BBa_K1789009
1
BBa_K1789009
TALE2
2015-09-13T11:00:00Z
2015-09-14T07:20:22Z
This part is built and standardized by ourselves using GATEWAY technique.
This is a engineered TAL effector that can bind with the DNA sequence 5'-GATAAACACCTTTC-3'
false
false
_2214_
21134
21134
9
false
The part sequence should be able to correctly and efficiently bind with corresponding DNA, so we used bioinformatics tools to help us designing this sequence.
false
Xinyuan Qiu
annotation2454121
1
TALE2
range2454121
1
1
2088
annotation2454120
1
DNA Binding Domain
range2454120
1
514
1872
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961227
1
start
range1961227
1
173
173
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961224
1
-35
range1961224
1
137
142
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
BBa_K1789023
1
BBa_K1789023
IAA(+/+)S3
2015-09-13T11:00:00Z
2015-09-14T07:35:36Z
We obtained it through the contribution of our basic subparts.
This is a device which contains the sequence of TALE based IAAM, TALE based IAAH and scaffold 3. Through this device, we can test and verify that our scaffold can strengthen the function of IAA.
false
false
_2214_
26296
26296
9
false
This is the experimental group of our project. Our theory is feasible if the functional parameter of this group is stronger than the group of negative control.
false
Chushu Zhu
component2454195
1
BBa_B0015
component2454198
1
BBa_K1789007
component2454183
1
BBa_K1789002
component2454179
1
BBa_K1789008
component2454188
1
BBa_K1789001
component2454186
1
BBa_K1789009
component2454176
1
BBa_K1789012
component2454181
1
BBa_K1789000
annotation2454188
1
BBa_K1789001
range2454188
1
6084
7418
annotation2454198
1
BBa_K1789007
range2454198
1
7564
8245
annotation2454181
1
BBa_K1789000
range2454181
1
2323
3960
annotation2454186
1
BBa_K1789009
range2454186
1
3990
6077
annotation2454179
1
BBa_K1789008
range2454179
1
230
2316
annotation2454195
1
BBa_B0015
range2454195
1
7427
7555
annotation2454176
1
BBa_K1789012
range2454176
1
1
223
annotation2454183
1
BBa_K1789002
range2454183
1
3969
3983
BBa_K1789007
1
BBa_K1789007
SCAF3
2015-09-13T11:00:00Z
2015-09-18T04:48:41Z
This part is synthesized by company
This part is a scaffold system contains the recognizing motifs of TALE1 and TALE2
false
false
_2214_
21134
21134
9
false
We designed this sequence to provide sites for our engineered TALE proteins to bind
false
Xinyuan Qiu
annotation2454075
1
TALE1 BINDING SITE
range2454075
1
1
14
annotation2470998
1
SCAF3
range2470998
1
1
682
annotation2454077
1
TALE2 BINDING SITE
range2454077
1
32
46
BBa_K1789002
1
BBa_K1789002
RBS30
2015-09-13T11:00:00Z
2015-09-14T06:03:14Z
The part sequence was gained from the registry, the part is annealed from the ssDNA synthesized by Life(R).
This part is another copy of the RBS30 (BBa_B0030). It is a Strong RBS based on Ron Weiss thesis.
false
false
_2214_
21134
21134
9
false
The reason why we built this part by ourselves is that the copy of B0030 in the 2015 DNA distribution we received is sequenced as incorrect. And same problem also occurred in several other teams.
false
Xinyuan Qiu
annotation2454064
1
RBS30
range2454064
1
1
15
BBa_K1789008
1
BBa_K1789008
TALE1
2015-09-13T11:00:00Z
2015-09-18T02:27:57Z
This part is built by NJU_CHINA using GATEWAY technique. We standardized this part by ourselves.
