BBa_K1791001
type: ComponentDefinition
persistent identity: BBa K1791001
display id: BBa_K1791001
version: 1
was generated by: iGEM to SBOL conversion
description: Standard RBS expressing MS2 Coat protein ribozyme affinity purification (RAP)
created: 2015-09-16T11:00:00Z
modified: 2015-09-23T01:30:44Z
mutable provenance: This is an amalgamation of several biobricks an MS2 coat protein and the theophylline ribozyme (BBa_K1791000)
owned by: james
owned by: myers
mutable description: Standard RBS expressing MS2 Coat protein ribozyme affinity purification (RAP). This part utilizes the expression of C-terminal 6X His tagged fusion MS2 coat protein under a T7 promoter and a standard shine dalgarno sequence for expression of MS2. Downstream there is a T7 promoter followed by a theophylline ribozyme and a MS2 apatamer at the 3' end. Co-expression of the two modules results in MS2 binding to the 3' end of the theophylline ribozyme transcript allowing for the MS2-RNA purification by nickel affinity chromatography. The RNA can be eluted from the complex by the addition of theophylline.
discontinued: false
dominant: false
experience: experience/None
group u list: _2216_
m user id: 17031
owner id: 17031
owning group id: 9
status: status/Planning
bookmark: true
mutable notes: We had to consider MS2 expression levels relative to the theophylline ribozyme transcript and assessed how this affects total RNA yield. In order to test this we developed a second part with depressed MS2 expression (BBa_K1791002)
star: false
creator: Graeme Glaister
type: DnaRegion
role: SO:0000110
role: partType/DNA
component: BBa_K1791008
component: BBa_K1791005
component: BBa_K1791006
component: BBa_I719005
component: BBa_K1791003
component: BBa_K1791007
component: BBa_B0034
component: BBa_B0015
sequence annotation: BBa_I719005
sequence annotation: BBa_B0034
sequence annotation: BBa_K1791003
sequence annotation: BBa_B0015
sequence annotation: BBa_K1791007
sequence annotation: BBa_K1791005
sequence annotation: BBa_K1791008
sequence annotation: BBa_K1791006
sequence: https://synbiohub.org/public/igem/BBa_K1791001_sequence/1

BBa_K1791008
type: Component
persistent identity: component2476613
display id: component2476613
version: 1
top level: BBa_K1791001
definition: BBa_K1791008
access: public

BBa_K1791005
type: Component
persistent identity: component2476612
display id: component2476612
version: 1
top level: BBa_K1791001
definition: BBa_K1791005
access: public

BBa_K1791006
type: Component
persistent identity: component2476614
display id: component2476614
version: 1
top level: BBa_K1791001
definition: BBa_K1791006
access: public

BBa_I719005
type: Component
persistent identity: component2476600
display id: component2476600
version: 1
top level: BBa_K1791001
definition: pT7
access: public

BBa_K1791003
type: Component
persistent identity: component2476603
display id: component2476603
version: 1
top level: BBa_K1791001
definition: BBa_K1791003
access: public

BBa_K1791007
type: Component
persistent identity: component2476611
display id: component2476611
version: 1
top level: BBa_K1791001
definition: BBa_K1791007
access: public

BBa_B0034
type: Component
persistent identity: component2476602
display id: component2476602
version: 1
top level: BBa_K1791001
definition: BBa_B0034
access: public

BBa_B0015
type: Component
persistent identity: component2476610
display id: component2476610
version: 1
top level: BBa_K1791001
definition: BBa_B0015
access: public

BBa_I719005
type: SequenceAnnotation
persistent identity: annotation2476600
display id: annotation2476600
version: 1
top level: BBa_K1791001
location: https://synbiohub.org/public/igem/BBa_K1791001/annotation2476600/range2476600/1
component: BBa_I719005

https://synbiohub.org/public/igem/BBa_K1791001/annotation2476600/range2476600/1
type: Range
persistent identity: range2476600
display id: range2476600
version: 1
top level: BBa_K1791001
direction: direction/reverse
start: 1
end: 23
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2476602
display id: annotation2476602
version: 1
top level: BBa_K1791001
location: https://synbiohub.org/public/igem/BBa_K1791001/annotation2476602/range2476602/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1791001/annotation2476602/range2476602/1
type: Range
persistent identity: range2476602
display id: range2476602
version: 1
top level: BBa_K1791001
direction: direction/reverse
start: 32
end: 43
orientation: inline

