BBa_K1789009 1 BBa_K1789009 TALE2 2015-09-13T11:00:00Z 2015-09-14T07:20:22Z This part is built and standardized by ourselves using GATEWAY technique. This is a engineered TAL effector that can bind with the DNA sequence 5'-GATAAACACCTTTC-3' false false _2214_ 21134 21134 9 false The part sequence should be able to correctly and efficiently bind with corresponding DNA, so we used bioinformatics tools to help us designing this sequence. false Xinyuan Qiu annotation2454121 1 TALE2 range2454121 1 1 2088 annotation2454120 1 DNA Binding Domain range2454120 1 514 1872 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961227 1 start range1961227 1 173 173 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961224 1 -35 range1961224 1 137 142 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 BBa_K1789023 1 BBa_K1789023 IAA(+/+)S3 2015-09-13T11:00:00Z 2015-09-14T07:35:36Z We obtained it through the contribution of our basic subparts. This is a device which contains the sequence of TALE based IAAM, TALE based IAAH and scaffold 3. Through this device, we can test and verify that our scaffold can strengthen the function of IAA. false false _2214_ 26296 26296 9 false This is the experimental group of our project. Our theory is feasible if the functional parameter of this group is stronger than the group of negative control. false Chushu Zhu component2454195 1 BBa_B0015 component2454198 1 BBa_K1789007 component2454183 1 BBa_K1789002 component2454179 1 BBa_K1789008 component2454188 1 BBa_K1789001 component2454186 1 BBa_K1789009 component2454176 1 BBa_K1789012 component2454181 1 BBa_K1789000 annotation2454188 1 BBa_K1789001 range2454188 1 6084 7418 annotation2454198 1 BBa_K1789007 range2454198 1 7564 8245 annotation2454181 1 BBa_K1789000 range2454181 1 2323 3960 annotation2454186 1 BBa_K1789009 range2454186 1 3990 6077 annotation2454179 1 BBa_K1789008 range2454179 1 230 2316 annotation2454195 1 BBa_B0015 range2454195 1 7427 7555 annotation2454176 1 BBa_K1789012 range2454176 1 1 223 annotation2454183 1 BBa_K1789002 range2454183 1 3969 3983 BBa_K1789007 1 BBa_K1789007 SCAF3 2015-09-13T11:00:00Z 2015-09-18T04:48:41Z This part is synthesized by company This part is a scaffold system contains the recognizing motifs of TALE1 and TALE2 false false _2214_ 21134 21134 9 false We designed this sequence to provide sites for our engineered TALE proteins to bind false Xinyuan Qiu annotation2454075 1 TALE1 BINDING SITE range2454075 1 1 14 annotation2470998 1 SCAF3 range2470998 1 1 682 annotation2454077 1 TALE2 BINDING SITE range2454077 1 32 46 BBa_K1789002 1 BBa_K1789002 RBS30 2015-09-13T11:00:00Z 2015-09-14T06:03:14Z The part sequence was gained from the registry, the part is annealed from the ssDNA synthesized by Life(R). This part is another copy of the RBS30 (BBa_B0030). It is a Strong RBS based on Ron Weiss thesis. false false _2214_ 21134 21134 9 false The reason why we built this part by ourselves is that the copy of B0030 in the 2015 DNA distribution we received is sequenced as incorrect. And same problem also occurred in several other teams. false Xinyuan Qiu annotation2454064 1 RBS30 range2454064 1 1 15 BBa_K1789008 1 BBa_K1789008 TALE1 2015-09-13T11:00:00Z 2015-09-18T02:27:57Z This part is built by NJU_CHINA using GATEWAY technique. We standardized this part by ourselves. This is a engineered TAL effector that can bind with the DNA sequence 5'-GGAGGCACCGGTGG-3' false false _2214_ 21134 21134 9 false The part sequence should be able to correctly and efficiently bind with corresponding