LOCUS BBa_K1789023 8245 bp DNA linear 31-Oct-2024 DEFINITION IAA(+/+)S3 ACCESSION BBa_K1789023 VERSION BBa_K1789023.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..223 /label=RBS30 RBS 209..223 /label=BBa_K1789002 misc_feature 1..223 /label=BBa_K1789012 misc_feature 737..2101 /label=DNA Binding domain CDS 230..2316 /label=TALE1 CDS 230..2316 /label=BBa_K1789008 CDS 2323..3960 /label=IaaM CDS 2323..3960 /label=BBa_K1789000 RBS 3969..3983 /label=RBS30 RBS 3969..3983 /label=BBa_K1789002 misc_feature 4503..5861 /label=DNA Binding Domain CDS 3990..6077 /label=TALE2 CDS 3990..6077 /label=BBa_K1789009 CDS 6084..7418 /label=IaaH CDS 6084..7418 /label=BBa_K1789001 stem_loop 7438..7481 /label=stem_loop misc_feature 7427..7506 /label=BBa_B0010 terminator 7427..7506 /label=BBa_B0010 stem_loop 7522..7541 /label=T7 TE misc_feature 7548..7548 /label=stop polyA_site 7542..7555 /label=polya misc_feature 7515..7555 /label=BBa_B0012 terminator 7515..7555 /label=BBa_B0012 terminator 7427..7555 /label=BBa_B0015 misc_feature 7564..7577 /label=TALE1 BINDING SITE misc_feature 7595..7609 /label=TALE2 BINDING SITE misc_feature 7564..8245 /label=SCAF3 misc_feature 7564..8245 /label=BBa_K1789007 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tggctccaaa 241 gaagaagcgt aaggtagact acaaagacca tgacggtgat tataaagatc atgacatcga 301 ttacaaggat gacgatgaca agggtaccgt ggatctacgc acgctcggct acagccagca 361 gcaacaggag aagatcaaac cgaaggttcg ttcgacagtg gcgcagcacc acgaggcact 421 ggtcggccac gggtttacac acgcgcacat cgttgcgctc agccaacacc cggcagcgtt 481 agggaccgtc gctgtcacct atcagcatat catcaccgcg ttgccagagg cgacacacga 541 agatatcgtt ggcgtcggca aacagtggtc cggcgcacgc gctctggagg ccttgctcac 601 ggatgcggga gagttgagag gtccaccgtt acagttggac acaggccaac ttgtgaagat 661 tgcaaaacgt ggcggcgtga ccgcaatgga ggcagtgcat gcaagccgca atgcactgac 721 gggtgccccc ctgaacctga ccccagatca agttgtagcg attgctagta acaatggtgg 781 caaacaggct ctcgaaaccg tacaacgact cctcccagtt ctctgtcaag cccacggact 841 aactcctgat caagttgtag cgattgctag taacaatggt ggcaaacagg ctctcgaaac 901 cgtacaacga ctcctcccag ttctctgtca agcccacgga ctaactcctg atcaagttgt 961 agcgattgct agtaatattg gtggcaaaca ggcacttgag acggttcagc gcctccttcc 1021 agttctttgt caagctcacg gactaactcc tgatcaagtt gtagcgattg ctagtaacaa 1081 tggtggcaaa caggctctcg aaaccgtaca acgactcctc ccagttctct gtcaagccca 1141 cggactaact cctgatcaag ttgtagcgat tgctagtaac aatggtggca aacaggctct 1201 cgaaaccgta caacgactcc tcccagttct ctgtcaagct cacggactca ccccagatca 1261 agttgtagcg attgctagtc atgacggtgg caaacaggct cttgagaccg tccaacgcct 1321 tctaccagtt ctctgtcaag cccacggact aaccccagcg caagttgtag cgattgctag 1381 taatattggt ggcaaacagg cacttgagac ggttcagcgc ctccttccag ttctttgtca 1441 