BBa_K1791003
type: SequenceAnnotation
persistent identity: annotation2476603
display id: annotation2476603
version: 1
top level: BBa_K1791001
location: https://synbiohub.org/public/igem/BBa_K1791001/annotation2476603/range2476603/1
component: BBa_K1791003

https://synbiohub.org/public/igem/BBa_K1791001/annotation2476603/range2476603/1
type: Range
persistent identity: range2476603
display id: range2476603
version: 1
top level: BBa_K1791001
direction: direction/reverse
start: 50
end: 457
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2476610
display id: annotation2476610
version: 1
top level: BBa_K1791001
location: https://synbiohub.org/public/igem/BBa_K1791001/annotation2476610/range2476610/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1791001/annotation2476610/range2476610/1
type: Range
persistent identity: range2476610
display id: range2476610
version: 1
top level: BBa_K1791001
direction: direction/reverse
start: 466
end: 594
orientation: inline

BBa_K1791007
type: SequenceAnnotation
persistent identity: annotation2476611
display id: annotation2476611
version: 1
top level: BBa_K1791001
location: https://synbiohub.org/public/igem/BBa_K1791001/annotation2476611/range2476611/1
component: BBa_K1791007

https://synbiohub.org/public/igem/BBa_K1791001/annotation2476611/range2476611/1
type: Range
persistent identity: range2476611
display id: range2476611
version: 1
top level: BBa_K1791001
direction: direction/reverse
start: 603
end: 608
orientation: inline

BBa_K1791005
type: SequenceAnnotation
persistent identity: annotation2476612
display id: annotation2476612
version: 1
top level: BBa_K1791001
location: https://synbiohub.org/public/igem/BBa_K1791001/annotation2476612/range2476612/1
component: BBa_K1791005

https://synbiohub.org/public/igem/BBa_K1791001/annotation2476612/range2476612/1
type: Range
persistent identity: range2476612
display id: range2476612
version: 1
top level: BBa_K1791001
direction: direction/reverse
start: 617
end: 765
orientation: inline

BBa_K1791008
type: SequenceAnnotation
persistent identity: annotation2476613
display id: annotation2476613
version: 1
top level: BBa_K1791001
location: https://synbiohub.org/public/igem/BBa_K1791001/annotation2476613/range2476613/1
component: BBa_K1791008

https://synbiohub.org/public/igem/BBa_K1791001/annotation2476613/range2476613/1
type: Range
persistent identity: range2476613
display id: range2476613
version: 1
top level: BBa_K1791001
direction: direction/reverse
start: 774
end: 779
orientation: inline

BBa_K1791006
type: SequenceAnnotation
persistent identity: annotation2476614
display id: annotation2476614
version: 1
top level: BBa_K1791001
location: https://synbiohub.org/public/igem/BBa_K1791001/annotation2476614/range2476614/1
component: BBa_K1791006

https://synbiohub.org/public/igem/BBa_K1791001/annotation2476614/range2476614/1
type: Range
persistent identity: range2476614
display id: range2476614
version: 1
top level: BBa_K1791001
direction: direction/reverse
start: 788
end: 824
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

pT7
type: ComponentDefinition
persistent identity: BBa I719005
display id: BBa_I719005
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Promoter
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:07:53Z
mutable provenance: ---
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Just a T7 Promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _128_
m user id: 0
owner id: 2097
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None
star: true
creator: Imperial 2007
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_I719005_sequence/1

BBa_K1791003
type: ComponentDefinition
persistent identity: BBa K1791003
display id: BBa_K1791003
version: 1
was generated by: iGEM to SBOL conversion
description: 6X end terminally His tagged MS2 coat protein
created: 2015-09-22T11:00:00Z
modified: 2015-09-22T11:10:08Z
mutable provenance: RefSeq NP_040648.1. NC_001417.2.
owned by: james
owned by: myers
mutable description: MS2 coat protein can be easily purified via a nickel sepharose purification if this part is used downstream of a promoter and RBS.
discontinued: false
dominant: false
group u list: _2216_
m user id: 17031
owner id: 17031
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: optimized for expression in E.coli
star: false
creator: Graeme Glaister
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K1791003_sequence/1

BBa_K1791005
type: ComponentDefinition
persistent identity: BBa K1791005
display id: BBa_K1791005
version: 1
was generated by: iGEM to SBOL conversion
description: T7 promoter fused with a theophylline inducible ribozyme (aptazyme)
created: 2015-09-22T11:00:00Z
modified: 2015-09-22T11:17:58Z
mutable provenance: Win, M.N., Smolke C.D. 2007 and BBa_I719005
owned by: james
owned by: myers
mutable description: This part was developed in order to increase the sequence space flexibility in dsRNA generated using BBa_K1791001 and BBa_K1791002
discontinued: false
dominant: false
group u list: _2216_
m user id: 17031
owner id: 17031
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: by changing the T7 promoter sequence (BBa_I719005) slightly after the GGG trinulceotide we get an increase in the potential for generating RNA with our composite parts BBa_K1791001 and BBa_K1791002.
star: false
creator: Graeme Glaister
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K1791005_sequence/1