This is a engineered TAL effector that can bind with the DNA sequence 5'-GGAGGCACCGGTGG-3'
false
false
_2214_
21134
21134
9
false
The part sequence should be able to correctly and efficiently bind with corresponding DNA, so we used bioinformatics tools to help us designing this sequence.
false
Xinyuan Qiu
annotation2454114
1
TALE1
range2454114
1
1
2087
annotation2454113
1
DNA Binding domain
range2454113
1
508
1872
BBa_K1789001
1
BBa_K1789001
IaaH
2015-09-13T11:00:00Z
2015-09-14T05:49:28Z
This part is gained from the part BBa_K515100 by PCR.
This part is the coding sequence of the enzyme Indoleacetimide hydrolase Iaa H. This enzyme catalyses the hydrolysis of indoleacetamide to indoleacetate and ammonia. This part is gathered from the 2-step pathway in Pseudomonas savastanoi producing the auxin, indole-3-acetic acid.
false
false
_2214_
21134
21134
9
false
In order to get the whole sequence of IAAH, we removed the RBS and other irrelevant sequences in K515100 when designing the PCR primers.
false
Xinyuan Qiu
annotation2454035
1
IaaH
range2454035
1
1
1335
BBa_K1789012
1
BBa_K1789012
P+R
2015-09-13T11:00:00Z
2015-09-14T07:22:47Z
It is formed by our basic parts.
This part is a promoter. It has a strong RBS restriction site following it which can guarantee the high efficiency of our project.
false
false
_2214_
26298
26298
9
false
We want to construct a promoter which has RBS30 itself for RBS30 is small. If we supply a promoter with RBS, the efficiency of building will be high.
false
Jiaqi Sun
component2454130
1
BBa_K1789002
component2454122
1
BBa_R0010
annotation2454130
1
BBa_K1789002
range2454130
1
209
223
annotation2454122
1
BBa_R0010
range2454122
1
1
200
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_K1789000
1
BBa_K1789000
IaaM
2015-09-11T11:00:00Z
2015-09-12T02:27:07Z
This part is gained from BBa_K515100 by PCR. The IAA producing pathway originates from Pseudomonas savastanoi.
This part is the coding sequence of tryptophan-2-mono-oxygenase. This enzyme catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide which acts as an intermediate to the production of indole-3-acetic acid in the IAM pathway.
false
false
_2214_
21134
21134
9
false
The reason why er designed this part is that we needed IAA producing pathway as a reporter in our system, and the IAAM and IAAH CDS needed to be fused with the coding sequence of the TAL effector we engineered. So we used the part BBa_K515100, which contains the CDS of IaaM and IaaH, and used PCR to eliminate the RBS sequence from the 5' region of the CDS.
false
Xinyuan Qiu
annotation2452420
1
IaaM
range2452420
1
1
1638
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1789001_sequence
1
