LOCUS BBa_K1789019 5594 bp DNA linear 25-Nov-2024 DEFINITION GFP_S2 ACCESSION BBa_K1789019 VERSION BBa_K1789019.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..223 /label=RBS30 RBS 209..223 /label=BBa_K1789002 misc_feature 1..223 /label=BBa_K1789012 misc_feature 737..2101 /label=DNA Binding domain CDS 230..2316 /label=TALE1 CDS 230..2316 /label=BBa_K1789008 CDS 2323..2793 /label=GFP1 misc_feature 2794..2796 /label=termination codon CDS 2323..2796 /label=BBa_K1789003 RBS 2805..2819 /label=RBS30 RBS 2805..2819 /label=BBa_K1789002 CDS 2826..4913 /label=TALE3 misc_feature 3333..4697 /label=DNA Binding Domain CDS 2826..4913 /label=BBa_K1789010 CDS 4922..5170 /label=GFP2 CDS 4922..5170 /label=BBa_K1789004 stem_loop 5190..5233 /label=stem_loop misc_feature 5179..5258 /label=BBa_B0010 terminator 5179..5258 /label=BBa_B0010 stem_loop 5274..5293 /label=T7 TE misc_feature 5300..5300 /label=stop polyA_site 5294..5307 /label=polya misc_feature 5267..5307 /label=BBa_B0012 terminator 5267..5307 /label=BBa_B0012 terminator 5179..5307 /label=BBa_B0015 misc_feature 5316..5777 /label=SCAF2 misc_feature 5316..5329 /label=BM1 misc_feature 5336..5349 /label=BM2 misc_feature 5316..5594 /label=BBa_K1789006 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tggctccaaa 241 gaagaagcgt aaggtagact acaaagacca tgacggtgat tataaagatc atgacatcga 301 ttacaaggat gacgatgaca agggtaccgt ggatctacgc acgctcggct acagccagca 361 gcaacaggag aagatcaaac cgaaggttcg ttcgacagtg gcgcagcacc acgaggcact 421 ggtcggccac gggtttacac acgcgcacat cgttgcgctc agccaacacc cggcagcgtt 481 agggaccgtc gctgtcacct atcagcatat catcaccgcg ttgccagagg cgacacacga 541 agatatcgtt ggcgtcggca aacagtggtc cggcgcacgc gctctggagg ccttgctcac 601 ggatgcggga gagttgagag gtccaccgtt acagttggac acaggccaac ttgtgaagat 661 tgcaaaacgt ggcggcgtga ccgcaatgga ggcagtgcat gcaagccgca atgcactgac 721 gggtgccccc ctgaacctga ccccagatca agttgtagcg attgctagta acaatggtgg 781 caaacaggct ctcgaaaccg tacaacgact cctcccagtt ctctgtcaag cccacggact 841 aactcctgat caagttgtag cgattgctag taacaatggt ggcaaacagg ctctcgaaac 901 cgtacaacga ctcctcccag ttctctgtca agcccacgga ctaactcctg atcaagttgt 961 agcgattgct agtaatattg gtggcaaaca ggcacttgag acggttcagc gcctccttcc 1021 agttctttgt caagctcacg gactaactcc tgatcaagtt gtagcgattg ctagtaacaa 1081 tggtggcaaa caggctctcg aaaccgtaca acgactcctc ccagttctct gtcaagccca 1141 cggactaact cctgatcaag ttgtagcgat tgctagtaac aatggtggca aacaggctct 1201 cgaaaccgta caacgactcc tcccagttct ctgtcaagct cacggactca ccccagatca 1261 agttgtagcg attgctagtc atgacggtgg caaacaggct cttgagaccg tccaacgcct 1321 tctaccagtt ctctgtcaag cccacggact aaccccagcg caagttgtag cgattgctag 1381 taatattggt ggcaaacagg cacttgagac ggttcagcgc ctccttccag ttctttgtca 1441 agctcacgga ctcaccccag atcaagttgt agcgattgct agtcatgacg gtggcaaaca 1501 ggctcttgag acagtccaac gccttctacc agttctctgt caagctcacg gactcacccc 1561 agatcaagtt gtagcgattg ctagtcatga cggtggcaaa caggcccttg agacagtcca 1621 acgccttctt ccagttcttt gtcaagctca cggactaact cctgatcaag ttgtagcgat 1681 tgctagtaac aatggtggca aacaggctct cgaaaccgta cagcgcctcc tccagttctt 1741 tgtcaagctc acgggctcac cccagatcaa gttgtagcga ttgctagtaa caatggtggc 1801 aaacaggctc tcgaaaccgt acaacgactc ctcccagttc tctgtcaagc ccacggacta 1861 actcctgatc aagttgtagc gattgctagt aatgggggtg gcaaacaggc actcgaaacc 1921 gtacaacgac tcctcccagt tctctgtcaa gcccacggac taactcctga tcaagttgta 1981 gcgattgcta gtaacaatgg tggcaaacag gctctcgaaa ccgtacaacg actcctccca 2041 gttctctgtc aagcccacgg actaactcct gatcaagttg tagcgattgc tagtaacaat 2101 ggcggtcgac cggcgctgga gagcattgtt gcccagttat ctcgccctga tccggcgttg 2161 gccgcgttga ccaacgacca cctcgtcgcc ttggcctgcc tcggcggacg tcctgcgatg 2221 gatgcagtga aaaagggatt gccgcacgcg ccggaattga tccgtagagt caatcgccgt 2281 attggcgaac gcacatccca tcgcgttgcc ggatcctact agatgcgtaa