BBa_K1791006
type: ComponentDefinition
persistent identity: BBa K1791006
display id: BBa_K1791006
version: 1
was generated by: iGEM to SBOL conversion
description: MS2 binding domain
created: 2015-09-22T11:00:00Z
modified: 2015-09-22T11:21:29Z
mutable provenance: genomic sequence in E.coli
owned by: james
owned by: myers
mutable description: This is an aptamer designed to bind MS2 coat protein upon transcription.
discontinued: false
dominant: false
group u list: _2216_
m user id: 17031
owner id: 17031
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Had to test that it optimally binds to MS2 coat protein in vivo
star: false
creator: Graeme Glaister
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1791006_sequence/1

BBa_K1791007
type: ComponentDefinition
persistent identity: BBa K1791007
display id: BBa_K1791007
version: 1
was generated by: iGEM to SBOL conversion
description: BamHI cut site
created: 2015-09-22T11:00:00Z
modified: 2015-09-23T01:21:53Z
mutable provenance: https://www.neb.com/products/r0136-bamhi
owned by: james
owned by: myers
mutable description: This site will be utilized in the composite part BBa_K1791001 and BBa_K1791002 in order to allow for insertion of new RNA strands to be generated in these constructs.
discontinued: false
dominant: false
group u list: _2216_
m user id: 17031
owner id: 17031
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: no addtional design features were considered
star: false
creator: Graeme Glaister
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K1791007_sequence/1

BBa_K1791008
type: ComponentDefinition
persistent identity: BBa K1791008
display id: BBa_K1791008
version: 1
was generated by: iGEM to SBOL conversion
description: KpnI cut site
created: 2015-09-22T11:00:00Z
modified: 2015-09-23T01:29:59Z
mutable provenance: https://www.neb.com/products/r0142-kpni
owned by: james
owned by: myers
mutable description: This site will be utilized in the composite part BBa_K1791001 and BBa_K1791002 in order to allow for insertion of new RNA strands to be generated in these constructs.
discontinued: false
dominant: false
group u list: _2216_
m user id: 17031
owner id: 17031
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: no additional design considerations were neccessary
star: false
creator: Graeme Glaister
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K1791008_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1791001_sequence/1
type: Sequence
persistent identity: BBa K1791001 sequence
display id: BBa_K1791001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagaaagaggagaaatactagatggcaagcaacttcacgcagtttgtgctggtcgacaatggcggtaccggtgacgttacggttgccccaagcaacttcgccaacggtgtcgccgagtggatttccagcaactctcgctcccaggcatataaagtaacatgtagcgtgcgccagtcaagtgcacagaatcgcaagtacaccatcaaagtggaggtgccgaaagtcgcaacccagacggtgggcggtgttgaacttccggttgccgcgtggcgctcctatctgaatatggaactgaccatcccgatctttgctaccaattccgattgcgaattgattgtcaaagcgatgcaggggttactgaaggacggaaatccgattccaagtgcaattgctgctaacagcggaatctatcaccaccaccatcatcattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagggatcctactagagtaatacgactcactatagggagaaaacaaacaaagctgtcaccggatgtgctttccggtctgatgagtccgtgttgctgataccagcatcgtcttgatgcccttggcagcagtggacgaggacgaaacagcaaaaagaaaaataaaaaatactagagggtacctactagaggaaaagaaaacatgaggatcacccatgtcaaaagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I719005_sequence/1
type: Sequence
persistent identity: BBa I719005 sequence
display id: BBa_I719005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1791003_sequence/1
type: Sequence
persistent identity: BBa K1791003 sequence
display id: BBa_K1791003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcaagcaacttcacgcagtttgtgctggtcgacaatggcggtaccggtgacgttacggttgccccaagcaacttcgccaacggtgtcgccgagtggatttccagcaactctcgctcccaggcatataaagtaacatgtagcgtgcgccagtcaagtgcacagaatcgcaagtacaccatcaaagtggaggtgccgaaagtcgcaacccagacggtgggcggtgttgaacttccggttgccgcgtggcgctcctatctgaatatggaactgaccatcccgatctttgctaccaattccgattgcgaattgattgtcaaagcgatgcaggggttactgaaggacggaaatccgattccaagtgcaattgctgctaacagcggaatctatcaccaccaccatcatcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1791005_sequence/1
type: Sequence
persistent identity: BBa K1791005 sequence
display id: BBa_K1791005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagaaaacaaacaaagctgtcaccggatgtgctttccggtctgatgagtccgtgttgctgataccagcatcgtcttgatgcccttggcagcagtggacgaggacgaaacagcaaaaagaaaaataaaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1791006_sequence/1
type: Sequence
persistent identity: BBa K1791006 sequence
display id: BBa_K1791006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaaaagaaaacatgaggatcacccatgtcaaaagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1791007_sequence/1
type: Sequence
persistent identity: BBa K1791007 sequence
display id: BBa_K1791007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1791008_sequence/1
type: Sequence
persistent identity: BBa K1791008 sequence
display id: BBa_K1791008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z