LOCUS       BBa_K1791001             824 bp    DNA     linear       26-Nov-2024
DEFINITION  Standard RBS expressing MS2 Coat protein ribozyme affinity 
            purification (RAP)
ACCESSION   BBa_K1791001
VERSION     BBa_K1791001.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     CDS             50..457
                     /label=BBa_K1791003
     stem_loop       477..520
                     /label=stem_loop
     misc_feature    466..545
                     /label=BBa_B0010
     terminator      466..545
                     /label=BBa_B0010
     stem_loop       561..580
                     /label=T7 TE
     misc_feature    587..587
                     /label=stop
     polyA_site      581..594
                     /label=polya
     misc_feature    554..594
                     /label=BBa_B0012
     terminator      554..594
                     /label=BBa_B0012
     terminator      466..594
                     /label=BBa_B0015
     misc_feature    603..608
                     /label=BBa_K1791007
     misc_feature    617..765
                     /label=BBa_K1791005
     misc_feature    774..779
                     /label=BBa_K1791008
     misc_feature    788..824
                     /label=BBa_K1791006
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggcaagcaa
       61 cttcacgcag tttgtgctgg tcgacaatgg cggtaccggt gacgttacgg ttgccccaag
      121 caacttcgcc aacggtgtcg ccgagtggat ttccagcaac tctcgctccc aggcatataa
      181 agtaacatgt agcgtgcgcc agtcaagtgc acagaatcgc aagtacacca tcaaagtgga
      241 ggtgccgaaa gtcgcaaccc agacggtggg cggtgttgaa cttccggttg ccgcgtggcg
      301 ctcctatctg aatatggaac tgaccatccc gatctttgct accaattccg attgcgaatt
      361 gattgtcaaa gcgatgcagg ggttactgaa ggacggaaat ccgattccaa gtgcaattgc
      421 tgctaacagc ggaatctatc accaccacca tcatcattac tagagccagg catcaaataa
      481 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      541 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
      601 agggatccta ctagagtaat acgactcact atagggagaa aacaaacaaa gctgtcaccg
      661 gatgtgcttt ccggtctgat gagtccgtgt tgctgatacc agcatcgtct tgatgccctt
      721 ggcagcagtg gacgaggacg aaacagcaaa aagaaaaata aaaaatacta gagggtacct
      781 actagaggaa aagaaaacat gaggatcacc catgtcaaaa gaaa
//