atgattacgcttgaatctctgtgccgcgcattggcagacgaacagattgccgcagaagaattgcgcgagcgcgcgctggatacagaggctcgcttaacactgttaaattgcttcattcgcgaaggcgacgccgtaagccaattcggtgaagcggatcaggcgcgtaaaggaacttcactttggggggtgcctgtgagttttaaggataacatctgtgttcgcgggctgccactgacggcgggtacccgcggcatgagcggctttattgccgatcaggatgcagcaatcgtgagccagttaaaagcattgggagctgtggtcgcgggcaaaaataacatgcatgagctgtcatttggcgtcacgagcatcaatccacattggggcgcggttggaaacccggtggctccaggatattgcgccggaggttctagcggaggttccgctgcggcggtcgcatctggaattgtcccgctcagcgtcgggacggacacaggcggctcaatccgtattccggcggcattttgcggcattacgggttttcgccctactacaggtcgcttgagtacggcggggatcattccggtcagccacacgaaggattgtgtcggcctcctgacgcgcacagctggcgacgctgaatttgtgtatgggctgctgtcaggaaaacagcagagtttccctcttaatcgcacgggaccttgccgcatcggcctgccggtgagcatgtggtctgacttagatggcgaagtagaacgtgcctgtatcaatgcgctgagcctcttacgcaaaacgggctttgaatttgttgagatcgacgatgcggacattgtggaattgaaccagacattaaccttcacggtgccgctgtacgaatttttcgcggattttgctcaatccttgttgtccttaggttggaaacatggcatccatcatatttttgcacaagtggatgatgcaaatgtgaaaggcattattaatcaccatctgggagagggcgcgattaagccggcacattatctgagttctctccagaatggagagctgctgaaacgcaagatggatgagctttttgcgcgccatcacattaaacttttagggtatccgacggtcccgtgtcgcgtgcctcatctggaccatgcagatcgtccggaattttttagccaggccatccgtaacacagacctcgcaagcaacgcgatgttaccaagcattacaattccggtcggtccggaaggccgcctgccggtcggccttagttttgatgcgcctcgtgctcgtgatgcgtttctcttaagcaatgttagtttgatcgaaaaagtgttaaaaggctaataa
BBa_K1789009_sequence
1
atggctccaaagaagaagcgtaaggtagactacaaagaccatgacggtgattataaagatcatgacatcgattacaaggatgacgatgacaagggtaccgtggatctacgcacgctcggctacagccagcagcaacaggagaagatcaaaccgaaggttcgttcgacagtggcgcagcaccacgaggcactggtcggccacgggtttacacacgcgcacatcgttgcgctcagccaacacccggcagcgttagggaccgtcgctgtcacctatcagcatatcatcaccgcgttgccagaggcgacacacgaagatatcgttggcgtcggcaaacagtggtccggcgcacgcgctctggaggccttgctcacggatgcgggagagttgagaggtccaccgttacagttggacacaggccaacttgtgaagattgcaaaacgtggcggcgtgaccgcaatggaggcagtgcatgcaagccgcaatgcactgacgggtgcccccctgaacctgacccctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtaatgggggtggcaaacaggctcttgaaaccgtgcaacgactgctcccagttctctgtcaagcccacggcctcaccccggcgcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagaccgtccaacgccttctaccagttctctgtcaagcccacggactaaccccagcgcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagaccgtccaacgccttctaccagttctctgtcaagcccacggactaaccccagcgcaagttgtagcgattgctagtcatgacggtggcaaacaggcccttgagacagtccaacgccttcttccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggctcttgaaaccgtgcaacgactgcttccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggctcttgaaaccgtgcagcgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggcggtcgaccggcgctggagagcattgttgcccagttatctcgccctgatccggcgttggccgcgttgaccaacgaccacctcgtcgccttggcctgcctcggcggacgtcctgcgatggatgcagtgaaaaagggattgccgcacgcgccggaattgatccgtagagtcaatcgccgtattggcgaacgcacatcccatcgcgttgccggatcc
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_K1789002_sequence
1
attaaagaggagaaa