agctcacgga ctcaccccag atcaagttgt agcgattgct agtcatgacg gtggcaaaca 1501 ggctcttgag acagtccaac gccttctacc agttctctgt caagctcacg gactcacccc 1561 agatcaagtt gtagcgattg ctagtcatga cggtggcaaa caggcccttg agacagtcca 1621 acgccttctt ccagttcttt gtcaagctca cggactaact cctgatcaag ttgtagcgat 1681 tgctagtaac aatggtggca aacaggctct cgaaaccgta cagcgcctcc tccagttctt 1741 tgtcaagctc acgggctcac cccagatcaa gttgtagcga ttgctagtaa caatggtggc 1801 aaacaggctc tcgaaaccgt acaacgactc ctcccagttc tctgtcaagc ccacggacta 1861 actcctgatc aagttgtagc gattgctagt aatgggggtg gcaaacaggc actcgaaacc 1921 gtacaacgac tcctcccagt tctctgtcaa gcccacggac taactcctga tcaagttgta 1981 gcgattgcta gtaacaatgg tggcaaacag gctctcgaaa ccgtacaacg actcctccca 2041 gttctctgtc aagcccacgg actaactcct gatcaagttg tagcgattgc tagtaacaat 2101 ggcggtcgac cggcgctgga gagcattgtt gcccagttat ctcgccctga tccggcgttg 2161 gccgcgttga ccaacgacca cctcgtcgcc ttggcctgcc tcggcggacg tcctgcgatg 2221 gatgcagtga aaaagggatt gccgcacgcg ccggaattga tccgtagagt caatcgccgt 2281 attggcgaac gcacatccca tcgcgttgcc ggatcctact agatgtttgg accggatttc 2341 ccgtttgcat ttgatgattg gatcgaacat cctaaaggac ttgggtcaat tccggcagaa 2401 aatcacggcg ctgaggttgc aattatcggg gccggcattg caggcttagt agcagcatat 2461 gaattaatga agatgggtct gaaacctgta gtctatgagg cctccaaaat gggaggtcgc 2521 ttacggtcac aggaatttga aggagcgaag ggaattgttg cggaactggg cggtatgcgc 2581 tttccagtct cctccacagc gttctttcat tacgttgata aactgggcct tgaaagtcgt 2641 ccgttcccta accctttgac ggcggccagt ggcagcacgg tgattgacct ggaaggtacc 2701 acgtactatg cacagatgtt atcagatctc ccagccctgt tccaagaagt ggcggatgca 2761 tgggcagacg ccttagaatc aggctctcaa tttggagata ttcaacaggc cattcgtgat 2821 tctgacgtac cgcgtctcaa ggaactgtgg aacaaactcg tgcctttgtg ggacgatcgc 2881 actttctatg actttgtagc gacctcaaag gcctttgcga aattaagctt ttaccatcgt 2941 gaggttttcg gacaagttgg ttttggtaca ggggggtggg attctgattt tccaaatagt 3001 atgttagaaa tctttcgtgt tgtgatgacg aattgtgatg agcaccagca tctgattgtt 3061 ggtggcgtac agcaagtgcc tgtaggcctg tggtcgcacg tgcctgaacg ctgcgcacat 3121 tggccaaagg gtacgtctct ttcctcactt catcggggtg ccccgcgccc aggtgtcaaa 3181 cgtattgcac gtgctgaaga tggttcattt acggttacag ataattgggg cgatacacgt 3241 caatacgctg ccgttttgac aacctgtcaa tcgtggctgc ttacaacgca gattgaatgt 3301 gaggaatctt tgttttcaca gaagatgtgg atggcgctgg atcgcacgcg ttacatgcag 3361 tcttctaaaa cttttgtgat ggttgatcgg ccgttttgga aggacaaaga tccggaaacg 3421 ggtcgcgatc ttatgtccat gaccctcacc gatcgcttaa cgcgcggcac atacttattt 3481 gataatggcg acgacaagcc aggcgttatt tgcctgagtt actcatggat gagtgacgct 3541 ttaaaaatgt taccgcaacc gatcgagaaa cgcgtcaaat tagctttaga tgcccttaaa 3601 aagatttacc cgaaagtgga tatcgccgca cgtattattg gtgacccgat tactgtgagt 3661 tgggaagcgg atccgcattt cctgggggca tttaaaggcg