DNA, so we used bioinformatics tools to help us designing this sequence. false Xinyuan Qiu annotation2454114 1 TALE1 range2454114 1 1 2087 annotation2454113 1 DNA Binding domain range2454113 1 508 1872 BBa_K1789001 1 BBa_K1789001 IaaH 2015-09-13T11:00:00Z 2015-09-14T05:49:28Z This part is gained from the part BBa_K515100 by PCR. This part is the coding sequence of the enzyme Indoleacetimide hydrolase Iaa H. This enzyme catalyses the hydrolysis of indoleacetamide to indoleacetate and ammonia. This part is gathered from the 2-step pathway in Pseudomonas savastanoi producing the auxin, indole-3-acetic acid. false false _2214_ 21134 21134 9 false In order to get the whole sequence of IAAH, we removed the RBS and other irrelevant sequences in K515100 when designing the PCR primers. false Xinyuan Qiu annotation2454035 1 IaaH range2454035 1 1 1335 BBa_K1789012 1 BBa_K1789012 P+R 2015-09-13T11:00:00Z 2015-09-14T07:22:47Z It is formed by our basic parts. This part is a promoter. It has a strong RBS restriction site following it which can guarantee the high efficiency of our project. false false _2214_ 26298 26298 9 false We want to construct a promoter which has RBS30 itself for RBS30 is small. If we supply a promoter with RBS, the efficiency of building will be high. false Jiaqi Sun component2454130 1 BBa_K1789002 component2454122 1 BBa_R0010 annotation2454130 1 BBa_K1789002 range2454130 1 209 223 annotation2454122 1 BBa_R0010 range2454122 1 1 200 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K1789000 1 BBa_K1789000 IaaM 2015-09-11T11:00:00Z 2015-09-12T02:27:07Z This part is gained from BBa_K515100 by PCR. The IAA producing pathway originates from Pseudomonas savastanoi. This part is the coding sequence of tryptophan-2-mono-oxygenase. This enzyme catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which acts as an intermediate to the production of indole-3-acetic acid in the IAM pathway. false false _2214_ 21134 21134 9 false The reason why er designed this part is that we needed IAA producing pathway as a reporter in our system, and the IAAM and IAAH CDS needed to be fused with the coding sequence of the TAL effector we engineered. So we used the part BBa_K515100, which contains the CDS of IaaM and IaaH, and used PCR to eliminate the RBS sequence from the 5' region of the CDS. false Xinyuan Qiu annotation2452420 1 IaaM range2452420 1 1 1638 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1789001_sequence 1 atgattacgcttgaatctctgtgccgcgcattggcagacgaacagattgccgcagaagaattgcgcgagcgcgcgctggatacagaggctcgcttaacactgttaaattgcttcattcgcgaaggcgacgccgtaagccaattcggtgaagcggatcaggcgcgtaaaggaacttcactttggggggtgcctgtgagttttaaggataacatctgtgttcgcgggctgccactgacggcgggtacccgcggcatgagcggctttattgccgatcaggatgcagcaatcgtgagccagttaaaagcattgggagctgtggtcgcgggcaaaaataacatgcatgagctgtcatttggcgtcacgagcatcaatccacattggggcgcggttggaaacccggtggctccaggatattgcgccggaggttctagcggaggttccgctgcggcggtcgcatctggaattgtcccgctcagcgtcgggacggacacaggcggctcaatccgtattccggcggcattttgcggcattacgggttttcgccctactacaggtcgcttgagtacggcggggatcattccggtcagccacacgaaggattgtgtcggcctcctgacgcgcacagctggcgacgctgaatttgtgtatgggctgctgtcaggaaaacagcagagtttccctcttaatcgcacgggaccttgccgcatcggcctgccggtgagcatgtggtctgacttagatggcgaagtagaacgtgcctgtatcaatgcgctgagcctcttacgcaaaacgggctttgaatttgttgagatcgacgatgcggacattgtggaattgaaccagacattaaccttcacggtgccgctgtacgaatttttcgcggattttgctcaatccttgttgtccttaggttggaaacatggcatccatcatatttttgcacaagtggatgatgcaaatgtgaaaggcattattaatcaccatctgggagagggcgcgattaagccggcacattatctgagttctctccagaatggagagctgctgaaacgcaagatggatgagctttttgcgcgccatcacattaaacttttagggtatccgacggtcccgtgtcgcgtgcctcatctggaccatgcagatcgtccggaattttttagccaggccatccgtaacacagacctcgcaagcaacgcgatgttaccaagcattacaattccggtcggtccggaaggccgcctgccggtcggccttagttttgatgcgcctcgtgctcgtgatgcgtttctcttaagcaatgttagtttgatcgaaaaagtgttaaaaggctaataa