BBa_K1789003 1 BBa_K1789003 GFP1(with termination codon) 2015-09-13T11:00:00Z 2016-01-25T01:04:29Z This part is gained from part BBa_I715019. we added the termination codon when designing the PCR. This part is the Amino Half of GFP with termination codon false false _2214_ 4206 21134 9 false Because of the need of our project, we added a termination codon when designing the PCR. false Xinyuan Qiu annotation2454066 1 termination codon range2454066 1 472 474 annotation2454065 1 GFP1 range2454065 1 1 471 BBa_K1789009 1 BBa_K1789009 TALE2 2015-09-13T11:00:00Z 2015-09-14T07:20:22Z This part is built and standardized by ourselves using GATEWAY technique. This is a engineered TAL effector that can bind with the DNA sequence 5'-GATAAACACCTTTC-3' false false _2214_ 21134 21134 9 false The part sequence should be able to correctly and efficiently bind with corresponding DNA, so we used bioinformatics tools to help us designing this sequence. false Xinyuan Qiu annotation2454120 1 DNA Binding Domain range2454120 1 514 1872 annotation2454121 1 TALE2 range2454121 1 1 2088 BBa_K1789002 1 BBa_K1789002 RBS30 2015-09-13T11:00:00Z 2015-09-14T06:03:14Z The part sequence was gained from the registry, the part is annealed from the ssDNA synthesized by Life(R). This part is another copy of the RBS30 (BBa_B0030). It is a Strong RBS based on Ron Weiss thesis. false false _2214_ 21134 21134 9 false The reason why we built this part by ourselves is that the copy of B0030 in the 2015 DNA distribution we received is sequenced as incorrect. And same problem also occurred in several other teams. false Xinyuan Qiu annotation2454064 1 RBS30 range2454064 1 1 15 BBa_K1789004 1 BBa_K1789004 GFP2 2015-09-13T11:00:00Z 2016-01-25T01:04:48Z This part is gained from part BBa_I715020. This part is the Carboxyl Half of GFP false false _2214_ 4206 21134 9 false we REMOVED the extra base from part BBa_I715020(easier to fuse with other proteins) when designing PCR primers to make the protein fusion easier. false Xinyuan Qiu annotation2454067 1 GFP2 range2454067 1 1 249 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961224 1 -35 range1961224 1 137 142 annotation1961227 1 start range1961227 1 173 173 annotation1961225 1 -10 range1961225 1 161 166 BBa_K1789008 1 BBa_K1789008 TALE1 2015-09-13T11:00:00Z 2015-09-18T02:27:57Z This part is built by NJU_CHINA using GATEWAY technique. We standardized this part by ourselves. This is a engineered TAL effector that can bind with the DNA sequence 5'-GGAGGCACCGGTGG-3' false false _2214_ 21134 21134 9 false The part sequence should be able to correctly and efficiently bind with corresponding DNA, so we used bioinformatics tools to help us designing this sequence. false Xinyuan Qiu annotation2454113 1 DNA Binding domain range2454113 1 508 1872 annotation2454114 1 TALE1 range2454114 1 1 2087 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K1789016 1 BBa_K1789016 GFP_N2 2015-09-13T11:00:00Z 2015-09-14T08:35:17Z We obtained it through the contribution of our basic subparts. This device is a negative control of GFP with scaf. false false _2214_ 26299 26299 9 false This part is designed to be a negative control which is without the scaf, so it will prove that scaf can improve the fluorescence of GFP. false Dongyu Fan component2454391 1 BBa_K1789004 component2454384 1 BBa_K1789003 component2454398 1 BBa_B0015 component2454386 1 BBa_K1789002 component2454381 1 BBa_K1789008 component2454389 1 BBa_K1789009 