BBa_K1789023_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatggctccaaagaagaagcgtaaggtagactacaaagaccatgacggtgattataaagatcatgacatcgattacaaggatgacgatgacaagggtaccgtggatctacgcacgctcggctacagccagcagcaacaggagaagatcaaaccgaaggttcgttcgacagtggcgcagcaccacgaggcactggtcggccacgggtttacacacgcgcacatcgttgcgctcagccaacacccggcagcgttagggaccgtcgctgtcacctatcagcatatcatcaccgcgttgccagaggcgacacacgaagatatcgttggcgtcggcaaacagtggtccggcgcacgcgctctggaggccttgctcacggatgcgggagagttgagaggtccaccgttacagttggacacaggccaacttgtgaagattgcaaaacgtggcggcgtgaccgcaatggaggcagtgcatgcaagccgcaatgcactgacgggtgcccccctgaacctgaccccagatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagaccgtccaacgccttctaccagttctctgtcaagcccacggactaaccccagcgcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagacagtccaacgccttctaccagttctctgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggcccttgagacagtccaacgccttcttccagttctttgtcaagctcacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacagcgcctcctccagttctttgtcaagctcacgggctcaccccagatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggcactcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggcggtcgaccggcgctggagagcattgttgcccagttatctcgccctgatccggcgttggccgcgttgaccaacgaccacctcgtcgccttggcctgcctcggcggacgtcctgcgatggatgcagtgaaaaagggattgccgcacgcgccggaattgatccgtagagtcaatcgccgtattggcgaacgcacatcccatcgcgttgccggatcctactagatgtttggaccggatttcccgtttgcatttgatgattggatcgaacatcctaaaggacttgggtcaattccggcagaaaatcacggcgctgaggttgcaattatcggggccggcattgcaggcttagtagcagcatatgaattaatgaagatgggtctgaaacctgtagtctatgaggcctccaaaatgggaggtcgcttacggtcacaggaatttgaaggagcgaagggaattgttgcggaactgggcggtatgcgctttccagtctcctccacagcgttctttcattacgttgataaactgggccttgaaagtcgtccgttccctaaccctttgacggcggccagtggcagcacggtgattgacctggaaggtaccacgtactatgcacagatgttatcagatctcccagccctgttccaagaagtggcggatgcatgggcagacgccttagaatcaggctctcaatttggagatattcaacaggccattcgtgattctgacgtaccgcgtctcaaggaactgtggaacaaactcgtgcctttgtgggacgatcgcactttctatgactttgtagcgacctcaaaggcctttgcgaaattaagcttttaccatcgtgaggttttcggacaagttggttttggtacaggggggtgggattctgattttccaaatagtatgttagaaatctttcgtgttgtgatgacgaattgtgatgagcaccagcatctgattgttggtggcgtacagcaagtgcctgtaggcctgtggtcgcacgtgcctgaacgctgcgcacattggccaaagggtacgtctctttcctcacttcatcggggtgccccgcgcccaggtgtcaaacgtattgcacgtgctgaagatggttcatttacggttacagataattggggcgatacacgtcaatacgctgccgttttgacaacctgtcaatcgtggctgcttacaacgcagattgaatgtgaggaatctttgttttcacagaagatgtggatggcgctggatcgcacgcgttacatgcagtcttctaaaacttttgtgatggttgatcggccgttttggaaggacaaagatccggaaacgggtcgcgatcttatgtccatgaccctcaccgatcgcttaacgcgcggcacatacttatttgataatggcgacgacaagccaggcgttatttgcctgagttactcatggatgagtgacgctttaaaaatgttaccgcaaccgatcgagaaacgcgtcaaattagctttagatgcccttaaaaagatttacccgaaagtggatatcgccgcacgtattattggtgacccgattactgtgagttgggaagcggatccgcatttcctgggggcatttaaaggcgcgttaccgggccattaccgttacaaccagcgcatgtacgcgcattttatgcagcaggacatgccgtcagaacagcgtggcatgtttattgccggtgatgatgtatcttggacaccggcatgggttgagggagcagtacaaacgtcattgaacgcggtgtggggcatcatgaaccatttcggagggaaaacccacgcagagaatccgggtcctggcgacgttttccatgagattggaccgatcgcgctgggggactaataatactagagattaaagaggagaaatactagatggctccaaagaagaagcgtaaggtagactacaaagaccatgacggtgattataaagatcatgacatcgattacaaggatgacgatgacaagggtaccgtggatctacgcacgctcggctacagccagcagcaacaggagaagatcaaaccgaaggttcgttcgacagtggcgcagcaccacgaggcactggtcggccacgggtttacacacgcgcacatcgttgcgctcagccaacacccggcagcgttagggaccgtcgctgtcacctatcagcatatcatcaccgcgttgccagaggcgacacacgaagatatcgttggcgtcggcaaacagtggtccggcgcacgcgctctggaggccttgctcacggatgcgggagagttgagaggtccaccgttacagttggacacaggccaacttgtgaagattgcaaaacgtggcggcgtgaccgcaatggaggcagtgcatgcaagccgcaatgcactgacgggtgcccccctgaacctgacccctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtaatgggggtggcaaacaggctcttgaaaccgtgcaacgactgctcccagttctctgtcaagcccacggcctcaccccggcgcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagaccgtccaacgccttctaccagttctctgtcaagcccacggactaaccccagcgcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagaccgtccaacgccttctaccagttctctgtcaagcccacggactaaccccagcgcaagttgtagcgattgctagtcatgacggtggcaaacaggcccttgagacagtccaacgccttcttccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggctcttgaaaccgtgcaacgactgcttccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggctcttgaaaccgtgcagcgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggcggtcgaccggcgctggagagcattgttgcccagttatctcgccctgatccggcgttggccgcgttgaccaacgaccacctcgtcgccttggcctgcctcggcggacgtcctgcgatggatgcagtgaaaaagggattgccgcacgcgccggaattgatccgtagagtcaatcgccgtattggcgaacgcacatcccatcgcgttgccggatcctactagatgattacgcttgaatctctgtgccgcgcattggcagacgaacagattgccgcagaagaattgcgcgagcgcgcgctggatacagaggctcgcttaacactgttaaattgcttcattcgcgaaggcgacgccgtaagccaattcggtgaagcggatcaggcgcgtaaaggaacttcactttggggggtgcctgtgagttttaaggataacatctgtgttcgcgggctgccactgacggcgggtacccgcggcatgagcggctttattgccgatcaggatgcagcaatcgtgagccagttaaaagcattgggagctgtggtcgcgggcaaaaataacatgcatgagctgtcatttggcgtcacgagcatcaatccacattggggcgcggttggaaacccggtggctccaggatattgcgccggaggttctagcggaggttccgctgcggcggtcgcatctggaattgtcccgctcagcgtcgggacggacacaggcggctcaatccgtattccggcggcattttgcggcattacgggttttcgccctactacaggtcgcttgagtacggcggggatcattccggtcagccacacgaaggattgtgtcggcctcctgacgcgcacagctggcgacgctgaatttgtgtatgggctgctgtcaggaaaacagcagagtttccctcttaatcgcacgggaccttgccgcatcggcctgccggtgagcatgtggtctgacttagatggcgaagtagaacgtgcctgtatcaatgcgctgagcctcttacgcaaaacgggctttgaatttgttgagatcgacgatgcggacattgtggaattgaaccagacattaaccttcacggtgccgctgtacgaatttttcgcggattttgctcaatccttgttgtccttaggttggaaacatggcatccatcatatttttgcacaagtggatgatgcaaatgtgaaaggcattattaatcaccatctgggagagggcgcgattaagccggcacattatctgagttctctccagaatggagagctgctgaaacgcaagatggatgagctttttgcgcgccatcacattaaacttttagggtatccgacggtcccgtgtcgcgtgcctcatctggaccatgcagatcgtccggaattttttagccaggccatccgtaacacagacctcgcaagcaacgcgatgttaccaagcattacaattccggtcggtccggaaggccgcctgccggtcggccttagttttgatgcgcctcgtgctcgtgatgcgtttctcttaagcaatgttagtttgatcgaaaaagtgttaaaaggctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccc
BBa_K1789007_sequence
1
tggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccctggaggcaccggtggacagtagctacaaccctgataaacacctttcacagtagctacaaccc
BBa_K1789008_sequence
1
