BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1789006 1 BBa_K1789006 SCAF2 2015-09-12T11:00:00Z 2015-09-18T08:33:02Z This part is synthetized by viewsolid biotech firm (Beijing, China). This part is a highly repetitive DNA sequence which can bind TALE protein specifically. false false _2214_ 21134 26299 9 false This part is designed to contain two basic short sequences which can bind TALE1 and TALE2(3) specifically. These two basic sequences are repeated with no space by ten times, so it can bind more TALE protein and the spatial location of each pair of TALE1 and TALE2(3) is almost opposite. false Xinyuan Qiu annotation2470918 1 BM2 range2470918 1 21 34 annotation2453186 1 SCAF2 range2453186 1 1 462 annotation2470907 1 BM1 range2470907 1 1 14 BBa_K1789014 1 BBa_K1789014 SCAF2+Ter 2015-09-13T11:00:00Z 2015-09-14T08:10:36Z It is constructed by our own basic parts. It is the combination of scaffold system and termination. false false _2214_ 26298 26298 9 false We design this part to add it into other building work. A composite can improve our efficiency. false Jiaqi Sun component2454262 1 BBa_K1789006 component2454260 1 BBa_B0015 annotation2454262 1 BBa_K1789006 range2454262 1 138 416 annotation2454260 1 BBa_B0015 range2454260 1 1 129 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1789014_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctagatggaggcaccggtggtgataaacacctttctggaggcaccggtggtgataaacacctttctggaggcaccggtggtgataaacacctttctggaggcaccggtggtgataaacacctttctggaggcaccggtggtgataaacacctttctggaggcaccggtggtgataaacacctttctggaggcaccggtggtgataaacacctttctggaggcaccggtggtgataaacacctttctggaggcaccggtggtgataaacacctttctgga BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1789006_sequence 1 tggaggcaccggtggacagtatgataaacacctttcacagtatggaggcaccggtggacagtatgataaacacctttcacagtatggaggcaccggtggacagtatgataaacacctttcacagtatggaggcaccggtggacagtatgataaacacctttcacagtatggaggcaccggtggacagtatgataaacacctttcacagtatggaggcaccggtggacagtatgataaacacctttcacagtatggaggcaccggtggacagtatgataaacacctttcacagtatggaggcaccggtggacagtatgataaacacctttcacagtatggaggcaccggtggacagtatgataaacacctttcacagtatggaggcaccggtggacagtatgataaacacctttcacagtatggaggcaccggtggacagtatgataaacacctttcacagta BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z