BBa_K1789009_sequence 1 atggctccaaagaagaagcgtaaggtagactacaaagaccatgacggtgattataaagatcatgacatcgattacaaggatgacgatgacaagggtaccgtggatctacgcacgctcggctacagccagcagcaacaggagaagatcaaaccgaaggttcgttcgacagtggcgcagcaccacgaggcactggtcggccacgggtttacacacgcgcacatcgttgcgctcagccaacacccggcagcgttagggaccgtcgctgtcacctatcagcatatcatcaccgcgttgccagaggcgacacacgaagatatcgttggcgtcggcaaacagtggtccggcgcacgcgctctggaggccttgctcacggatgcgggagagttgagaggtccaccgttacagttggacacaggccaacttgtgaagattgcaaaacgtggcggcgtgaccgcaatggaggcagtgcatgcaagccgcaatgcactgacgggtgcccccctgaacctgacccctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtaatgggggtggcaaacaggctcttgaaaccgtgcaacgactgctcccagttctctgtcaagcccacggcctcaccccggcgcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagaccgtccaacgccttctaccagttctctgtcaagcccacggactaaccccagcgcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagaccgtccaacgccttctaccagttctctgtcaagcccacggactaaccccagcgcaagttgtagcgattgctagtcatgacggtggcaaacaggcccttgagacagtccaacgccttcttccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggctcttgaaaccgtgcaacgactgcttccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggctcttgaaaccgtgcagcgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggcggtcgaccggcgctggagagcattgttgcccagttatctcgccctgatccggcgttggccgcgttgaccaacgaccacctcgtcgccttggcctgcctcggcggacgtcctgcgatggatgcagtgaaaaagggattgccgcacgcgccggaattgatccgtagagtcaatcgccgtattggcgaacgcacatcccatcgcgttgccggatcc BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K1789002_sequence 1 attaaagaggagaaa BBa_K1789023_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatggctccaaagaagaagcgtaaggtagactacaaagaccatgacggtgattataaagatcatgacatcgattacaaggatgacgatgacaagggtaccgtggatctacgcacgctcggctacagccagcagcaacaggagaagatcaaaccgaaggttcgttcgacagtggcgcagcaccacgaggcactggtcggccacgggtttacacacgcgcacatcgttgcgctcagccaacacccggcagcgttagggaccgtcgctgtcacctatcagcatatcatcaccgcgttgccagaggcgacacacgaagatatcgttggcgtcggcaaacagtggtccggcgcacgcgctctggaggccttgctcacggatgcgggagagttgagaggtccaccgttacagttggacacaggccaacttgtgaagattgcaaaacgtggcggcgtgaccgcaatggaggcagtgcatgcaagccgcaatgcactgacgggtgcccccctgaacctgaccccagatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagaccgtccaacgccttctaccagttctctgtcaagcccacggactaaccccagcgcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagacagtccaacgccttctaccagttctctgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggcccttgagacagtccaacgccttcttccagttctttgtcaagctcacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacagcgcctcctccagttctttgtcaagctcacgggctcaccccagatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggcactcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggcggtcgaccggcgctggagagcattgttgcccagttatctcgccctgatccggcgttggccgcgttgaccaacgaccacctcgtcgccttggcctgcctcggcggacgtcctgcgatggatgcagtgaaaaagggattgccgcacgcgccggaattgatccgtagagtcaatcgccgtattggcgaacgcacatcccatcgcgttgccggatcctactagatgtttggaccggatttcccgtttgcatttgatgattggatcgaacatcctaaaggacttgggtcaattccggcagaaaatcacggcgctgaggttgcaattatcggggccggcattgcaggcttagtagcagcatatgaattaatgaagatgggtctgaaacctgtagtctatgaggcctccaaaatgggaggtcgcttacggtcacaggaatttgaaggagcgaagggaattgttgcggaactgggcggtatgcgctttccagtctcctccacagcgttctttcattacgttgataaactgggccttgaaagtcgtccgttccctaaccctttgacggcggccagtggcagcacggtgattgacctggaaggtaccacgtactatgcacagatgttatcagatctcccagccctgttccaagaagtggcggatgcatgggcagacgccttagaatcaggctctcaatttggagatattcaacaggccattcgtgattctgacgtaccgcgtctcaaggaactgtggaacaaactcgtgcctttgtgggacgatcgcactttctatgactttgtagcgacctcaaaggcctttgcgaaattaagcttttaccatcgtgaggttttcggacaagttggttttggtacaggggggtgggattctgattttccaaatagtatgttagaaatctttcgtgttgtgatgacgaattgtgatgagcaccagcatctgattgttggtggcgtacagcaagtgcctgtaggcctgtggtcgcacgtgcctgaacgctgcgcacattggccaaagggtacgtctctttcctcacttcatcggggtgccccgcgcccaggtgtcaaacgtattgcacgtgctgaagatggttcatttacggttacagataattggggcgatacacgtcaatacgctgccgttttgacaacctgtcaatcgtggctgcttacaacgcagattgaatgtgaggaatctttgttttcacagaagatgtggatggcgctggatcgcacgcgttacatgcagtcttctaaaacttttgtgatggttgatcggccgttttggaaggacaaagatccggaaacgggtcgcgatcttatgtccatgaccctcaccgatcgcttaacgcgcggcacatacttatttgataatggcgacgacaagccaggcgttatttgcctgagttactcatggatgagtgacgctttaaaaatgttaccgcaaccgatcgagaaacgcgtcaaattagctttagatgcccttaaaaagatttacccgaaagtggatatcgccgcacgtattattggtgacccgattactgtgagttgggaagcggatccgcatttcctgggggcatttaaaggcgcgttaccgggccattaccgttacaaccagcgcatgtacgcgcattttatgcagcaggacatgccgtcagaacagcgtggcatgtttattgccggtgatgatgtatcttggacaccggcatgggttgagggagcagtacaaacgtcattgaacgcggtgtggggcatcatgaaccatttcggagggaaaacccacgcagagaatccgggtcctggcgacgttttccatgagattggaccgatcgcgctgggggactaataatactagagattaaagaggagaaatactagatggctccaaagaagaagcgtaaggtagactacaaagaccatgacggtgattataaagatcatgacatcgattacaaggatgacgatgacaagggtaccgtggatctacgcacgctcggctacagccagcagcaacaggagaagatcaaaccgaaggttcgttcgacagtggcgcagcaccacgaggcactggtcggccacgggtttacacacgcgcacatcgttgcgctcagccaacacccggcagcgttagggaccgtcgctgtcacctatcagcatatcatcaccgcgttgccagaggcgacacacgaagatatcgttggcgtcggcaaacagtggtccggcgcacgcgctctggaggccttgctcacggatgcgggagagttgagaggtccaccgttacagttggacacaggccaacttgtgaagattgcaaaacgtggcggcgtgaccgcaatggaggcagtgcatgcaagccgcaatgcactgacgggtgcccccctgaacctgacccctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtaatgggggtggcaaacaggctcttgaaaccgtgcaacgactgctcccagttctctgtcaagcccacggcctcaccccggcgcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagaccgtccaacgccttctaccagttctctgtcaagcccacggactaaccccagcgcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagaccgtccaacgccttctaccagttctctgtcaagcccacggactaaccccagcgcaagttgtagcgattgctagtcatgacggtggcaaacaggcccttgagacagtccaacgccttcttccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggctcttgaaaccgtgcaacgactgcttccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggctcttgaaaccgtgcagcgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggcggtcgaccggcgctggagagcattgttgcccagttatctcgccctgatccggcgttggccgcgttgaccaacgaccacctcgtcgccttggcctgcctcggcggacgtcctgcgatggatgcagtgaaaaagggattgccgcacgcgccggaattgatccgtagagtcaatcgccgtattggcgaacgcacatcccatcgcgttgccggatcctactagatgattacgcttgaatctctgtgccgcgcattggcagacgaacagattgccgcagaagaattgcgcgagcgcgcgctggatacagaggctcgcttaacactgttaaattgcttcattcgcgaaggcgacgccgtaagccaattcggtgaagcggatcaggcgcgtaaaggaacttcactttggggggtgcctgtgagttttaaggataacatctgtgttcgcgggctgccactgacggcgggtacccgcggcatgagcggctttattgccgatcaggatgcagcaatcgtgagccagttaaaagcattgggagctgtggtcgcgggcaaaaataacatgcatgagctgtcatttggcgtcacgagcatcaatccacattggggcgcggttggaaacccggtggctccaggatattgcgccggaggttctagcggaggttccgctgcggcggtcgcatctggaattgtcccgctcagcgtcgggacggacacaggcggctcaatccgtattccggcggcattttgcggcattacgggttttcgccctactacaggtcgcttgagtacggcggggatcattccggtcagccacacgaaggattgtgtcggcctcctgacgcgcacagctggcgacgctgaatttgtgtatgggctgctgtcaggaaaacagcagagtttccctcttaatcgcacgggaccttgccgcatcggcctgccggtgagcatgtggtctgacttagatggcgaagtagaacgtgcctgtatcaatgcgctgagcctcttacgcaaaacgggctttgaatttgttgagatcgacgatgcggacattgtggaattgaaccagacattaaccttcacggtgccgctgtacgaatttttcgcggattttgctcaatccttgttgtccttaggttggaaacatggcatccatcatatttttgcacaagtggatgatgcaaatgtgaaaggcattattaatcaccatctgggagagggcgcgattaagccggcacattatctgagttctctccagaatggagagctgctgaaacgcaagatggatgagctttttgcgcgccatcacattaaacttttagggtatccgacggtcccgtgtcgcgtgcctcatctggaccatgcagatcgtccggaattttttagccaggccatccgtaacacagacctcgcaagcaacgcgatgttaccaagcattacaattccggtcggtccggaaggccgcctgccggtcggccttagttttgatgcgcctcgtgctcgtgatgcgtttctcttaagcaatgttagtttgatcgaaaaagtgttaaaaggctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccc BBa_K1789007_sequence 1 tggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccc BBa_K1789008_sequence 1 atggctccaaagaagaagcgtaaggtagactacaaagaccatgacggtgattataaagatcatgacatcgattacaaggatgacgatgacaagggtaccgtggatctacgcacgctcggctacagccagcagcaacaggagaagatcaaaccgaaggttcgttcgacagtggcgcagcaccacgaggcactggtcggccacgggtttacacacgcgcacatcgttgcgctcagccaacacccggcagcgttagggaccgtcgctgtcacctatcagcatatcatcaccgcgttgccagaggcgacacacgaagatatcgttggcgtcggcaaacagtggtccggcgcacgcgctctggaggccttgctcacggatgcgggagagttgagaggtccaccgttacagttggacacaggccaacttgtgaagattgcaaaacgtggcggcgtgaccgcaatggaggcagtgcatgcaagccgcaatgcactgacgggtgcccccctgaacctgaccccagatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagaccgtccaacgccttctaccagttctctgtcaagcccacggactaaccccagcgcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagacagtccaacgccttctaccagttctctgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggcccttgagacagtccaacgccttcttccagttctttgtcaagctcacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacagcgcctcctccagttctttgtcaagctcacgggctcaccccagatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggcactcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggcggtcgaccggcgctggagagcattgttgcccagttatctcgccctgatccggcgttggccgcgttgaccaacgaccacctcgtcgccttggcctgcctcggcggacgtcctgcgatggatgcagtgaaaaagggattgccgcacgcgccggaattgatccgtagagtcaatcgccgtattggcgaacgcacatcccatcgcgttgccggatcc BBa_K1789012_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaa BBa_K1789000_sequence 1 atgtttggaccggatttcccgtttgcatttgatgattggatcgaacatcctaaaggacttgggtcaattccggcagaaaatcacggcgctgaggttgcaattatcggggccggcattgcaggcttagtagcagcatatgaattaatgaagatgggtctgaaacctgtagtctatgaggcctccaaaatgggaggtcgcttacggtcacaggaatttgaaggagcgaagggaattgttgcggaactgggcggtatgcgctttccagtctcctccacagcgttctttcattacgttgataaactgggccttgaaagtcgtccgttccctaaccctttgacggcggccagtggcagcacggtgattgacctggaaggtaccacgtactatgcacagatgttatcagatctcccagccctgttccaagaagtggcggatgcatgggcagacgccttagaatcaggctctcaatttggagatattcaacaggccattcgtgattctgacgtaccgcgtctcaaggaactgtggaacaaactcgtgcctttgtgggacgatcgcactttctatgactttgtagcgacctcaaaggcctttgcgaaattaagcttttaccatcgtgaggttttcggacaagttggttttggtacaggggggtgggattctgattttccaaatagtatgttagaaatctttcgtgttgtgatgacgaattgtgatgagcaccagcatctgattgttggtggcgtacagcaagtgcctgtaggcctgtggtcgcacgtgcctgaacgctgcgcacattggccaaagggtacgtctctttcctcacttcatcggggtgccccgcgcccaggtgtcaaacgtattgcacgtgctgaagatggttcatttacggttacagataattggggcgatacacgtcaatacgctgccgttttgacaacctgtcaatcgtggctgcttacaacgcagattgaatgtgaggaatctttgttttcacagaagatgtggatggcgctggatcgcacgcgttacatgcagtcttctaaaacttttgtgatggttgatcggccgttttggaaggacaaagatccggaaacgggtcgcgatcttatgtccatgaccctcaccgatcgcttaacgcgcggcacatacttatttgataatggcgacgacaagccaggcgttatttgcctgagttactcatggatgagtgacgctttaaaaatgttaccgcaaccgatcgagaaacgcgtcaaattagctttagatgcccttaaaaagatttacccgaaagtggatatcgccgcacgtattattggtgacccgattactgtgagttgggaagcggatccgcatttcctgggggcatttaaaggcgcgttaccgggccattaccgttacaaccagcgcatgtacgcgcattttatgcagcaggacatgccgtcagaacagcgtggcatgtttattgccggtgatgatgtatcttggacaccggcatgggttgagggagcagtacaaacgtcattgaacgcggtgtggggcatcatgaaccatttcggagggaaaacccacgcagagaatccgggtcctggcgacgttttccatgagattggaccgatcgcgctgggggactaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z