atggctccaaagaagaagcgtaaggtagactacaaagaccatgacggtgattataaagatcatgacatcgattacaaggatgacgatgacaagggtaccgtggatctacgcacgctcggctacagccagcagcaacaggagaagatcaaaccgaaggttcgttcgacagtggcgcagcaccacgaggcactggtcggccacgggtttacacacgcgcacatcgttgcgctcagccaacacccggcagcgttagggaccgtcgctgtcacctatcagcatatcatcaccgcgttgccagaggcgacacacgaagatatcgttggcgtcggcaaacagtggtccggcgcacgcgctctggaggccttgctcacggatgcgggagagttgagaggtccaccgttacagttggacacaggccaacttgtgaagattgcaaaacgtggcggcgtgaccgcaatggaggcagtgcatgcaagccgcaatgcactgacgggtgcccccctgaacctgaccccagatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagaccgtccaacgccttctaccagttctctgtcaagcccacggactaaccccagcgcaagttgtagcgattgctagtaatattggtggcaaacaggcacttgagacggttcagcgcctccttccagttctttgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggctcttgagacagtccaacgccttctaccagttctctgtcaagctcacggactcaccccagatcaagttgtagcgattgctagtcatgacggtggcaaacaggcccttgagacagtccaacgccttcttccagttctttgtcaagctcacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacagcgcctcctccagttctttgtcaagctcacgggctcaccccagatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaatgggggtggcaaacaggcactcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggtggcaaacaggctctcgaaaccgtacaacgactcctcccagttctctgtcaagcccacggactaactcctgatcaagttgtagcgattgctagtaacaatggcggtcgaccggcgctggagagcattgttgcccagttatctcgccctgatccggcgttggccgcgttgaccaacgaccacctcgtcgccttggcctgcctcggcggacgtcctgcgatggatgcagtgaaaaagggattgccgcacgcgccggaattgatccgtagagtcaatcgccgtattggcgaacgcacatcccatcgcgttgccggatcc
BBa_K1789012_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaa
BBa_K1789000_sequence
1
atgtttggaccggatttcccgtttgcatttgatgattggatcgaacatcctaaaggacttgggtcaattccggcagaaaatcacggcgctgaggttgcaattatcggggccggcattgcaggcttagtagcagcatatgaattaatgaagatgggtctgaaacctgtagtctatgaggcctccaaaatgggaggtcgcttacggtcacaggaatttgaaggagcgaagggaattgttgcggaactgggcggtatgcgctttccagtctcctccacagcgttctttcattacgttgataaactgggccttgaaagtcgtccgttccctaaccctttgacggcggccagtggcagcacggtgattgacctggaaggtaccacgtactatgcacagatgttatcagatctcccagccctgttccaagaagtggcggatgcatgggcagacgccttagaatcaggctctcaatttggagatattcaacaggccattcgtgattctgacgtaccgcgtctcaaggaactgtggaacaaactcgtgcctttgtgggacgatcgcactttctatgactttgtagcgacctcaaaggcctttgcgaaattaagcttttaccatcgtgaggttttcggacaagttggttttggtacaggggggtgggattctgattttccaaatagtatgttagaaatctttcgtgttgtgatgacgaattgtgatgagcaccagcatctgattgttggtggcgtacagcaagtgcctgtaggcctgtggtcgcacgtgcctgaacgctgcgcacattggccaaagggtacgtctctttcctcacttcatcggggtgccccgcgcccaggtgtcaaacgtattgcacgtgctgaagatggttcatttacggttacagataattggggcgatacacgtcaatacgctgccgttttgacaacctgtcaatcgtggctgcttacaacgcagattgaatgtgaggaatctttgttttcacagaagatgtggatggcgctggatcgcacgcgttacatgcagtcttctaaaacttttgtgatggttgatcggccgttttggaaggacaaagatccggaaacgggtcgcgatcttatgtccatgaccctcaccgatcgcttaacgcgcggcacatacttatttgataatggcgacgacaagccaggcgttatttgcctgagttactcatggatgagtgacgctttaaaaatgttaccgcaaccgatcgagaaacgcgtcaaattagctttagatgcccttaaaaagatttacccgaaagtggatatcgccgcacgtattattggtgacccgattactgtgagttgggaagcggatccgcatttcctgggggcatttaaaggcgcgttaccgggccattaccgttacaaccagcgcatgtacgcgcattttatgcagcaggacatgccgtcagaacagcgtggcatgtttattgccggtgatgatgtatcttggacaccggcatgggttgagggagcagtacaaacgtcattgaacgcggtgtggggcatcatgaaccatttcggagggaaaacccacgcagagaatccgggtcctggcgacgttttccatgagattggaccgatcgcgctgggggactaataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z