcggcctcctgacgcgcacagctggcgacgctgaatttgtgtatgggctgctgtcaggaaaacagcagagtttccctcttaatcgcacgggaccttgccgcatcggcctgccggtgagcatgtggtctgacttagatggcgaagtagaacgtgcctgtatcaatgcgctgagcctcttacgcaaaacgggctttgaatttgttgagatcgacgatgcggacattgtggaattgaaccagacattaaccttcacggtgccgctgtacgaatttttcgcggattttgctcaatccttgttgtccttaggttggaaacatggcatccatcatatttttgcacaagtggatgatgcaaatgtgaaaggcattattaatcaccatctgggagagggcgcgattaagccggcacattatctgagttctctccagaatggagagctgctgaaacgcaagatggatgagctttttgcgcgccatcacattaaacttttagggtatccgacggtcccgtgtcgcgtgcctcatctggaccatgcagatcgtccggaattttttagccaggccatccgtaacacagacctcgcaagcaacgcgatgttaccaagcattacaattccggtcggtccggaaggccgcctgccggtcggccttagttttgatgcgcctcgtgctcgtgatgcgtttctcttaagcaatgttagtttgatcgaaaaagtgttaaaaggctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z