component2454378 1 BBa_K1789012 annotation2454386 1 BBa_K1789002 range2454386 1 2805 2819 annotation2454384 1 BBa_K1789003 range2454384 1 2323 2796 annotation2454389 1 BBa_K1789009 range2454389 1 2826 4913 annotation2454398 1 BBa_B0015 range2454398 1 5179 5307 annotation2454378 1 BBa_K1789012 range2454378 1 1 223 annotation2454381 1 BBa_K1789008 range2454381 1 230 2316 annotation2454391 1 BBa_K1789004 range2454391 1 4922 5170 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K1789012 1 BBa_K1789012 P+R 2015-09-13T11:00:00Z 2015-09-14T07:22:47Z It is formed by our basic parts. This part is a promoter. It has a strong RBS restriction site following it which can guarantee the high efficiency of our project. false false _2214_ 26298 26298 9 false We want to construct a promoter which has RBS30 itself for RBS30 is small. If we supply a promoter with RBS, the efficiency of building will be high. false Jiaqi Sun component2454130 1 BBa_K1789002 component2454122 1 BBa_R0010 annotation2454122 1 BBa_R0010 range2454122 1 1 200 annotation2454130 1 BBa_K1789002 range2454130 1 209 223 BBa_K1789016_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatggctccaaagaagaagcgtaaggtagactacaaagaccatgacggtgattataaagatcatgacatcgattacaaggatgacgatgacaagggtaccgtggatctacgcacgctcggctacagccagcagcaacaggagaagatcaaaccgaaggttcgttcgacagtggcgcagcaccacgaggcactggtcggccacgggtttacacacgcgcacatcgttgcgctcagccaacacccggcagcgttagggaccgtcgctgtcacctatcagcatatcatcaccgcgttgccagaggcgacacacgaagatatcgttggcgtcggcaaacagtggtccggcgcacgcgctctggaggccttgctcacggatgcgggagagttgagaggtccaccgttacagttggacacaggccaacttgtgaagattgcaaaacgtggcggcgtgaccgcaatggaggcagtgcatgcaagccgcaatgcactgacgggtgcccccctgaacctgaccccagatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagaccgtccaacgccttctaccagttctctgtcaagcccacggactaaccccagcgcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagacagtccaacgccttctaccagttctctgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggcccttgagacagtccaacgccttcttccagttctttgtcaagctcacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacagcgcctcctccagttctttgtcaagctcacgggctcaccccagatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggcactcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggcggtcgaccggcgctggagagcattgttgcccagttatctcgccctgatccggcgttggccgcgttgaccaacgaccacctcgtcgccttggcctgcctcggcggacgtcctgcgatggatgcagtgaaaaagggattgccgcacgcgccggaattgatccgtagagtcaatcgccgtattggcgaacgcacatcccatcgcgttgccggatcctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaataatactagagattaaagaggagaaatactagatggctccaaagaagaagcgtaaggtagactacaaagaccatgacggtgattataaagatcatgacatcgattacaaggatgacgatgacaagggtaccgtggatctacgcacgctcggctacagccagcagcaacaggagaagatcaaaccgaaggttcgttcgacagtggcgcagcaccacgaggcactggtcggccacgggtttacacacgcgcacatcgttgcgctcagccaacacccggcagcgttagggaccgtcgctgtcacctatcagcatatcatcaccgcgttgccagaggcgacacacgaagatatcgttggcgtcggcaaacagtggtccggcgcacgcgctctggaggccttgctcacggatgcgggagagttgagaggtccaccgttacagttggacacaggccaacttgtgaagattgcaaaacgtggcggcgtgaccgcaatggaggcagtgcatgcaagccgcaatgcactgacgggtgcccccctgaacctgacccctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtaatgggggtggcaaacaggctcttgaaaccgtgcaacgactgctcccagttctctgtcaagcccacggcctcaccccggcgcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagaccgtccaacgccttctaccagttctctgtcaagcccacggactaaccccagcgcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagaccgtccaacgccttctaccagttctctgtcaagcccacggactaaccccagcgcaagttgtagcgattgctagtcatgacggtggcaaacaggcccttgagacagtccaacgccttcttccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggctcttgaaaccgtgcaacgactgcttccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggctcttgaaaccgtgcagcgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggcggtcgaccggcgctggagagcattgttgcccagttatctcgccctgatccggcgttggccgcgttgaccaacgaccacctcgtcgccttggcctgcctcggcggacgtcctgcgatggatgcagtgaaaaagggattgccgcacgcgccggaattgatccgtagagtcaatcgccgtattggcgaacgcacatcccatcgcgttgccggatcctactagagaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1789009_sequence 