cgttaccggg ccattaccgt 3721 tacaaccagc gcatgtacgc gcattttatg cagcaggaca tgccgtcaga acagcgtggc 3781 atgtttattg ccggtgatga tgtatcttgg acaccggcat gggttgaggg agcagtacaa 3841 acgtcattga acgcggtgtg gggcatcatg aaccatttcg gagggaaaac ccacgcagag 3901 aatccgggtc ctggcgacgt tttccatgag attggaccga tcgcgctggg ggactaataa 3961 tactagagat taaagaggag aaatactaga tggctccaaa gaagaagcgt aaggtagact 4021 acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca 4081 agggtaccgt ggatctacgc acgctcggct acagccagca gcaacaggag aagatcaaac 4141 cgaaggttcg ttcgacagtg gcgcagcacc acgaggcact ggtcggccac gggtttacac 4201 acgcgcacat cgttgcgctc agccaacacc cggcagcgtt agggaccgtc gctgtcacct 4261 atcagcatat catcaccgcg ttgccagagg cgacacacga agatatcgtt ggcgtcggca 4321 aacagtggtc cggcgcacgc gctctggagg ccttgctcac ggatgcggga gagttgagag 4381 gtccaccgtt acagttggac acaggccaac ttgtgaagat tgcaaaacgt ggcggcgtga 4441 ccgcaatgga ggcagtgcat gcaagccgca atgcactgac gggtgccccc ctgaacctga 4501 cccctgatca agttgtagcg attgctagta acaatggtgg caaacaggct ctcgaaaccg 4561 tacaacgact cctcccagtt ctctgtcaag cccacggact aactcctgat caagttgtag 4621 cgattgctag taatattggt ggcaaacagg cacttgagac ggttcagcgc ctccttccag 4681 ttctttgtca agctcacgga ctcaccccag atcaagttgt agcgattgct agtaatgggg 4741 gtggcaaaca ggctcttgaa accgtgcaac gactgctccc agttctctgt caagcccacg 4801 gcctcacccc ggcgcaagtt gtagcgattg ctagtaatat tggtggcaaa caggcacttg 4861 agacggttca gcgcctcctt ccagttcttt gtcaagctca cggactcacc ccagatcaag 4921 ttgtagcgat tgctagtaat attggtggca aacaggcact tgagacggtt cagcgcctcc 4981 ttccagttct ttgtcaagct cacggactca ccccagatca agttgtagcg attgctagta 5041 atattggtgg caaacaggca cttgagacgg ttcagcgcct ccttccagtt ctttgtcaag 5101 ctcacggact caccccagat caagttgtag cgattgctag tcatgacggt ggcaaacagg 5161 ctcttgagac cgtccaacgc cttctaccag ttctctgtca agcccacgga ctaaccccag 5221 cgcaagttgt agcgattgct agtaatattg gtggcaaaca ggcacttgag acggttcagc 5281 gcctccttcc agttctttgt caagctcacg gactcacccc agatcaagtt gtagcgattg 5341 ctagtcatga cggtggcaaa caggctcttg agaccgtcca acgccttcta ccagttctct 5401 gtcaagccca cggactaacc ccagcgcaag ttgtagcgat tgctagtcat gacggtggca 5461 aacaggccct tgagacagtc caacgccttc ttccagttct ctgtcaagcc cacggactaa 5521 ctcctgatca agttgtagcg attgctagta atgggggtgg caaacaggct cttgaaaccg 5581 tgcaacgact gcttccagtt ctctgtcaag cccacggact aactcctgat caagttgtag 5641 cgattgctag taatgggggt ggcaaacagg ctcttgaaac cgtgcagcga ctcctcccag 5701 ttctctgtca agcccacgga ctaactcctg atcaagttgt agcgattgct agtaatgggg 5761 gtggcaaaca ggcacttgag acggttcagc gcctccttcc agttctttgt caagctcacg 5821 gactcacccc agatcaagtt gtagcgattg ctagtcatga cggcggtcga ccggcgctgg 5881 agagcattgt tgcccagtta tctcgccctg atccggcgtt ggccgcgttg accaacgacc 5941 acctcgtcgc cttggcctgc ctcggcggac