aggagaagaa 2341 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa 2401 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt 2461 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat 2521 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt 2581 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac 2641 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa 2701 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac 2761 tcacacaatg tatacatcat ggcagacaaa caataatact agagattaaa gaggagaaat 2821 actagatggc tccaaagaag aagcgtaagg tagactacaa agaccatgac ggtgattata 2881 aagatcatga catcgattac aaggatgacg atgacaaggg taccgtggat ctacgcacgc 2941 tcggctacag ccagcagcaa caggagaaga tcaaaccgaa ggttcgttcg acagtggcgc 3001 agcaccacga ggcactggtc ggccacgggt ttacacacgc gcacatcgtt gcgctcagcc 3061 aacacccggc agcgttaggg accgtcgctg tcacctatca gcatatcatc accgcgttgc 3121 cagaggcgac acacgaagat atcgttggcg tcggcaaaca gtggtccggc gcacgcgctc 3181 tggaggcctt gctcacggat gcgggagagt tgagaggtcc accgttacag ttggacacag 3241 gccaacttgt gaagattgca aaacgtggcg gcgtgaccgc aatggaggca gtgcatgcaa 3301 gccgcaatgc actgacgggt gcccccctga acctgacccc agatcaagtt gtagcgattg 3361 ctagtcatga cggtggcaaa caggctcttg agaccgtcca acgccttcta ccagttctct 3421 gtcaagccca cggactaacc ccagcgcaag ttgtagcgat tgctagtaat gggggtggca 3481 aacaggctct tgaaaccgtg caacgactgc tcccagttct ctgtcaagcc cacggcctca 3541 ccccggcgca agttgtagcg attgctagta atgggggtgg caaacaggct cttgaaaccg 3601 tgcaacgact gctcccagtt ctctgtcaag cccacggcct caccccggcg caagttgtag 3661 cgattgctag taatgggggt ggcaaacagg ctcttgaaac cgtgcaacga ctgctcccag 3721 ttctctgtca agcccacggc ctcaccccgg cgcaagttgt agcgattgct agtcatgacg 3781 gtggcaaaca ggctcttgag accgtccaac gccttctacc agttctctgt caagcccacg 3841 gactaacccc agcgcaagtt gtagcgattg ctagtcatga cggtggcaaa caggcccttg 3901 agacagtcca acgccttcta ccagttctct gccaagcaca cggactaact cctgatcaag 3961 ttgtagcgat tgctagtaat attggtggca aacaggcact tgagacggtt cagcgcctcc 4021 ttccagttct ttgtcaagct cacggactca ccccagatca agttgtagcg attgctagtc 4081 atgacggtgg caaacaggct cttgagaccg tccaacgcct tctaccagtt ctctgtcaag 4141 ctcacggact caccccagat caagttgtag cgattgctag taatattggt ggcaaacagg 4201 cacttgagac ggttcagcgc ctccttccag ttctttgtca agctcacgga ctcaccccag 4261 atcaagttgt agcgattgct agtaatattg gtggcaaaca ggcacttgag acggttcagc 4321 gcctccttcc agttctctgt caagcccacg gactaactcc tgatcaagtt gtagcgattg 4381 ctagtaatat tggtggcaaa caggcacttg agacggttca gcgcctcctt ccagttctct 4441 gtcaagccca cggactaact cctgatcaag ttgtagcgat tgctagtaat gggggtggca 4501 aacaggctct tgaaaccgtg cagcgactcc tcccagttct ctgtcaagcc cacggactaa 4561 ctcctgatca agttgtagcg attgctagta atattggtgg caaacaggca cttgagacgg 4621 ttcagcgcct ccttccagtt ctttgtcaag ctcacggact caccccagat caagttgtag 4681 cgattgctag taacaatggc ggtcgaccgg cgctggagag cattgttgcc cagttatctc 4741 gccctgatcc ggcgttggcc gcgttgacca acgaccacct cgtcgccttg gcctgcctcg 4801 gcggacgtcc tgcgatggat gcagtgaaaa agggattgcc gcacgcgccg gaattgatcc 4861 gtagagtcaa tcgccgtatt ggcgaacgca catcccatcg cgttgccgga tcctactaga 4921 gaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca 4981 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga 5041 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca 5101 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata 5161 caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 5221 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 5281 cttcgggtgg gcctttctgc gtttatatac tagagctaga tggaggcacc ggtggtgata 5341 aacacctttc tggaggcacc ggtggtgata aacacctttc tggaggcacc ggtggtgata 5401 aacacctttc tggaggcacc ggtggtgata aacacctttc tggaggcacc ggtggtgata 5461 aacacctttc tggaggcacc ggtggtgata aacacctttc tggaggcacc ggtggtgata 5521 aacacctttc tggaggcacc ggtggtgata aacacctttc tggaggcacc ggtggtgata 5581 aacacctttc tgga //