1 atggctccaaagaagaagcgtaaggtagactacaaagaccatgacggtgattataaagatcatgacatcgattacaaggatgacgatgacaagggtaccgtggatctacgcacgctcggctacagccagcagcaacaggagaagatcaaaccgaaggttcgttcgacagtggcgcagcaccacgaggcactggtcggccacgggtttacacacgcgcacatcgttgcgctcagccaacacccggcagcgttagggaccgtcgctgtcacctatcagcatatcatcaccgcgttgccagaggcgacacacgaagatatcgttggcgtcggcaaacagtggtccggcgcacgcgctctggaggccttgctcacggatgcgggagagttgagaggtccaccgttacagttggacacaggccaacttgtgaagattgcaaaacgtggcggcgtgaccgcaatggaggcagtgcatgcaagccgcaatgcactgacgggtgcccccctgaacctgacccctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtaatgggggtggcaaacaggctcttgaaaccgtgcaacgactgctcccagttctctgtcaagcccacggcctcaccccggcgcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagaccgtccaacgccttctaccagttctctgtcaagcccacggactaaccccagcgcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagaccgtccaacgccttctaccagttctctgtcaagcccacggactaaccccagcgcaagttgtagcgattgctagtcatgacggtggcaaacaggcccttgagacagtccaacgccttcttccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggctcttgaaaccgtgcaacgactgcttccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggctcttgaaaccgtgcagcgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggcggtcgaccggcgctggagagcattgttgcccagttatctcgccctgatccggcgttggccgcgttgaccaacgaccacctcgtcgccttggcctgcctcggcggacgtcctgcgatggatgcagtgaaaaagggattgccgcacgcgccggaattgatccgtagagtcaatcgccgtattggcgaacgcacatcccatcgcgttgccggatcc BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K1789004_sequence 1 aagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K1789002_sequence 1 attaaagaggagaaa BBa_K1789008_sequence 1 atggctccaaagaagaagcgtaaggtagactacaaagaccatgacggtgattataaagatcatgacatcgattacaaggatgacgatgacaagggtaccgtggatctacgcacgctcggctacagccagcagcaacaggagaagatcaaaccgaaggttcgttcgacagtggcgcagcaccacgaggcactggtcggccacgggtttacacacgcgcacatcgttgcgctcagccaacacccggcagcgttagggaccgtcgctgtcacctatcagcatatcatcaccgcgttgccagaggcgacacacgaagatatcgttggcgtcggcaaacagtggtccggcgcacgcgctctggaggccttgctcacggatgcgggagagttgagaggtccaccgttacagttggacacaggccaacttgtgaagattgcaaaacgtggcggcgtgaccgcaatggaggcagtgcatgcaagccgcaatgcactgacgggtgcccccctgaacctgaccccagatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagaccgtccaacgccttctaccagttctctgtcaagcccacggactaaccccagcgcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagacagtccaacgccttctaccagttctctgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggcccttgagacagtccaacgccttcttccagttctttgtcaagctcacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacagcgcctcctccagttctttgtcaagctcacgggctcaccccagatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggcactcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggcggtcgaccggcgctggagagcattgttgcccagttatctcgccctgatccggcgttggccgcgttgaccaacgaccacctcgtcgccttggcctgcctcggcggacgtcctgcgatggatgcagtgaaaaagggattgccgcacgcgccggaattgatccgtagagtcaatcgccgtattggcgaacgcacatcccatcgcgttgccggatcc BBa_K1789012_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1789003_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaataa BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z