LOCUS       BBa_K1792004             833 bp    DNA     linear       16-Jun-2024
DEFINITION  Nuclease - secreted with Cterm 6xHis tag
ACCESSION   BBa_K1792004
VERSION     BBa_K1792004.1  
FEATURES             Location/Qualifiers
     promoter        30..52
                     /label=T7 promoter
     misc_feature    55..75
                     /label=lac operator
     RBS             107..112
                     /label=RBS
     promoter        1..114
                     /label=BBa_K613010
     misc_feature    121..696
                     /label=BBa_K1792005
     stem_loop       716..759
                     /label=stem_loop
     misc_feature    705..784
                     /label=BBa_B0010
     terminator      705..784
                     /label=BBa_B0010
     stem_loop       800..819
                     /label=T7 TE
     misc_feature    826..826
                     /label=stop
     polyA_site      820..833
                     /label=polya
     misc_feature    793..833
                     /label=BBa_B0012
     terminator      793..833
                     /label=BBa_B0012
     terminator      705..833
                     /label=BBa_B0015
ORIGIN
        1 gaagcgagag gatcttaagg ctagagtacc aatacgactc actataggga gaggaattgt
       61 gagcggataa caattcccac tagaaataat tttgtttaac ttaagaagga ggaatactag
      121 atgaaaaaaa tttggttagc gctggcgggt ttggtcctgg ccttttctgc cagcgcgcag
      181 acggacaatg gagttaaccg tagtgggtca gaagatccga cagtgtattc agcgaccagc
      241 actaaaaaac ttcacaaaga accggcaacc ctgattaaag ccatcgacgg cgacacagtg
      301 aaattgatgt ataaaggtca gccgatgacc ttccgcctcc tgctggtgga taccccggaa
      361 accaaacatc cgaagaaggg tgtcgagaaa tacggtccgg aagccagcgc ctttacgaag
      421 aaaatggtgg aaaatgcgaa gaaaattgaa gtagaatttg ataaaggtca gcgcacggac
      481 aaatacgggc gtggtctggc ctatatttac gcggacggca aaatggttaa cgaagccttg
      541 gttcgtcagg gtctggcaaa agtggcatat gtttacaaac cgaataatac ccacgaacaa
      601 cacctgcgta aaagtgaagc gcaggcaaaa aaggaaaaat taaacatttg gagcgaagat
      661 aatgccgact ctggccaaca tcaccaccac caccattact agagccaggc atcaaataaa
      721 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
      781 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//