gtcctgcgat ggatgcagtg aaaaagggat 6001 tgccgcacgc gccggaattg atccgtagag tcaatcgccg tattggcgaa cgcacatccc 6061 atcgcgttgc cggatcctac tagatgatta cgcttgaatc tctgtgccgc gcattggcag 6121 acgaacagat tgccgcagaa gaattgcgcg agcgcgcgct ggatacagag gctcgcttaa 6181 cactgttaaa ttgcttcatt cgcgaaggcg acgccgtaag ccaattcggt gaagcggatc 6241 aggcgcgtaa aggaacttca ctttgggggg tgcctgtgag ttttaaggat aacatctgtg 6301 ttcgcgggct gccactgacg gcgggtaccc gcggcatgag cggctttatt gccgatcagg 6361 atgcagcaat cgtgagccag ttaaaagcat tgggagctgt ggtcgcgggc aaaaataaca 6421 tgcatgagct gtcatttggc gtcacgagca tcaatccaca ttggggcgcg gttggaaacc 6481 cggtggctcc aggatattgc gccggaggtt ctagcggagg ttccgctgcg gcggtcgcat 6541 ctggaattgt cccgctcagc gtcgggacgg acacaggcgg ctcaatccgt attccggcgg 6601 cattttgcgg cattacgggt tttcgcccta ctacaggtcg cttgagtacg gcggggatca 6661 ttccggtcag ccacacgaag gattgtgtcg gcctcctgac gcgcacagct ggcgacgctg 6721 aatttgtgta tgggctgctg tcaggaaaac agcagagttt ccctcttaat cgcacgggac 6781 cttgccgcat cggcctgccg gtgagcatgt ggtctgactt agatggcgaa gtagaacgtg 6841 cctgtatcaa tgcgctgagc ctcttacgca aaacgggctt tgaatttgtt gagatcgacg 6901 atgcggacat tgtggaattg aaccagacat taaccttcac ggtgccgctg tacgaatttt 6961 tcgcggattt tgctcaatcc ttgttgtcct taggttggaa acatggcatc catcatattt 7021 ttgcacaagt ggatgatgca aatgtgaaag gcattattaa tcaccatctg ggagagggcg 7081 cgattaagcc ggcacattat ctgagttctc tccagaatgg agagctgctg aaacgcaaga 7141 tggatgagct ttttgcgcgc catcacatta aacttttagg gtatccgacg gtcccgtgtc 7201 gcgtgcctca tctggaccat gcagatcgtc cggaattttt tagccaggcc atccgtaaca 7261 cagacctcgc aagcaacgcg atgttaccaa gcattacaat tccggtcggt ccggaaggcc 7321 gcctgccggt cggccttagt tttgatgcgc ctcgtgctcg tgatgcgttt ctcttaagca 7381 atgttagttt gatcgaaaaa gtgttaaaag gctaataata ctagagccag gcatcaaata 7441 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 7501 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 7561 gagtggaggc accggtggac agtagctaca accctgataa acacctttca cagtagctac 7621 aaccctggag gcaccggtgg acagtagcta caaccctgat aaacaccttt cacagtagct 7681 acaaccctgg aggcaccggt ggacagtagc tacaaccctg ataaacacct ttcacagtag 7741 ctacaaccct ggaggcaccg gtggacagta gctacaaccc tgataaacac ctttcacagt 7801 agctacaacc ctggaggcac cggtggacag tagctacaac cctgataaac acctttcaca 7861 gtagctacaa ccctggaggc accggtggac agtagctaca accctgataa acacctttca 7921 cagtagctac aaccctggag gcaccggtgg acagtagcta caaccctgat aaacaccttt 7981 cacagtagct acaaccctgg aggcaccggt ggacagtagc tacaaccctg ataaacacct 8041 ttcacagtag ctacaaccct ggaggcaccg gtggacagta gctacaaccc tgataaacac 8101 ctttcacagt agctacaacc ctggaggcac cggtggacag tagctacaac cctgataaac 8161 acctttcaca gtagctacaa ccctggaggc accggtggac agtagctaca accctgataa 8221 acacctttca cagtagctac aaccc //