We obtained it through the contribution of our basic subparts.
This device is a positive control of GFP with scaf.
This part is designed to be a positive control which contains the complete GFP protein.
Double terminator consisting of BBa_B0010 and BBa_B0012
This part is a promoter. It has a strong RBS restriction site following it which can guarantee the high efficiency of our project.
We want to construct a promoter which has RBS30 itself for RBS30 is small. If we supply a promoter with RBS, the efficiency of building will be high.
Amplified by PCR from <partinfo>BBa_J54103</partinfo>
This is GFP from Biobrick <partinfo>BBa_J54103</partinfo>, which we have sequenced and re-cloned to RFC[10] standard in pSB1C3.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
Transcriptional terminator consisting of a 64 bp stem-loop.
The part sequence was gained from the registry, the part is annealed from the ssDNA synthesized by Life(R).
This part is another copy of the RBS30 (BBa_B0030). It is a Strong RBS based on Ron Weiss thesis.
The reason why we built this part by ourselves is that the copy of B0030 in the 2015 DNA distribution we received is sequenced as incorrect. And same problem also occurred in several other teams.
Conversion of the iGEM parts registry to SBOL2.1